I-Plastin Activators
I-Plastin Activators encompass a range of chemical compounds that specifically enhance the functional activity of I-Plastin through distinct biochemical pathways. Forskolin, Phorbol 12-myristate 13-acetate (PMA), and Isoproterenol serve as activators by increasing the cellular levels of cAMP, leading to PKA activation. PKA, in turn, phosphorylates I-Plastin, thereby enhancing its functionality in stabilizing actin filaments and cellular structures. Similarly, Epinephrine and Dibutyryl-cAMP also activate the PKA pathway, providing additional routes forI-Plastin Activators to phosphorylate and activate I-Plastin. Ionomycin, by raising intracellular calcium, indirectly activates calmodulin-dependent kinase II, which may phosphorylate I-Plastin, thus facilitating its role in actin cross-linking. Meanwhile, SNAP, through its release of nitric oxide, activates guanylate cyclase, leading to PKG-mediated phosphorylation of I-Plastin, which is pivotal for cytoskeletal rearrangements.
The functional enhancement of I-Plastin by these activators is a critical aspect of cellular cytoskeletal organization, particularly in microvilli formation and cell-cell junction stabilization. Calyculin A inhibits phosphatases that typically dephosphorylate proteins, thereby indirectly maintaining I-Plastin in a phosphorylated, active state. This ensures sustained actin binding and stabilization, integral for proper cellular structure and motility. Anisomycin and Bisindolylmaleimide I, although operating through different pathways-JNK activation and PKC inhibition, respectively-converge on enhancing the phosphorylation state of I-Plastin. Rolipram, by inhibiting PDE4, indirectly sustains cAMP levels and PKA activity, again linking back to the phosphorylation and activation of I-Plastin.
SEE ALSO...
Items 1 to 10 of 12 total
Display:
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin directly stimulates adenylate cyclase, resulting in an increase of cyclic AMP (cAMP) levels in cells. Elevated cAMP activates protein kinase A (PKA), which can then phosphorylate target proteins, including I-Plastin, leading to its functional enhancement within the cellular cytoskeletal organization. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is a diacylglycerol analog which activates protein kinase C (PKC). PKC can phosphorylate I-Plastin, which is a prerequisite for its activity in actin bundling within cells, thus enhancing its functionality in stabilizing cellular structures. | ||||||
Ionomycin, free acid | 56092-81-0 | sc-263405 sc-263405A | 1 mg 5 mg | $96.00 $264.00 | 2 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate calmodulin-dependent kinase II (CaMKII). CaMKII then potentially phosphorylates I-Plastin, facilitating its role in actin cross-linking and stabilization of microvilli. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
Isoproterenol is a beta-adrenergic agonist that can raise intracellular cAMP levels, thereby activating PKA. PKA activation can lead to the phosphorylation and subsequent activation of I-Plastin, enhancing its role in actin filament organization. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $41.00 $104.00 $201.00 $1774.00 $16500.00 | ||
Epinephrine interacts with adrenergic receptors, which can stimulate cAMP production and PKA activation. PKA can phosphorylate I-Plastin, enhancing its actin-binding function and stabilizing cell-cell junctions. | ||||||
(±)-S-Nitroso-N-acetylpenicillamine | 79032-48-7 | sc-200319B sc-200319 sc-200319A | 10 mg 20 mg 100 mg | $74.00 $114.00 $374.00 | 18 | |
SNAP releases nitric oxide (NO), which can activate guanylate cyclase and increase cyclic GMP (cGMP) levels. cGMP-dependent protein kinase (PKG) might phosphorylate I-Plastin, leading to its enhanced activity in cytoskeletal rearrangements. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $108.00 $169.00 $295.00 $561.00 $835.00 | 2 | |
8-Bromo-cAMP is a cell-permeable analog of cAMP that activates PKA. Activated PKA can enhance I-Plastin activity by phosphorylation, which is important for its role in actin filament bundling and cellular motility. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a JNK activator that can lead to the activation of c-Jun, which may enhance the expression of proteins involved in cytoskeletal dynamics. Through this pathway, I-Plastin function in actin bundling could be enhanced. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation levels of proteins. This can result in enhanced activity of I-Plastin through sustained phosphorylation, impacting actin binding and stabilization. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cAMP analog that activates PKA. PKA then can phosphorylate I-Plastin, leading to enhanced activity in actin cytoskeleton organization and microvilli formation. | ||||||