Keratin 71 Inhibitors
Chemical inhibitors of Keratin 71 can employ various mechanisms to disrupt the normal function and structure of the protein. Dimethyl sulfoxide (DMSO) can interfere with Keratin 71 by altering its protein-protein interactions, which are essential for maintaining keratin's structural integrity. This alteration mainly occurs through the disruption of hydrophobic interactions within the protein. Formaldehyde, on the other hand, can form cross-links within Keratin 71, causing aggregation or distortion of the protein structure, which leads to a loss of function. Similarly, acrylamide can inhibit Keratin 71 by covalently bonding with cysteine residues, which are crucial for the formation of keratin filaments. This bonding effectively prevents Keratin 71 from assembling into its correct filament structure.
Furthermore, iodoacetamide and N-Ethylmaleimide (NEM) specifically target the thiol groups of cysteine residues in Keratin 71, preventing the formation of disulfide bonds that are necessary for filament stability and, consequently, inhibit the protein's function. Phenylarsine oxide and cadmium chloride also bind to cysteine residues, but they do so by interacting with the vicinal thiol groups, leading to similar inhibitory outcomes. Lead(II) acetate works in a related manner, binding to sulfhydryl groups and disrupting the protein's disulfide bonds that are essential for its filamentous assembly. Sodium dodecyl sulfate (SDS) acts as an anionic detergent, denaturing Keratin 71 and preventing it from forming proper filaments. Organic solvents like methanol and ethanol perturb the hydrophobic interactions within Keratin 71, which are essential for maintaining its structure and function. Lastly, urea disrupts hydrogen bonding within the protein, leading to a loss of its secondary and tertiary structure, which is crucial for Keratin 71's biological role. Each chemical, by targeting specific interactions within Keratin 71, can disrupt its normal structure and function, ultimately inhibiting the protein's proper assembly and activity.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Dimethyl Sulfoxide (DMSO) | 67-68-5 | sc-202581 sc-202581A sc-202581B | 100 ml 500 ml 4 L | $31.00 $117.00 $918.00 | 136 | |
Dimethyl sulfoxide (DMSO) can inhibit Keratin 71 by disrupting protein-protein interactions. DMSO is known to interfere with hydrophobic interactions which are crucial for the structural integrity of keratins. By altering the tertiary and quaternary structures of Keratin 71, DMSO can potentially inhibit its function. | ||||||
FCM Fixation buffer (10X) | sc-3622 | 10 ml @ 10X | $62.00 | 16 | ||
Formaldehyde can form cross-links with proteins, including Keratin 71, which may inhibit its function. Cross-linking can distort the protein structure or cause aggregation, leading to a loss of function. | ||||||
Acrylamide Solution, 40% | 79-06-1 | sc-3721 | 1 L | $100.00 | ||
Acrylamide is capable of disrupting the structure of keratin proteins by forming covalent bonds with cysteine residues. For Keratin 71, such modification can lead to improper filament formation and thus inhibit its function. | ||||||
α-Iodoacetamide | 144-48-9 | sc-203320 | 25 g | $255.00 | 1 | |
Iodoacetamide reacts with cysteine residues in proteins. By alkylating the cysteine residues in Keratin 71, iodoacetamide can inhibit disulfide bond formation essential for keratin filament stability and function. | ||||||
N-Ethylmaleimide | 128-53-0 | sc-202719A sc-202719 sc-202719B sc-202719C sc-202719D | 1 g 5 g 25 g 100 g 250 g | $22.00 $69.00 $214.00 $796.00 $1918.00 | 19 | |
N-Ethylmaleimide (NEM) can inhibit Keratin 71 by irreversibly modifying the thiol groups of cysteine residues, preventing disulfide bond formation that is necessary for keratin filament stability. | ||||||
Phenylarsine oxide | 637-03-6 | sc-3521 | 250 mg | $41.00 | 4 | |
Phenylarsine oxide binds to vicinal thiol groups in proteins. Binding to the cysteine residues in Keratin 71 can inhibit its function by disrupting disulfide bond formation which is essential for filament stability. | ||||||
Cadmium chloride, anhydrous | 10108-64-2 | sc-252533 sc-252533A sc-252533B | 10 g 50 g 500 g | $56.00 $183.00 $352.00 | 1 | |
Cadmium chloride can inhibit Keratin 71 by binding to cysteine residues, which are important for disulfide bond formation and keratin filament assembly and stability. | ||||||
Lead(II) Acetate | 301-04-2 | sc-507473 | 5 g | $85.00 | ||
Lead(II) acetate can bind to sulfhydryl groups of cysteine residues in Keratin 71, inhibiting its proper function and filament formation by disrupting disulfide bonds. | ||||||
Sodium dodecyl sulfate | 151-21-3 | sc-264510 sc-264510A sc-264510B sc-264510C | 25 g 100 g 500 g 1 kg | $78.00 $119.00 $419.00 $603.00 | 11 | |
SDS is an anionic detergent that can denature keratin proteins including Keratin 71, leading to disruption of protein structure and inhibition of filament formation. | ||||||
Urea | 57-13-6 | sc-29114 sc-29114A sc-29114B | 1 kg 2 kg 5 kg | $31.00 $43.00 $78.00 | 17 | |
Urea can denature proteins by disrupting hydrogen bonds. For Keratin 71, urea can lead to loss of secondary and tertiary structure which is essential for its function. | ||||||