EDAR Antibody (G-1): sc-271627

EDAR Antibody (G-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects EDAR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EDAR antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ectodermal dysplasia receptor (EDAR) plays a crucial role in ectodermal differentiation and is a member of the tumor necrosis factor receptor (TNFR) superfamily, which consists of type I transmembrane glycoproteins involved in essential cellular functions such as proliferation, differentiation, and programmed cell death. EDAR is encoded by the downless gene and is known to be mutated in ectodermal dysplasia syndromes, which lead to developmental issues in hair, teeth, and sweat glands. EDAR′s structure contains cysteine-rich repeats in its extracellular ligand-binding domain and an intracellular death domain, allowing interactions with various signaling pathways. These interactions can activate either pro-apoptotic pathways, such as caspase and Jun kinase, or promote cell survival through the NFkB pathway. EDAR interacts with ectodysplasin A (EDA), a type II membrane protein encoded by the Tabby gene, which serves as its ligand. The longest splice variant of EDA, EDA-A1, binds to EDAR, while EDA-A2, which has a two-amino-acid deletion, specifically interacts with the X-linked ectodysplasin-A2 receptor (XEDAR). Both EDAR and XEDAR share homology with TROY, further emphasizing the complexity and significance of these protein interactions in cellular signaling and development.

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EDAR Antibody (G-1) References:

1. TROY, a newly identified member of the tumor necrosis factor receptor superfamily, exhibits a homology with Edar and is expressed in embryonic skin and hair follicles.  |  Kojima, T., et al. 2000. J Biol Chem. 275: 20742-7. PMID: 10764796
2. Edar/Eda interactions regulate enamel knot formation in tooth morphogenesis.  |  Tucker, AS., et al. 2000. Development. 127: 4691-700. PMID: 11023871
3. The ectodermal dysplasia receptor activates the nuclear factor-kappaB, JNK, and cell death pathways and binds to ectodysplasin A.  |  Kumar, A., et al. 2001. J Biol Chem. 276: 2668-77. PMID: 11035039
4. Two-amino acid molecular switch in an epithelial morphogen that regulates binding to two distinct receptors.  |  Yan, M., et al. 2000. Science. 290: 523-7. PMID: 11039935
5. Ectodysplasin is released by proteolytic shedding and binds to the EDAR protein.  |  Elomaa, O., et al. 2001. Hum Mol Genet. 10: 953-62. PMID: 11309369
6. Edar signaling in the control of hair follicle development.  |  Botchkarev, VA. and Fessing, MY. 2005. J Investig Dermatol Symp Proc. 10: 247-51. PMID: 16382675
7. Signaling involved in hair follicle morphogenesis and development.  |  Rishikaysh, P., et al. 2014. Int J Mol Sci. 15: 1647-70. PMID: 24451143
8. Edar is a downstream target of beta-catenin and drives collagen accumulation in the mouse prostate.  |  Wegner, KA., et al. 2019. Biol Open. 8: PMID: 30745437
9. The EDA/EDAR/NF-κB pathway in non-syndromic tooth agenesis: A genetic perspective.  |  Gao, Y., et al. 2023. Front Genet. 14: 1168538. PMID: 37077539
10. Structural and biological features of the TNF receptor and TNF ligand superfamilies: interactive signals in the pathobiology of Hodgkin's disease.  |  Gruss, HJ., et al. 1996. Ann Oncol. 7 Suppl 4: 19-26. PMID: 8836404
11. Molecular, structural, and biological characteristics of the tumor necrosis factor ligand superfamily.  |  Gruss, HJ. 1996. Int J Clin Lab Res. 26: 143-59. PMID: 8905447
12. Modulation of life and death by the TNF receptor superfamily.  |  Baker, SJ. and Reddy, EP. 1998. Oncogene. 17: 3261-70. PMID: 9916988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EDAR Antibody (G-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-EDAR Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EDAR Antibody (G-1) Specifications

Alternate Product Name: EDAR

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 415-448

Epitope Target: EDAR

Epitope Species: human

Product Reactivity/Detection: EDAR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for EDAR Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin