GnT-II Inhibitors
Chemical inhibitors of GnT-II target various stages of the glycosylation pathway to achieve functional inhibition of the protein. Swainsonine is one such inhibitor that interferes with the alpha-mannosidase II enzyme, crucial for the formation of complex N-glycans. By impeding the function of this enzyme, Swainsonine effectively results in suboptimal glycosylation of proteins, including GnT-II, thereby curtailing its activity. Similarly, Kifunensine and Deoxymannojirimycin act as inhibitors of alpha-mannosidase I, leading to an accumulation of high-mannose glycans. This accumulation indirectly impedes the activity of GnT-II by hampering the maturation of N-glycans to a form that is a prerequisite for the function of GnT-II. Deoxynojirimycin and Castanospermine further contribute to this inhibition by targeting alpha-glucosidases I and II and glucosidase, respectively, which are responsible for trimming glucose residues from N-linked glycans. Their inhibition results in a disruption of proper glycan assembly, which is essential for the action of GnT-II.
Bromoconduritol, Celgosivir, Nojirimycin, and 1-Deoxynojirimycin add to the arsenal of GnT-II inhibitors by their actions on beta-glucosidases and alpha-glucosidases, respectively. The inhibition of these enzymes leads to improper glycan processing, which is imperative for the correct folding and functioning of GnT-II substrates. Miglustat indirectly inhibits GnT-II by altering the composition of glycosphingolipids within cellular membranes, thereby potentially influencing the membrane environment and the activity of membrane-associated enzymes like GnT-II. Salbostatin, by inhibiting trehalose-6-phosphate phosphatase, induces changes in the cellular sugar phosphate pools, which can affect the glycosylation reactions that GnT-II is involved in. Lastly, Isofagomine's inhibition of beta-glucosidase can result in the improper processing of N-glycans, which is a necessary step for the functional activity of GnT-II, thus establishing another mechanism through which the activity of GnT-II can be indirectly inhibited.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Swainsonine | 72741-87-8 | sc-201362 sc-201362C sc-201362A sc-201362D sc-201362B | 1 mg 2 mg 5 mg 10 mg 25 mg | $138.00 $251.00 $631.00 $815.00 $1832.00 | 6 | |
Swainsonine is an inhibitor of alpha-mannosidase II, which is necessary for the proper formation of N-glycans. By inhibiting this enzyme, the production of complex N-glycans is reduced, which may result in improper glycosylation of proteins, including GnT-II, thereby functionally inhibiting its activity. | ||||||
Kifunensine | 109944-15-2 | sc-201364 sc-201364A sc-201364B sc-201364C | 1 mg 5 mg 10 mg 100 mg | $135.00 $540.00 $1025.00 $6248.00 | 25 | |
Kifunensine is a mannosidase I inhibitor which leads to the accumulation of high-mannose glycans. This accumulation can indirectly inhibit GnT-II by preventing the progression of N-glycans to the form that GnT-II can act on, thus inhibiting its function. | ||||||
Deoxymannojirimycin hydrochloride | 84444-90-6 | sc-201360 sc-201360A | 1 mg 5 mg | $93.00 $239.00 | 2 | |
Deoxymannojirimycin is an inhibitor of alpha-mannosidase I, similar to Kifunensine. It can lead to the accumulation of unprocessed high-mannose N-glycans, thereby indirectly inhibiting GnT-II by hindering the substrate availability for GnT-II action. | ||||||
Deoxynojirimycin | 19130-96-2 | sc-201369 sc-201369A | 1 mg 5 mg | $73.00 $145.00 | ||
Deoxynojirimycin inhibits alpha-glucosidases I and II, enzymes that are responsible for trimming glucose residues from N-linked glycans. This inhibition can lead to the misfolding of glycoproteins and thus indirectly inhibit the function of GnT-II by affecting the substrate availability and proper folding required for its activity. | ||||||
Castanospermine | 79831-76-8 | sc-201358 sc-201358A | 100 mg 500 mg | $184.00 $632.00 | 10 | |
Castanospermine is a glucosidase inhibitor that can prevent the proper trimming and processing of N-glycans, which is a prerequisite for GnT-II activity. By inhibiting this process, castanospermine can indirectly inhibit the function of GnT-II by limiting the maturation of its glycoprotein substrates. | ||||||
Celgosivir | 121104-96-9 | sc-488385 sc-488385A sc-488385B | 5 mg 25 mg 100 mg | $525.00 $902.00 $2700.00 | ||
Celgosivir inhibits alpha-glucosidase I, which is necessary for the initial glycan processing in the ER. This inhibition can result in the accumulation of misfolded glycoproteins, indirectly inhibiting GnT-II by affecting the proper formation of substrates on which GnT-II acts. | ||||||
Isofagomine D-Tartrate | 957230-65-8 | sc-207767 sc-207767A sc-207767C sc-207767B | 5 mg 10 mg 50 mg 25 mg | $387.00 $724.00 $2015.00 $1223.00 | ||
Isofagomine is an inhibitor of beta-glucosidase. By inhibiting the glucosidase activity, the proper processing and maturation of N-glycans could be hindered, which in turn can indirectly inhibit the function of GnT-II in the glycosylation pathway. | ||||||