BRD4 Antibody (C-2): sc-518158
- BRD4 Antibody (C-2) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 662-683 of BRD4 of human origin
- recommended for detection of BRD4 of human origin by WB, IP, IF and ELISA
- Anti-BRD4 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for BRD4 Antibody (C-2) for WB applications. This reagent is now offered in a bundle with BRD4 Antibody (C-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
BRD4 Antibody (C-2) is a mouse monoclonal IgM kappa antibody that detects BRD4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BRD4 Antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BRD4, a member of the bromodomain and extraterminal (BET) family, plays a crucial role in regulating gene expression and is primarily located in the nucleus, where BRD4 interacts with acetylated lysines on histones and non-histone proteins. This nuclear localization is vital for BRD4′s function in transcriptional regulation, as BRD4 facilitates the recruitment of transcriptional machinery to specific gene promoters, thereby influencing cell growth and differentiation. BRD4′s ability to bind to mitotic chromosomes underscores BRD4′s importance in cell cycle progression, particularly in preventing the transition from the G1 phase to the S phase by interacting with the largest subunit of replication factor C, which inhibits DNA elongation. Dysregulation of BRD4 has been implicated in various aggressive cancers, highlighting BRD4′s potential as a therapeutic target in oncology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BRD4 Antibody (C-2) References:
- Hijacking the E3 Ubiquitin Ligase Cereblon to Efficiently Target BRD4. | Lu, J., et al. 2015. Chem Biol. 22: 755-63. PMID: 26051217
- BRD4 Connects Enhancer Remodeling to Senescence Immune Surveillance. | Tasdemir, N., et al. 2016. Cancer Discov. 6: 612-29. PMID: 27099234
- Dynamic Chromatin Targeting of BRD4 Stimulates Cardiac Fibroblast Activation. | Stratton, MS., et al. 2019. Circ Res. 125: 662-677. PMID: 31409188
- Potent BRD4 inhibitor suppresses cancer cell-macrophage interaction. | Yin, M., et al. 2020. Nat Commun. 11: 1833. PMID: 32286255
- Autophagy enhanced by curcumin ameliorates inflammation in atherogenesis via the TFEB-P300-BRD4 axis. | Li, X., et al. 2022. Acta Pharm Sin B. 12: 2280-2299. PMID: 35646539
- YAP/BRD4-controlled ROR1 promotes tumor-initiating cells and hyperproliferation in pancreatic cancer. | Yamazaki, M., et al. 2023. EMBO J. 42: e112614. PMID: 37096681
- The BRD4-NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma. | Durall, RT., et al. 2023. Cancer Res. 83: 3846-3860. PMID: 37819236
- Unlocking the secrets of aging: Epigenetic reader BRD4 as the target to combatting aging-related diseases. | Sun, J., et al. 2024. J Adv Res. 63: 207-218. PMID: 37956861
- Super-enhancers and the super-enhancer reader BRD4: tumorigenic factors and therapeutic targets. | Qian, H., et al. 2023. Cell Death Discov. 9: 470. PMID: 38135679
- KDM5C-Mediated Recruitment of BRD4 to Chromatin Regulates Enhancer Activation and BET Inhibitor Sensitivity. | Qiang, Y., et al. 2024. Cancer Res. 84: 1252-1269. PMID: 38285760
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRD4 Antibody (C-2) | sc-518158 | 200 µg/ml | $322.00 | |||
BRD4 Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-550666 | 200 µg Ab; 40 µg BP | $361.00 | |||
BRD4 Antibody (C-2) AC | sc-518158 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BRD4 Antibody (C-2) HRP | sc-518158 HRP | 200 µg/ml | $322.00 | |||
BRD4 Antibody (C-2) FITC | sc-518158 FITC | 200 µg/ml | $336.00 | |||
BRD4 Antibody (C-2) PE | sc-518158 PE | 200 µg/ml | $349.00 | |||
BRD4 Antibody (C-2) Alexa Fluor® 488 | sc-518158 AF488 | 200 µg/ml | $364.00 | |||
BRD4 Antibody (C-2) Alexa Fluor® 546 | sc-518158 AF546 | 200 µg/ml | $364.00 | |||
BRD4 Antibody (C-2) Alexa Fluor® 594 | sc-518158 AF594 | 200 µg/ml | $364.00 | |||
BRD4 Antibody (C-2) Alexa Fluor® 647 | sc-518158 AF647 | 200 µg/ml | $364.00 | |||
BRD4 Antibody (C-2) Alexa Fluor® 680 | sc-518158 AF680 | 200 µg/ml | $364.00 | |||
BRD4 Antibody (C-2) Alexa Fluor® 790 | sc-518158 AF790 | 200 µg/ml | $364.00 |