IL-10 Antibody (JES5-16E3): sc-52560
- IL-10 Antibody (JES5-16E3) is a rat monoclonal IgG2b, cited in 2 publications, provided at 100 µg/ml
- raised against recombinant full length IL-10 of mouse origin
- recommended for detection of IL-10 of mouse origin by WB, IP and ELISA
- See IL-10 (A-2): sc-365858 for IL-10 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for IL-10 Antibody (JES5-16E3). This work is in progress.
QUICK LINKS
SEE ALSO...
IL-10 Antibody (JES5-16E3) is a rat monoclonal IgG2b antibody that detects interleukin-10 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-IL-10 antibody (JES5-16E3) recognizes interleukin-10, a crucial 178 amino acid protein primarily secreted by TH2 clones, which plays a vital role in immune regulation. This cytokine suppresses the production of pro-inflammatory cytokines by TH1 clones in response to antigens presented by monocyte and macrophage antigen presenting cells, thereby maintaining immune homeostasis. Additionally, interleukin-10 inhibits the response of cytokine-targeted cells, potentially through the downregulation of CD25, the IL-2 receptor, on macrophages and B lymphocytes. Interleukin-10 exists as a non-covalently linked homodimer in solution, which is essential for biological activity. The interaction of interleukin-10 with IL-10 receptor (IL-10R), a glycoprotein that can be induced in cultured macrophages and fibroblasts by lipopolysaccharide stimulation, further underscores its importance in modulating immune responses. Notably, elevated levels of interleukin-10 have been observed in individuals infected with HIV-1, suggesting involvement in disease progression, making this protein critical for understanding various immune-related conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IL-10 Antibody (JES5-16E3) References:
- Lessons from genetically engineered animal models. XII. IL-10-deficient (IL-10(-/-) mice and intestinal inflammation. | Rennick, DM. and Fort, MM. 2000. Am J Physiol Gastrointest Liver Physiol. 278: G829-33. PMID: 10859210
- IL-10, a key effector regulatory cytokine in experimental autoimmune encephalomyelitis. | Bettelli, E., et al. 2003. J Autoimmun. 20: 265-7. PMID: 12791309
- mda-7/IL-24: a unique member of the IL-10 gene family promoting cancer-targeted toxicity. | Dash, R., et al. 2010. Cytokine Growth Factor Rev. 21: 381-91. PMID: 20926331
- Inhalative IL-10 treatment after bilateral femoral fractures affect pulmonary inflammation in mice. | Lichte, P., et al. 2015. Ann Anat. 200: 73-8. PMID: 25801583
- Insufficient IL-10 Production as a Mechanism Underlying the Pathogenesis of Systemic Juvenile Idiopathic Arthritis. | Imbrechts, M., et al. 2018. J Immunol. 201: 2654-2663. PMID: 30266771
- Interleukin-10 Family Cytokines Immunobiology and Structure. | Wei, H., et al. 2019. Adv Exp Med Biol. 1172: 79-96. PMID: 31628652
- Upregulation of IL-10 expression inhibits the proliferation of human periodontal ligament stem cells. | Liu, Y., et al. 2020. Braz Oral Res. 34: e030. PMID: 32236319
- Anti-inflammatory IL-10 administration rescues depression-associated learning and memory deficits in mice. | Worthen, RJ., et al. 2020. J Neuroinflammation. 17: 246. PMID: 32828124
- Mesenchymal stem cells overexpressing interleukin-10 prevent allergic airway inflammation. | Kuang, PP., et al. 2023. Stem Cell Res Ther. 14: 369. PMID: 38093354
- Regulation of IL-10 expression and function by JAK-STAT in CD8+ T cells. | Pei, Y., et al. 2024. Int Immunopharmacol. 128: 111563. PMID: 38246002
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-10 Antibody (JES5-16E3) | sc-52560 | 100 µg/ml | $322.00 |