EEA1 Antibody (281.7): sc-53939

EEA1 Antibody (281.7) is a mouse monoclonal IgG1 antibody that detects EEA1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. EEA1, or early endosome antigen 1, plays a crucial role in the early stages of endocytosis by facilitating the fusion of early endosomes and is essential for the sorting and transport of internalized proteins. EEA1 is primarily located in the cytoplasm, where EEA1 associates with early endosomes, and proper localization is vital for maintaining cellular homeostasis and regulating membrane trafficking pathways. EEA1 contains specific structural motifs, including a calmodulin-binding IQ motif and a cysteine-rich finger motif, which are critical for interaction with other proteins and localization to early endosomes. Additionally, EEA1 has sequence homology with several yeast proteins involved in membrane trafficking, such as Vps27, Fab1, and Vac1, highlighting evolutionary conservation and importance in cellular processes. EEA1′s ability to mediate the regulatory effects of 3′-phosphoinositides on membrane trafficking further underscores significance in cellular dynamics, making anti-EEA1 antibody (281.7) an invaluable tool for researchers studying endosomal pathways and their implications in various biological contexts.

The preferred EEA1 antibody is EEA1 Antibody (E-8): sc-365652.