CD3-epsilon Antibody (145-2C11): sc-18871

CD3-ε Antibody (145-2C11) is a hamster monoclonal IgG antibody that detects CD3-ε of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD3-ε (145-2C11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD3-ε plays a crucial role in the immune response as a component of the T cell receptor (TCR) complex, which is essential for T cell activation and signaling. CD3-ε is located on the surface of T cells, where CD3-ε forms part of a multisubunit complex that transmits signals upon recognition of foreign antigens. CD3-ε initiates a cascade of intracellular signaling events that lead to T cell activation, proliferation, and differentiation, which are vital for an effective immune response. The CD3 complex, which includes CD3-ε, consists of five invariant polypeptide chains that form three distinct dimers, facilitating the transmission of signals that ultimately determine the fate of T cells in immune responses. Understanding the function and signaling mechanisms of CD3-ε is critical for developing therapeutic strategies targeting T cell-mediated diseases and enhancing immune responses in various clinical settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD3-epsilon Antibody (145-2C11) References:

1. Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3.  |  Kjer-Nielsen, L., et al. 2004. Proc Natl Acad Sci U S A. 101: 7675-80. PMID: 15136729
2. The membrane-proximal portion of CD3 epsilon associates with the serine/threonine kinase GRK2.  |  DeFord-Watts, LM., et al. 2007. J Biol Chem. 282: 16126-34. PMID: 17420248
3. Loss of N-terminal charged residues of mouse CD3 epsilon chains generates isoforms modulating antigen T cell receptor-mediated signals and T cell receptor-CD3 interactions.  |  Bello, R., et al. 2007. J Biol Chem. 282: 22324-34. PMID: 17561508
4. Structure of the T-cell antigen receptor: evidence for two CD3 epsilon subunits in the T-cell receptor-CD3 complex.  |  Blumberg, RS., et al. 1990. Proc Natl Acad Sci U S A. 87: 7220-4. PMID: 2144901
5. An enhancer located in a CpG-island 3' to the TCR/CD3-epsilon gene confers T lymphocyte-specificity to its promoter.  |  Clevers, H., et al. 1989. EMBO J. 8: 2527-35. PMID: 2583122
6. Interplay between the human TCR/CD3 epsilon and the B-cell antigen receptor associated Ig-beta (B29).  |  Müller, B., et al. 1995. Immunol Lett. 44: 97-103. PMID: 7541024
7. Altered T cell development in mice with a targeted mutation of the CD3-epsilon gene.  |  Malissen, M., et al. 1995. EMBO J. 14: 4641-53. PMID: 7588594
8. Compared TCR and CD3 epsilon expression on alpha beta and gamma delta T cells. Evidence for the association of two TCR heterodimers with three CD3 epsilon chains in the TCR/CD3 complex.  |  Thibault, G. and Bardos, P. 1995. J Immunol. 154: 3814-20. PMID: 7706721
9. CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression.  |  Shinkai, Y. and Alt, FW. 1994. Int Immunol. 6: 995-1001. PMID: 7947468
10. T lymphocyte development in the absence of CD3 epsilon or CD3 gamma delta epsilon zeta.  |  Wang, B., et al. 1999. J Immunol. 162: 88-94. PMID: 9886373

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3-ε Antibody (145-2C11) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3-ε Antibody (145-2C11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3-ε Antibody (145-2C11) Specifications

Alternate Product Name: CD3 epsilon

Product Clone Name: 145-2C11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: CD3-ε

Epitope Species: mouse

Product Reactivity/Detection: CD3-ε

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CD3-ε Antibody (145-2C11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin

The preferred CD3-ε antibody is CD3-ε Antibody (UCH-T1): sc-1179.