Herc1 Antibody (E-12): sc-393950
- Herc1 Antibody (E-12) is a mouse monoclonal IgG1 κ Herc1 antibody provided at 200 µg/ml
- raised against amino acids 591-890 mapping near the N-terminus of Herc1 of human origin
- Herc1 Antibody (E-12) is recommended for detection of Herc1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
- Anti-Herc1 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Herc1 Antibody (E-12) for WB applications. These reagents are now offered in bundles with Herc1 Antibody (E-12) (see ordering information below).
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Herc1 Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Herc1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Herc1 antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Herc1, also known as p532 or p619, is one of the largest human proteins, comprising 4,861 amino acids and featuring a diverse array of functional domains such as WD repeats, RCC1 repeats, beta-repeat domains, and a HECT domain. Herc1 is crucial for cellular processes, as Herc1 is involved in membrane trafficking by acting as a guanine nucleotide exchange factor for ARF1 and Rab proteins, which are essential for the regulation of vesicle transport within cells. Herc1 localization to the cytosol and Golgi apparatus underscores Herc1′s role in facilitating the movement of proteins and lipids, thereby influencing cellular communication and metabolism. Additionally, Herc1 is implicated in cell proliferation and growth through interactions with proteins like clathrin, M2-pyruvate kinase, and TSC2. Notably, mutations in TSC2 can lead to TSC2 binding with Herc1 in the presence of TSC1, resulting in TSC2 destabilization and contributing to the development of tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by the formation of benign tumors in various organs.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Herc1 Antibody (E-12) References:
- Assignment of the human P532 gene (HERC1) to chromosome 15q22 by fluorescence in situ hybridization. | Cruz, C., et al. 1999. Cytogenet Cell Genet. 86: 68-9. PMID: 10516438
- Interaction between HERC1 and M2-type pyruvate kinase. | Garcia-Gonzalo, FR., et al. 2003. FEBS Lett. 539: 78-84. PMID: 12650930
- The giant protein HERC1 is recruited to aluminum fluoride-induced actin-rich surface protrusions in HeLa cells. | Garcia-Gonzalo, FR., et al. 2004. FEBS Lett. 559: 77-83. PMID: 14960311
- Requirement of phosphatidylinositol-4,5-bisphosphate for HERC1-mediated guanine nucleotide release from ARF proteins. | Garcia-Gonzalo, FR., et al. 2005. FEBS Lett. 579: 343-8. PMID: 15642342
- TSC1 stabilizes TSC2 by inhibiting the interaction between TSC2 and the HERC1 ubiquitin ligase. | Chong-Kopera, H., et al. 2006. J Biol Chem. 281: 8313-6. PMID: 16464865
- Progressive Purkinje cell degeneration in tambaleante mutant mice is a consequence of a missense mutation in HERC1 E3 ubiquitin ligase. | Mashimo, T., et al. 2009. PLoS Genet. 5: e1000784. PMID: 20041218
- p619, a giant protein related to the chromosome condensation regulator RCC1, stimulates guanine nucleotide exchange on ARF1 and Rab proteins. | Rosa, JL., et al. 1996. EMBO J. 15: 4262-73. PMID: 8861955
- A giant protein that stimulates guanine nucleotide exchange on ARF1 and Rab proteins forms a cytosolic ternary complex with clathrin and Hsp70. | Rosa, JL. and Barbacid, M. 1997. Oncogene. 15: 1-6. PMID: 9233772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Herc1 Antibody (E-12) | sc-393950 | 200 µg/ml | $322.00 | |||
Herc1 Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-527723 | 200 µg Ab; 10 µg BP | $361.00 | |||
Herc1 Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-533096 | 200 µg Ab; 20 µg BP | $361.00 | |||
Herc1 Antibody (E-12) AC | sc-393950 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Herc1 Antibody (E-12) HRP | sc-393950 HRP | 200 µg/ml | $322.00 | |||
Herc1 Antibody (E-12) FITC | sc-393950 FITC | 200 µg/ml | $336.00 | |||
Herc1 Antibody (E-12) PE | sc-393950 PE | 200 µg/ml | $349.00 | |||
Herc1 Antibody (E-12) Alexa Fluor® 488 | sc-393950 AF488 | 200 µg/ml | $364.00 | |||
Herc1 Antibody (E-12) Alexa Fluor® 546 | sc-393950 AF546 | 200 µg/ml | $364.00 | |||
Herc1 Antibody (E-12) Alexa Fluor® 594 | sc-393950 AF594 | 200 µg/ml | $364.00 | |||
Herc1 Antibody (E-12) Alexa Fluor® 647 | sc-393950 AF647 | 200 µg/ml | $364.00 | |||
Herc1 Antibody (E-12) Alexa Fluor® 680 | sc-393950 AF680 | 200 µg/ml | $364.00 | |||
Herc1 Antibody (E-12) Alexa Fluor® 790 | sc-393950 AF790 | 200 µg/ml | $364.00 |