SCAPER Activators
SCAPER activators pertain to a specialized category of chemical compounds that interact with the S-phase kinase-associated protein 2 (Skp2) E3 ubiquitin ligase SCF complex-associated protein, expanding to SCAPER, which is a regulatory protein involved in cell cycle progression. The primary role of SCAPER is to act as a substrate recognition component in the Skp1-Cul1-F-box protein (SCF) ubiquitin ligase complex, which is crucial for ubiquitination - a process that tags proteins for degradation by the proteasome, thereby maintaining cellular homeostasis. SCAPER activators modulate this process by influencing the activity of SCAPER, which, in turn, affects the ubiquitination and subsequent degradation of various protein substrates. The SCF complex, including SCAPER, plays an integral role in the transition between different phases of the cell cycle by targeting specific cell cycle regulators for ubiquitination, and thereby, proteasomal degradation.
The molecular architecture of SCAPER activators is designed to engage with the SCAPER protein, often at specific binding sites, to exert their modulatory effects. This interaction can lead to a conformational change in the SCAPER protein, potentially enhancing its ability to participate in the ubiquitin-proteasome pathway. By modulating the function of SCAPER, these activators can influence the stability and abundance of certain proteins that are integral to the cell cycle. The significance of this lies in the tightly regulated process of protein turnover, which is essential for the normal progression of the cell cycle and for ensuring the fidelity of cellular division and function. Through this mechanism, SCAPER activators contribute to the fine-tuning of cellular proliferation processes, by impacting the ubiquitination and degradation of target proteins within the SCF complex pathway. The ability to modulate such pathways with small molecules is reflective of the intricate balance that exists within cellular systems, where protein levels are precisely controlled to maintain normal physiological functions.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly activates adenylyl cyclase, leading to an increase in cyclic AMP (cAMP) levels. Elevated cAMP activates protein kinase A (PKA), which can phosphorylate SCAPER, potentially enhancing its role in cell cycle progression and facilitating its interaction with other cell cycle regulators. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $97.00 $224.00 | 30 | |
8-Bromo-cAMP is a cAMP analog that activates PKA. Activation of PKA can lead to the phosphorylation of SCAPER, thereby possibly enhancing its activity in the regulation of the cell cycle. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $159.00 $315.00 $598.00 | 34 | |
Isobutylmethylxanthine (IBMX) is a non-selective inhibitor of phosphodiesterases which increases intracellular cAMP levels by preventing its breakdown. Higher cAMP levels lead to PKA activation, which could enhance SCAPER phosphorylation and activity in cell cycle control. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Calcium-dependent protein kinases, such as CaMK, could then phosphorylate SCAPER, affecting its function in the regulation of the cell cycle and possibly enhancing its activity. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin is a SERCA pump inhibitor that leads to increased cytosolic calcium levels. The resultant activation of calcium-dependent signaling pathways could indirectly enhance SCAPER's activity in cell cycle regulation by modulating its phosphorylation state. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA is an activator of protein kinase C (PKC). PKC-mediated phosphorylation pathways could potentially phosphorylate and enhance SCAPER activity, which is involved in cell cycle regulation. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels of cellular proteins. This can indirectly result in the enhanced phosphorylation and activation of SCAPER, as phosphatase inhibition can affect cell cycle progression. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A, like okadaic acid, is an inhibitor of protein phosphatases, particularly PP1 and PP2A. By inhibiting phosphatase activity and thus increasing phosphorylation levels, SCAPER's phosphorylation state may be indirectly enhanced, which is critical for its function in cell cycle regulation. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG has been shown to inhibit multiple protein kinases, thereby altering signaling pathways. Although it has broad activity, it could selectively allow for enhanced SCAPER activity through altering the balance of kinases and phosphatases that modulate SCAPER's role in cell cycle control. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that activates stress-activated protein kinases (SAPKs). Activation of SAPKs can lead to phosphorylation events that may enhance SCAPER's activity in cell cycle progression due to its involvement in regulatory processes. | ||||||