caspase-9 p10 Antibody (F-7): sc-17784

caspase-9 p10 Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects the precursor and p10 subunit of caspase-9 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-caspase-9 p10 antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-9 is a crucial component of the apoptotic pathway, functioning as an initiator caspase that activates downstream effector caspases, such as caspase-3 and caspase-7, in response to pro-apoptotic signals. This activation is vital for the execution of programmed cell death, which is essential for maintaining cellular homeostasis and eliminating damaged or unwanted cells. Proper regulation of caspase-9 activity is critical, as dysregulation can lead to various diseases, including cancer, where evasion of apoptosis allows for uncontrolled cell proliferation. Caspase-9 interacts with several proteins, including Apaf-1, which forms the apoptosome complex that facilitates caspase-9 activation in response to cytochrome c release from mitochondria. Understanding these interactions and regulatory mechanisms involving caspase-9 is important for developing therapeutic strategies aimed at modulating apoptosis in disease contexts.

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caspase-9 p10 Antibody (F-7) References:

1. Regulation of the Apaf-1/caspase-9 apoptosome by caspase-3 and XIAP.  |  Zou, H., et al. 2003. J Biol Chem. 278: 8091-8. PMID: 12506111
2. Caspase-9: structure, mechanisms and clinical application.  |  Li, P., et al. 2017. Oncotarget. 8: 23996-24008. PMID: 28177918
3. Caspase-9: A Multimodal Therapeutic Target With Diverse Cellular Expression in Human Disease.  |  Avrutsky, MI. and Troy, CM. 2021. Front Pharmacol. 12: 701301. PMID: 34305609
4. Caspase-9 inhibition triggers Hsp90-based chemotherapy-mediated tumor intrinsic innate sensing and enhances antitumor immunity.  |  Li, J., et al. 2023. J Immunother Cancer. 11: PMID: 38056894
5. Post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks.  |  Lindahl, T., et al. 1995. Trends Biochem Sci. 20: 405-11. PMID: 8533153
6. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
7. Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death.  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
8. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
9. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
10. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
11. The expression of poly(ADP-ribose) polymerase during differentiation-linked DNA replication reveals that it is a component of the multiprotein DNA replication complex.  |  Simbulan-Rosenthal, CM., et al. 1996. Biochemistry. 35: 11622-33. PMID: 8794742
12. Caspases: the executioners of apoptosis.  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-9 p10 Antibody (F-7) has 104 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-9 p10 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-9 p10 Antibody (F-7) Specifications

Alternate Product Name: caspase-9 p10

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 315-397

Epitope Target: caspase-9 p10

Epitope Species: human

Product Reactivity/Detection: precursor and p10 subunit of caspase-9

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 104 citations in various scientific publications

Available Conjugates/Forms for caspase-9 p10 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred caspase-9 antibody is caspase-9 Antibody (96.1.23): sc-56076.