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Acrylamide Solution, 40% (CAS 79-06-1)

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Application:
Acrylamide Solution, 40% is commonly used matrix for electrophoretic separation of nucleic acids and proteins
CAS Number:
79-06-1
Molecular Weight:
71.08
Molecular Formula:
C3H5ON
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Acrylamide solution serves as a monomer for the polymerization of polyacrylamide gels, which are widely used in techniques such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) for separating proteins based on their molecular weight. In this process, acrylamide monomers polymerize in the presence of a cross-linking agent, such as N,N′-methylenebisacrylamide (MBAA), forming a porous gel matrix that allows the separation of biomolecules. Moreover, acrylamide solution is utilized in the synthesis of polyacrylamide-based hydrogels, which find applications in drug delivery, tissue engineering, and biomedical research. These hydrogels can absorb and retain large amounts of water, mimicking the properties of biological tissues and providing a scaffold for cell growth and tissue regeneration. Additionally, acrylamide solution is employed in the synthesis of polyacrylamide-based polymers used in various industrial processes, including wastewater treatment, papermaking, and enhanced oil recovery. Its ability to form stable polymers with desirable properties, such as high water solubility and biocompatibility, makes it a valuable component in these applications. Furthermore, acrylamide solution is utilized in biochemical assays and research experiments for its ability to modify and cross-link proteins, nucleic acids, and other biomolecules. Its chemical reactivity enables the functionalization of biomolecules and the formation of covalent bonds, facilitating the study of protein-protein interactions, enzyme kinetics, and biomolecular structure-function relationships.


Acrylamide Solution, 40% (CAS 79-06-1) References

  1. On-line stacking techniques for the nonaqueous capillary electrophoretic determination of acrylamide in processed food.  |  Tezcan, F. and Erim, FB. 2008. Anal Chim Acta. 617: 196-9. PMID: 18486658
  2. Acetic Acid-urea polyacrylamide gel electrophoresis of proteins.  |  Smith, BJ. 1984. Methods Mol Biol. 1: 63-73. PMID: 20512675
  3. Polyacrylamide Gel Electrophoresis.  |  Green, MR. and Sambrook, J. 2020. Cold Spring Harb Protoc. 2020: PMID: 33262236
  4. Polyacrylamide gel electrophoresis: reaction of acrylamide at alkaline pH with buffer components and proteins.  |  Geisthardt, D. and Kruppa, J. 1987. Anal Biochem. 160: 184-91. PMID: 3565751
  5. Preparative acrylamide electrophoresis: a single gel system.  |  Gordon, AH. and Louis, LN. 1967. Anal Biochem. 21: 190-200. PMID: 6082097
  6. Nondenaturing polyacrylamide gel electrophoresis of proteins.  |  Walker, JM. 1994. Methods Mol Biol. 32: 17-22. PMID: 7524940
  7. Acetic acid-urea polyacrylamide gel electrophoresis of proteins.  |  Smith, BJ. 1994. Methods Mol Biol. 32: 39-47. PMID: 7951739
  8. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.  |  Weber, K., et al. 1972. Methods in enzymology. Vol. 26 Academic Press: 3-27.
  9. Denaturing polyacrylamide gel electrophoresis.  |  Albright, et al. 2000. Current Protocols in Nucleic Acid Chemistry. 1: A-3B.
  10. Neutral polyacrylamide gel electrophoresis.  |  Sambrook, et al. 2006. Cold Spring Harbor Protocols. 2006.1: pdb-prot4028.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Acrylamide Solution, 40%, 1 L

sc-3721
1 L
$98.00