CD47 Antibody (6A296): sc-70716

CD47 Antibody (6A296) is a mouse monoclonal IgG1 antibody that detects CD47 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). CD47 is an integral membrane protein that plays a crucial role in regulating cation fluxes across cell membranes, particularly influencing intracellular calcium concentrations during cell adhesion to the extracellular matrix. This function is vital as it affects various cellular processes, including migration, proliferation, and survival. Additionally, CD47 serves as a receptor for the C-terminal cell binding domain of thrombospondin, which is important for mediating cell-cell interactions and signaling pathways. Notably, CD47 is absent from Rh-null erythrocytes, highlighting its significance in cell adhesion mechanisms in non-erythroid cells and its potential role in preventing the premature elimination of erythrocytes. Furthermore, CD47 is expressed on a variety of cell types, including hemopoietic cells, epithelial cells, endothelial cells, and fibroblasts, with particularly strong expression in brain and mesenchymal cells, underscoring its importance in both normal physiology and disease states. Anti-CD47 antibody (6A296) is an essential tool for researchers investigating the multifaceted roles of CD47 in cellular processes and its implications in various pathologies.

The preferred CD47 antibody is CD47 Antibody (B6H12): sc-12730.