Cbl Antibody (G-12): sc-398282

Cbl Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the Cbl protein, also known as c-Cbl, CBL proto-oncogene, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, and RING finger protein 55. Cbl monoclonal antibody (G-12) targets an epitope encompassing amino acids 607-906 at the C-terminus of the human Cbl protein, ensuring precise and reliable binding. The Cbl protein functions as an E3 ubiquitin ligase, playing a critical role in regulating various signaling pathways by ubiquitinating target proteins such as epidermal growth factor receptor (EGFR), Src family kinases, and the insulin receptor. These interactions are essential for controlling processes like cell proliferation, differentiation, and apoptosis, thereby maintaining cellular homeostasis and preventing oncogenic transformations. Anti-Cbl antibody (G-12) exhibits robust reactivity with Cbl proteins from mouse, rat, and human species, making Cbl monoclonal antibody (G-12) suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Cbl antibody (G-12) is available in both non-conjugated form and various conjugated forms, such as horseradish peroxidase (HRP) and Alexa Fluor® variants, providing flexibility for diverse experimental requirements. Cbl (G-12) antibody′s high affinity and specificity make this reagent an invaluable tool for researchers studying the intricate mechanisms of ubiquitination and signal transduction, as well as investigating the molecular underpinnings of diseases like cancer and autoimmune disorders. By enabling the precise detection of Cbl interactions with key signaling proteins, anti-Cbl antibody (G-12) supports advancements in understanding and targeting critical pathways involved in cellular regulation and disease progression.

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Cbl Antibody (G-12) References:

1. The Cbl interactome and its functions.  |  Schmidt, MHH. and Dikic, I. 2005. Nat Rev Mol Cell Biol. 6: 907-18. PMID: 16227975
2. Germline CBL mutations cause developmental abnormalities and predispose to juvenile myelomonocytic leukemia.  |  Niemeyer, CM., et al. 2010. Nat Genet. 42: 794-800. PMID: 20694012
3. Cbl proteins in platelet activation.  |  Buitrago, L., et al. 2013. Platelets. 24: 419-27. PMID: 22931288
4. Cbl-family proteins as regulators of cytoskeleton-dependent phenomena.  |  Lee, H. and Tsygankov, AY. 2013. J Cell Physiol. 228: 2285-93. PMID: 23744514
5. Molecular pathways: cbl proteins in tumorigenesis and antitumor immunity-opportunities for cancer treatment.  |  Liyasova, MS., et al. 2015. Clin Cancer Res. 21: 1789-94. PMID: 25477533
6. CBL-CIPK module-mediated phosphoregulation: facts and hypothesis.  |  Sanyal, SK., et al. 2020. Biochem J. 477: 853-871. PMID: 32129820
7. The CBL-CIPK Pathway in Plant Response to Stress Signals.  |  Ma, X., et al. 2020. Int J Mol Sci. 21: PMID: 32784662
8. CBL mutations drive PI3K/AKT signaling via increased interaction with LYN and PIK3R1.  |  Belizaire, R., et al. 2021. Blood. 137: 2209-2220. PMID: 33512474
9. Role of CBL Mutations in Cancer and Non-Malignant Phenotype.  |  Leardini, D., et al. 2022. Cancers (Basel). 14: PMID: 35159106
10. Cbl and Cbl-b independently regulate EGFR through distinct receptor interaction modes.  |  Pinilla-Macua, I. and Sorkin, A. 2023. Mol Biol Cell. 34: ar134. PMID: 37903221

The preferred Cbl antibody is Cbl Antibody (A-9): sc-1651.