MRCK beta Antibody (D-7): sc-390076

MRCKβ Antibody (D-7) is a mouse monoclonal IgG3 antibody that detects MRCKβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MRCKβ, a member of the myotonic dystrophy kinase-related Cdc42-binding kinases family, plays a crucial role in regulating actin dynamics and cellular motility by phosphorylating nonmuscle myosin light chains, which in turn influences actin-myosin contractility. MRCKβ is primarily located in the cytoplasm, where MRCKβ interacts with various signaling pathways that are essential for processes such as cell migration and neurite outgrowth. The presence of a cysteine-rich motif and a pleckstrin homology domain in MRCKβ is significant as these structural features facilitate interaction with phospholipids and other proteins, thereby modulating activity in response to cellular signals. By understanding the function and localization of MRCKβ, researchers can gain insights into MRCKβ′s role in cellular processes and potential implications in diseases characterized by altered cell motility and cytoskeletal dynamics. Anti-MRCKβ antibody (D-7) is an invaluable tool for studying these mechanisms in various biological contexts.

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MRCK beta Antibody (D-7) References:

1. The actin-myosin regulatory MRCK kinases: regulation, biological functions and associations with human cancer.  |  Unbekandt, M. and Olson, MF. 2014. J Mol Med (Berl). 92: 217-25. PMID: 24553779
2. Cdc42 and k-Ras Control Endothelial Tubulogenesis through Apical Membrane and Cytoskeletal Polarization: Novel Stimulatory Roles for GTPase Effectors, the Small GTPases, Rac2 and Rap1b, and Inhibitory Influence of Arhgap31 and Rasa1.  |  Norden, PR., et al. 2016. PLoS One. 11: e0147758. PMID: 26812085
3. Molecular Signaling Pathways Controlling Vascular Tube Morphogenesis and Pericyte-Induced Tube Maturation in 3D Extracellular Matrices.  |  Bowers, SL., et al. 2016. Adv Pharmacol. 77: 241-80. PMID: 27451100
4. Regulation of Cdc42 and its effectors in epithelial morphogenesis.  |  Pichaud, F., et al. 2019. J Cell Sci. 132: PMID: 31113848
5. De novo heterozygous missense and loss-of-function variants in CDC42BPB are associated with a neurodevelopmental phenotype.  |  Chilton, I., et al. 2020. Am J Med Genet A. 182: 962-973. PMID: 32031333
6. Spatiotemporal control of actomyosin contractility by MRCKβ signaling drives phagocytosis.  |  Zihni, C., et al. 2022. J Cell Biol. 221: PMID: 36121394
7. Opportunities and Challenges for the Development of MRCK Kinases Inhibitors as Potential Cancer Chemotherapeutics.  |  Ruscetta, VM., et al. 2023. Cells. 12: PMID: 36831201
8. MRCKα/β positively regulates Gli protein activity.  |  Baran, B., et al. 2023. Cell Signal. 107: 110666. PMID: 37019250
9. Zhuidu Formula suppresses the migratory and invasive properties of triple-negative breast cancer cells via dual signaling pathways of RhoA/ROCK and CDC42/MRCK.  |  Wu, Q., et al. 2023. J Ethnopharmacol. 315: 116644. PMID: 37196814
10. MRCK ensures cortex-chromatin 'social distancing' to enable egg spindle rotation.  |  Totsuka, T., et al. 2023. J Cell Biol. 222: PMID: 37843812

The preferred MRCKβ antibody is MRCKβ Antibody (C-12): sc-374597.