OTUD7A Inhibitors
OTUD7A inhibitors are a category of chemical compounds that interact with the OTUD7A enzyme, a member of the ovarian tumor (OTU) family of deubiquitinating enzymes (DUBs). The OTUD7A enzyme, also known as OTU domain-containing protein 7A, plays a critical role in the intricate ubiquitin-proteasome system (UPS). The UPS is a cellular mechanism responsible for protein degradation and turnover, as well as the regulation of various proteins that are crucial for diverse cellular processes. By mediating the removal of ubiquitin from specific protein substrates, OTUD7A modulates the fate of these proteins, influencing their stability, localization, and activity. OTUD7A inhibitors, therefore, act by selectively binding to this enzyme and impeding its deubiquitinating activity, which in turn affects the ubiquitin-dependent signaling pathways.
The chemical scaffolds of OTUD7A inhibitors are typically designed to mimic the transition state or the substrate of the enzyme, allowing them to bind with high affinity and specificity to the active site of OTUD7A. This inhibition can alter the dynamics of ubiquitination, thereby impacting various cellular functions controlled by ubiquitin signaling. These inhibitors are complex molecules that often require careful design and optimization to ensure that they are effective in engaging with OTUD7A without affecting other DUBs, which could lead to off-target effects. The discovery and development of OTUD7A inhibitors involve a combination of computational modeling, and biochemistry to fine-tune their interaction with the enzyme. As a result, these inhibitors are valuable tools for probing the biological functions of OTUD7A and elucidating the molecular pathways in which it is involved.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG132 is a potent, reversible, and cell-permeable proteasome inhibitor. By inhibiting the proteasome, it prevents the degradation of ubiquitinated proteins. Since OTUD7A is a deubiquitinating enzyme, the inhibition of proteasomal degradation can indirectly decrease the functional activity of OTUD7A by reducing its substrate availability. | ||||||
Lactacystin | 133343-34-7 | sc-3575 sc-3575A | 200 µg 1 mg | $165.00 $575.00 | 60 | |
Lactacystin is an irreversible proteasome inhibitor that selectively binds to and inhibits the 20S proteasome. Similar to MG132, lactacystin's mechanism of action leads to the accumulation of ubiquitinated proteins, potentially reducing the functional activity of OTUD7A by saturating its substrate pool. | ||||||
PR 619 | 2645-32-1 | sc-476324 sc-476324A sc-476324B | 1 mg 5 mg 25 mg | $75.00 $184.00 $423.00 | 1 | |
PR-619 is a broad-spectrum deubiquitinating enzyme inhibitor that can inhibit the activity of OTUD7A directly. By preventing OTUD7A from cleaving ubiquitin from its substrates, PR-619 reduces the ability of OTUD7A to regulate protein stability and signaling pathways. | ||||||
N-Ethylmaleimide | 128-53-0 | sc-202719A sc-202719 sc-202719B sc-202719C sc-202719D | 1 g 5 g 25 g 100 g 250 g | $22.00 $68.00 $210.00 $780.00 $1880.00 | 19 | |
N-Ethylmaleimide is a sulfhydryl-reactive alkylating agent that irreversibly inhibits enzymes with cysteine active sites. As OTUD7A requires a cysteine residue for its deubiquitinating activity, N-Ethylmaleimide can directly inhibit OTUD7A by modifying this crucial cysteine. | ||||||
WP1130 | 856243-80-6 | sc-364650 sc-364650A | 10 mg 50 mg | $480.00 $1455.00 | 1 | |
WP1130 is a selective deubiquitinase inhibitor that indirectly affects OTUD7A by inhibiting other deubiquitinases, leading to an alteration in the overall ubiquitin landscape and potentially diminishing the role of OTUD7A in cellular processes. | ||||||
IU1 | 314245-33-5 | sc-361215 sc-361215A sc-361215B | 10 mg 50 mg 100 mg | $138.00 $607.00 $866.00 | 2 | |
IU1 is an inhibitor that specifically targets UCHL5, a deubiquitinating enzyme. By inhibiting UCHL5, IU1 can alter the ubiquitin homeostasis in the cell and indirectly affect the functional activity of OTUD7A. | ||||||
NSC697923 | 343351-67-7 | sc-391107 sc-391107A | 1 mg 5 mg | $15.00 $51.00 | 3 | |
NSC697923 is a small molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A. By inhibiting this enzyme complex, NSC697923 can decrease the ubiquitination levels of certain substrates, indirectly affecting the activity of OTUD7A by modulating its substrate pool. | ||||||
Ubiquitin E1 Inhibitor, PYR-41 | 418805-02-4 | sc-358737 | 25 mg | $360.00 | 4 | |
PYR-41 is a selective inhibitor of ubiquitin-activating enzyme E1. By preventing the initial step of ubiquitin conjugation, PYR-41 indirectly decreases the functional activity of OTUD7A by limiting the ubiquitination of substrates. | ||||||
MLN 4924 | 905579-51-3 | sc-484814 | 1 mg | $280.00 | 1 | |
MLN4924 is an inhibitor of NEDD8-activating enzyme. Although it primarily reduces neddylation, by disrupting the NEDD8 pathway, it can influence ubiquitination processes and indirectly affect the activity of OTUD7A. | ||||||