CARF Inhibitors
Chemical inhibitors of CARF can impede its function through various mechanisms related to its role in DNA damage response and cell cycle regulation. Staurosporine, a potent kinase inhibitor, can inhibit kinases that are involved in phosphorylating CARF, thus potentially reducing CARF's activity in signaling pathways. Similarly, UCN-01's inhibition of protein kinase C can disrupt signaling pathways that CARF is part of, leading to a reduction in its functional activity. Cisplatin and Mitomycin C, both DNA crosslinkers, can overwhelm the DNA repair capacity of CARF by inducing extensive DNA damage, thereby inhibiting its repair function. Etoposide and Camptothecin, which stabilize DNA-topoisomerase complexes, result in increased DNA breaks. This can saturate CARF's ability to mediate DNA repair, effectively inhibiting its function.
On the other hand, Hydroxyurea and Aphidicolin target DNA synthesis and replication. Hydroxyurea reduces DNA synthesis by inhibiting ribonucleotide reductase, which can indirectly inhibit CARF by decreasing the replication stress that CARF normally addresses. Aphidicolin's inhibition of DNA polymerases α and δ leads to stalled replication forks, which could compromise CARF's involvement in the replication process. Actinomycin D inhibits RNA polymerase, potentially affecting transcription-coupled DNA repair processes where CARF might be involved. Betulinic acid induces apoptosis via mitochondrial pathways, which can inhibit CARF's role in cell survival and cycle regulation. MG-132 disrupts the ubiquitin-proteasome system, which can indirectly inhibit CARF's role in stress responses. Mevinolin, by inhibiting HMG-CoA reductase, affects cholesterol biosynthesis and cell membrane composition, which can indirectly influence CARF's interactions within the cell and its functional response to cellular stress.
Items 1 to 10 of 11 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $150.00 $388.00 | 113 | |
Staurosporine is a potent kinase inhibitor that can inhibit a wide range of PKs. Given CARF's role in DNA damage response which may involve kinase signaling, staurosporine could inhibit kinases that phosphorylate CARF, thereby diminishing CARF's functional activity. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $76.00 $216.00 | 101 | |
Cisplatin forms DNA adducts and crosslinks, which could inhibit CARF by overwhelming its ability to mediate DNA repair processes. Overactivation of DNA damage may lead to the sequestration of CARF, impeding its function. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $32.00 $170.00 $385.00 | 63 | |
Etoposide stabilizes the DNA-topoisomerase II complex, causing DNA breaks. CARF is implicated in the DNA damage response, and the increased DNA breaks could saturate CARF's function in repair mechanisms. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $57.00 $182.00 $92.00 | 21 | |
Camptothecin inhibits DNA topoisomerase I, leading to DNA breaks. CARF's role in DNA repair implies that the excess of DNA breaks would inhibit the functional capacity of CARF to adequately respond to DNA damage. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $76.00 $255.00 | 18 | |
Hydroxyurea inhibits ribonucleotide reductase, leading to decreased DNA synthesis. This could indirectly inhibit CARF by reducing the replication stress under which CARF normally functions during DNA repair. | ||||||
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $82.00 $300.00 $1082.00 | 30 | |
Aphidicolin is a specific inhibitor of DNA polymerase α and δ, leading to inhibition of DNA replication. CARF's involvement in the replication process could be compromised due to the stalling of replication forks. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $73.00 $238.00 $717.00 $2522.00 $21420.00 | 53 | |
Actinomycin D binds to DNA and inhibits RNA polymerase, which could lead to the inhibition of transcription-coupled DNA repair, a process in which CARF could be involved, thus inhibiting its function. | ||||||
Mitomycin C | 50-07-7 | sc-3514A sc-3514 sc-3514B | 2 mg 5 mg 10 mg | $65.00 $99.00 $140.00 | 85 | |
Mitomycin C is a DNA crosslinker that can lead to extensive DNA damage, potentially overwhelming CARF's DNA repair capacity and thereby inhibiting its function. | ||||||
Betulinic Acid | 472-15-1 | sc-200132 sc-200132A | 25 mg 100 mg | $115.00 $337.00 | 3 | |
Betulinic acid can induce apoptosis through pathways that involve mitochondrial membrane alterations. Since CARF is known to influence cell survival, the induction of apoptosis could inhibit CARF's role in cell cycle regulation. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG-132 is a proteasome inhibitor that can lead to the accumulation of polyubiquitinated proteins, potentially affecting the ubiquitin-proteasome system. CARF is associated with the cellular response to stress, which may be inhibited by the disruption of proteasome function. | ||||||