IL-10 Antibody (JES5-2A5): sc-52561
- IL-10 Antibody (JES5-2A5) is a rat monoclonal IgG1, cited in 9 publications, provided at 100 µg/ml
- raised against recombinant mouse IL-10
- recommended for detection of IL-10 of mouse origin by WB, IF and FCM
- See IL-10 (A-2): sc-365858 for IL-10 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-10 Antibody (JES5-2A5). This work is in progress.
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IL-10 Antibody (JES5-2A5) is a rat monoclonal IgG1 antibody that detects interleukin-10 (IL-10) in mouse samples through applications such as western blotting (WB), immunofluorescence (IF), and flow cytometry (FCM). IL-10 is a crucial cytokine primarily secreted by TH2 clones, playing a significant role in immune regulation by suppressing the production of pro-inflammatory cytokines from TH1 clones in response to antigens presented by monocyte and macrophage antigen presenting cells. This suppression is vital for maintaining immune homeostasis and preventing excessive inflammatory responses that can lead to tissue damage. Additionally, IL-10 inhibits the activation of cytokine-targeted cells, potentially through the downregulation of CD25, the IL-2 receptor, on macrophages and B lymphocytes, further contributing to its immunosuppressive effects. Anti-IL-10 antibody (JES5-2A5) recognizes both human and murine IL-10, which share a high degree of sequence identity, and is particularly useful in studies investigating the role of IL-10 in various diseases, including its elevated expression in HIV-1 infected individuals, where IL-10 has been implicated in disease progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IL-10 Antibody (JES5-2A5) References:
- IL-10 reduces Th2 cytokine production and eosinophilia but augments airway reactivity in allergic mice. | van Scott, MR., et al. 2000. Am J Physiol Lung Cell Mol Physiol. 278: L667-74. PMID: 10749743
- Structure of IL-10 and its role in autoimmune exocrinopathy. | Saito, I. 2000. Crit Rev Immunol. 20: 153-65. PMID: 10872895
- IL-10 secretion and sensitivity in normal human intestine and inflammatory bowel disease. | Gasche, C., et al. 2000. J Clin Immunol. 20: 362-70. PMID: 11051278
- Expression patterns of IL-10 ligand and receptor gene families provide leads for biological characterization. | Nagalakshmi, ML., et al. 2004. Int Immunopharmacol. 4: 577-92. PMID: 15120644
- Pivotal role of PGE2 and IL-10 in the cross-regulation of dendritic cell-derived inflammatory mediators. | Harizi, H. and Gualde, N. 2006. Cell Mol Immunol. 3: 271-7. PMID: 16978535
- IL-10 deficiency unleashes an influenza-specific Th17 response and enhances survival against high-dose challenge. | McKinstry, KK., et al. 2009. J Immunol. 182: 7353-63. PMID: 19494257
- Epstein-Barr virus IL-10 engages IL-10R1 by a two-step mechanism leading to altered signaling properties. | Yoon, SI., et al. 2012. J Biol Chem. 287: 26586-95. PMID: 22692218
- The association between interleukin-10 (IL-10) -592C/A, -819T/C, -1082G/A promoter polymorphisms and endometriosis. | Malutan, AM., et al. 2017. Arch Gynecol Obstet. 295: 503-510. PMID: 28004192
- AAV6-Mediated IL-10 Expression in the Lung Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice. | Kurosaki, F., et al. 2018. Hum Gene Ther. 29: 1242-1251. PMID: 29598007
- The role of interleukin-10 family members in cardiovascular diseases. | Xu, S., et al. 2021. Int Immunopharmacol. 94: 107475. PMID: 33662690
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-10 Antibody (JES5-2A5) | sc-52561 | 100 µg/ml | $322.00 |