DNA pol epsilon B Antibody (B-3): sc-376703

DNA pol ε B Antibody (B-3) is a mouse monoclonal IgG3 antibody that detects DNA polymerase epsilon (DNA pol ε) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA pol ε plays a crucial role in DNA replication, particularly in the synthesis of the leading strand, ensuring accurate and efficient duplication of the genome. Anti-DNA pol ε antibody (B-3) recognizes a multi-subunit complex, with its catalytic subunit encoded by the POL2 gene, which is essential for maintaining genomic stability. Proper functioning of DNA pol ε is vital, as errors in DNA replication can lead to mutations and genomic instability, which are hallmarks of various diseases, including cancer. DNA pol ε is subject to regulation by cell cycle proteins, which modulate activity in response to cellular needs, highlighting importance in coordinating DNA synthesis with the cell cycle. DNA pol ε B (B-3) antibody serves as an invaluable tool for researchers studying mechanisms of DNA replication and repair, as well as implications of these processes in health and disease.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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DNA pol epsilon B Antibody (B-3) References:

1. Eukaryotic DNA polymerases.  |  Hubscher, U., et al. 2002. Annu Rev Biochem. 71: 133-63. PMID: 12045093
2. DNA replicases from a bacterial perspective.  |  McHenry, CS. 2011. Annu Rev Biochem. 80: 403-36. PMID: 21675919
3. DNA polymerase ε.  |  Hogg, M. and Johansson, E. 2012. Subcell Biochem. 62: 237-57. PMID: 22918589
4. Reconstitution of a eukaryotic replisome reveals the mechanism of asymmetric distribution of DNA polymerases.  |  Yurieva, O. and O'Donnell, M. 2016. Nucleus. 7: 360-8. PMID: 27416113
5. DNA Polymerase Epsilon Deficiency Causes IMAGe Syndrome with Variable Immunodeficiency.  |  Logan, CV., et al. 2018. Am J Hum Genet. 103: 1038-1044. PMID: 30503519
6. Recombination and Pol ζ Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ε Interaction.  |  Denkiewicz-Kruk, M., et al. 2020. Int J Mol Sci. 21: PMID: 33322195
7. Yeast 9-1-1 complex acts as a sliding clamp for DNA synthesis by DNA polymerase ε.  |  Acharya, N., et al. 2023. J Biol Chem. 299: 102727. PMID: 36410434
8. CAF-1 deposits newly synthesized histones during DNA replication using distinct mechanisms on the leading and lagging strands.  |  Rouillon, C., et al. 2023. Nucleic Acids Res. 51: 3770-3792. PMID: 36942484
9. Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand.  |  Dmowski, M., et al. 2023. DNA Repair (Amst). 129: 103541. PMID: 37481989
10. RNA binding protein TIAR modulates HBV replication by tipping the balance of pgRNA translation.  |  Zhang, T., et al. 2023. Signal Transduct Target Ther. 8: 346. PMID: 37699883

The preferred DNA pol ε B antibody is DNA pol ε B Antibody (C-9): sc-398582.