CCNB1/cyclin B1 Antibody (V152): sc-53236
- CCNB1/cyclin B1 Antibody (V152) is a mouse monoclonal IgG1 κ, cited in 11 publications, provided at 200 µg/ml
- raised against His-tagged recombinant cyclin B1 of hamster origin
- recommended for detection of cyclin B1 of mouse, rat, human and hamster origin by WB, IP, IF, IHC(P) and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See CCNB1/cyclin B1 (GNS1): sc-245 for CCNB1/cyclin B1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CCNB1/cyclin B1 Antibody (V152) for WB and IHC(P) applications. These reagents are now offered in bundles with CCNB1/cyclin B1 Antibody (V152) (see ordering information below).
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cyclin B1 Antibody (V152) is a mouse monoclonal IgG1 kappa light chain antibody raised against His-tagged recombinant cyclin B1 of hamster origin, reacting with mouse, rat, hamster, and human species. Cyclin B1, also known as G2/mitotic-specific cyclin-B1 or CCNB1, is a crucial regulatory protein that controls the transition from G2 phase to mitosis in the cell cycle by forming a complex with cyclin-dependent kinase 1 (CDK1). This complex, known as the maturation-promoting factor (MPF), initiates mitosis by phosphorylating target substrates essential for cell division. Cyclin B1 undergoes significant post-translational modifications, including phosphorylation and ubiquitination, which regulate activity, localization, and degradation. Phosphorylation affects interaction with CDK1 and nuclear translocation, while ubiquitination targets for proteasomal degradation, ensuring proper cell cycle progression. The precise regulation of these modifications is vital for maintaining normal cell proliferation, and dysregulation can lead to uncontrolled growth and cancer. Anti-cyclin B1 antibody (V152) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). By targeting cyclin B1, researchers can study expression, post-translational modifications, and role in cell cycle regulation, contributing to a better understanding of cellular proliferation and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CCNB1/cyclin B1 Antibody (V152) References:
- Cyclin B1 and other cyclins as tumor antigens in immunosurveillance and immunotherapy of cancer. | Egloff, AM., et al. 2006. Cancer Res. 66: 6-9. PMID: 16397206
- Cyclin A2 regulates nuclear-envelope breakdown and the nuclear accumulation of cyclin B1. | Gong, D., et al. 2007. Curr Biol. 17: 85-91. PMID: 17208191
- Cyclin B1-Cdk1 activation continues after centrosome separation to control mitotic progression. | Lindqvist, A., et al. 2007. PLoS Biol. 5: e123. PMID: 17472438
- Cyclin B1 expression regulated by cytoplasmic polyadenylation element binding protein in astrocytes. | Kim, KC., et al. 2011. J Neurosci. 31: 12118-28. PMID: 21865454
- The prognostic value of Cyclin B1 in pancreatic cancer. | Zhou, L., et al. 2014. Med Oncol. 31: 107. PMID: 25106528
- MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. | Voets, E. and Wolthuis, R. 2015. Biol Open. 4: 484-95. PMID: 25750436
- CYCLIN-B1/2 and -D1 act in opposition to coordinate cortical progenitor self-renewal and lineage commitment. | Hagey, DW., et al. 2020. Nat Commun. 11: 2898. PMID: 32518258
- Oxidative Stress-Induced Unscheduled CDK1-Cyclin B1 Activity Impairs ER-Mitochondria-Mediated Bioenergetic Metabolism. | Chang, JG., et al. 2021. Cells. 10: PMID: 34064109
- Macrophages maintain mammary stem cell activity and mammary homeostasis via TNF-α-PI3K-Cdk1/Cyclin B1 axis. | Zhou, Y., et al. 2023. NPJ Regen Med. 8: 23. PMID: 37130846
- A pancancer analysis of the oncogenic role of cyclin B1 (CCNB1) in human tumors. | Dai, P., et al. 2023. Sci Rep. 13: 16226. PMID: 37758792
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCNB1/cyclin B1 Antibody (V152) | sc-53236 | 200 µg/ml | $322.00 | |||
CCNB1/cyclin B1 Antibody (V152): m-IgG Fc BP-HRP Bundle | sc-536913 | 200 µg Ab; 10 µg BP | $361.00 | |||
CCNB1/cyclin B1 Antibody (V152): m-IgGκ BP-HRP Bundle | sc-534086 | 200 µg Ab; 40 µg BP | $361.00 | |||
CCNB1/cyclin B1 Antibody (V152): m-IgG1 BP-HRP Bundle | sc-544894 | 200 µg Ab; 20 µg BP | $361.00 | |||
CCNB1/cyclin B1 Antibody (V152) FITC | sc-53236 FITC | 200 µg/ml | $336.00 | |||
CCNB1/cyclin B1 Antibody (V152) PE | sc-53236 PE | 200 µg/ml | $349.00 |