His-probe Activators
His-probe Activators refers to a group of chemicals that, when used in conjunction with HisProbe-HRP, can enhance the detection of His-tagged proteins in Western blots and microplates. These activators aim to optimize the conditions for nickel coordination with histidine tags and influence the microenvironment surrounding HisProbe-HRP. Imidazole, a heterocyclic compound, can coordinate with nickel ions, improving the interaction between nickel and histidine tags. Nickel sulfate serves as a source of nickel ions, contributing to the activation of HisProbe-HRP and enhancing the specificity of His-tagged protein detection. 1,10-Phenanthroline, as a chelating agent, may impact the binding of nickel ions to histidine tags, influencing the efficiency of detection.
L-Histidine, one of the amino acids containing a histidine residue, may influence the coordination between nickel ions and histidine tags. Triton X-100, a non-ionic surfactant, can modulate the microenvironment around HisProbe-HRP, optimizing nickel coordination. Sodium phosphate serves as a buffering agent to maintain stable conditions for efficient His-tagged protein detection. N-Ethylmaleimide (NEM) and sodium azide play roles in modulating thiol chemistry and peroxidase activity, respectively, within the microenvironment of HisProbe-HRP. Their inclusion requires careful consideration to balance the effects on histidine tags and peroxidase activity, ensuring optimal conditions for detection. In summary, the His-probe activators represent a set of chemicals that can be strategically employed to enhance the sensitivity and efficiency of His-tagged protein detection using HisProbe-HRP. By carefully adjusting the concentrations of these activators, researchers can optimize the conditions for nickel coordination with histidine tags, thereby improving the overall performance of the detection assay.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Imidazole | 288-32-4 | sc-204776 sc-204776A sc-204776B sc-204776C | 25 g 100 g 1 kg 5 kg | $27.00 $56.00 $84.00 $343.00 | 2 | |
Imidazole is a heterocyclic compound that can coordinate with nickel ions. In the context of HisProbe-HRP, imidazole may enhance the interaction between nickel ions and histidine tags, potentially improving the binding efficiency of His-tagged proteins to the detection reagent. | ||||||
Nickel Sulfate | 7786-81-4 | sc-507407 | 5 g | $63.00 | ||
Nickel sulfate is a source of nickel ions. Adding nickel sulfate may contribute to the activation of HisProbe-HRP by providing additional nickel ions for coordination with histidine residues. This can enhance the sensitivity and specificity of His-tagged protein detection in Western blots and microplates. | ||||||
1,10-Phenanthroline | 66-71-7 | sc-255888 sc-255888A | 2.5 g 5 g | $23.00 $32.00 | ||
1,10-Phenanthroline is a chelating agent that forms stable complexes with metal ions. In the context of HisProbe-HRP, it may influence the binding of nickel ions to histidine tags, potentially affecting the efficiency of His-tagged protein detection. | ||||||
L-Histidine | 71-00-1 | sc-394101 sc-394101A sc-394101B sc-394101C sc-394101D | 25 g 100 g 250 g 500 g 1 kg | $54.00 $84.00 $189.00 $204.00 $339.00 | 1 | |
L-Histidine is one of the amino acids containing a histidine residue. Its presence may influence the coordination between nickel ions and histidine tags in HisProbe-HRP. The addition of L-histidine could potentially modulate the sensitivity of His-tagged protein detection by affecting the binding interactions between the histidine tag and nickel ions. | ||||||
Triton X-100 | 9002-93-1 | sc-29112 sc-29112A | 100 ml 500 ml | $20.00 $42.00 | 55 | |
Triton X-100 is a non-ionic surfactant. Its inclusion may impact the microenvironment surrounding HisProbe-HRP, potentially influencing the efficiency of nickel coordination and interaction with histidine tags. This surfactant could play a role in optimizing the conditions for His-tagged protein detection in Western blots and microplates. | ||||||
Sodium Phosphate, Dibasic | 7558-79-4 | sc-203277 sc-203277A sc-203277D sc-203277B sc-203277C | 500 g 1 kg 2 kg 5 kg 10 kg | $57.00 $176.00 $239.00 $380.00 $588.00 | 11 | |
Sodium phosphate is a buffering agent that may influence the overall conditions of the assay involving HisProbe-HRP. Proper buffering can help maintain the stability of nickel ions and optimize the reaction environment for efficient His-tagged protein detection. | ||||||
N-Ethylmaleimide | 128-53-0 | sc-202719A sc-202719 sc-202719B sc-202719C sc-202719D | 1 g 5 g 25 g 100 g 250 g | $22.00 $69.00 $214.00 $796.00 $1918.00 | 19 | |
N-Ethylmaleimide is a thiol-reactive compound. Its inclusion may modulate the thiol chemistry within the microenvironment of HisProbe-HRP, potentially influencing the stability or reactivity of the histidine tags on proteins. The presence of NEM could be considered in optimizing conditions for enhancing the detection efficiency of His-tagged proteins. | ||||||
Sodium azide | 26628-22-8 | sc-208393 sc-208393B sc-208393C sc-208393D sc-208393A | 25 g 250 g 1 kg 2.5 kg 100 g | $43.00 $155.00 $393.00 $862.00 $90.00 | 8 | |
Sodium azide is often used as an inhibitor of peroxidase activity. In the context of HisProbe-HRP, the addition of sodium azide may influence the enzymatic activity of horseradish peroxidase, potentially affecting the detection process. Careful consideration of sodium azide concentration is important to balance its inhibitory effect on peroxidase activity while maintaining efficient His-tagged protein detection. | ||||||