BACE Antibody (A-12): sc-365948

BACE Antibody (A-12) is a mouse monoclonal IgG2b antibody that detects BACE in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). BACE, or beta-site amyloid precursor protein cleaving enzyme, plays a crucial role in the pathogenesis of Alzheimer′s disease by cleaving the amyloid precursor protein (APP) to produce amyloid beta-peptide (Aβ), which aggregates to form amyloid plaques, a hallmark of the disease. BACE localization within the Golgi apparatus and endosomes is particularly important, as these cellular compartments facilitate APP processing, leading to Aβ generation. Understanding BACE cellular location is vital for developing therapeutic strategies aimed at inhibiting BACE activity, thereby potentially reducing Aβ production and mitigating the progression of Alzheimer′s disease. Anti-BACE antibody (A-12) serves as an essential tool for researchers investigating the molecular mechanisms underlying Alzheimer′s disease and for exploring potential interventions targeting this critical enzyme.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BACE Antibody (A-12) References:

1. Functional characterization of the 5' flanking region of the BACE gene: identification of a 91 bp fragment involved in basal level of BACE promoter expression.  |  Ge, YW., et al. 2004. FASEB J. 18: 1037-9. PMID: 15059977
2. Flavonols and flavones as BACE-1 inhibitors: structure-activity relationship in cell-free, cell-based and in silico studies reveal novel pharmacophore features.  |  Shimmyo, Y., et al. 2008. Biochim Biophys Acta. 1780: 819-25. PMID: 18295609
3. A new specific BACE-1 inhibitor from the stembark extract of Vitis vinifera.  |  Choi, YH., et al. 2009. Planta Med. 75: 537-40. PMID: 19184970
4. Progress in the development of nonpeptidomimetic BACE 1 inhibitors for Alzheimer's disease.  |  Huang, WH., et al. 2009. Curr Med Chem. 16: 1806-20. PMID: 19442147
5. In vitro BACE-1 inhibitory activity of resveratrol oligomers from the seed extract of Paeonia lactiflora.  |  Choi, CW., et al. 2011. Planta Med. 77: 374-6. PMID: 20890809
6. BACE-1 inhibition prevents the γ-secretase inhibitor evoked Aβ rise in human neuroblastoma SH-SY5Y cells.  |  Jämsä, A., et al. 2011. J Biomed Sci. 18: 76. PMID: 22018341
7. PPAR-α agonist regulates amyloid-β generation via inhibiting BACE-1 activity in human neuroblastoma SH-SY5Y cells transfected with APPswe gene.  |  Zhang, H., et al. 2015. Mol Cell Biochem. 408: 37-46. PMID: 26092426
8. Sevoflurane-Induced Endoplasmic Reticulum Stress Contributes to Neuroapoptosis and BACE-1 Expression in the Developing Brain: The Role of eIF2α.  |  Liu, B., et al. 2017. Neurotox Res. 31: 218-229. PMID: 27682474
9. BACE-1 and γ-Secretase as Therapeutic Targets for Alzheimer's Disease.  |  Maia, MA. and Sousa, E. 2019. Pharmaceuticals (Basel). 12: PMID: 30893882
10. BACE-1 and ADAM-10 as Potential Peripheral Biomarkers for Alzheimer's Disease.  |  Cervellati, C., et al. 2025. Curr Pharm Des.. PMID: 39781737

The preferred BACE antibody is BACE Antibody (61-3E7): sc-33711.