1700021F02Rik Activators
1700021F02Rik Activators encompass a diverse array of chemical compounds that indirectly augment the functional activity of 1700021F02Rik through various signaling pathways. Forskolin and IBMX operate through the elevation of intracellular cyclic nucleotides, cAMP and cGMP, which activate PKA and other kinases, leading to phosphorylation events that can enhance the activity of 1700021F02Rik. Epigallocatechin gallate and Genistein inhibit specific kinases. 1700021F02Rik Activators represent a specific group of chemical compounds that have the capacity to enhance the expression of the gene designated as 1700021F02Rik in mice. This gene, a unique sequence within the mouse genome, is responsible for the coding of a particular protein or RNA molecule. The activators in this category are characterized by their ability to upregulate the gene's expression, which results in increased levels of its gene product. The discovery and confirmation of these activators involve a meticulous process that begins with high-throughput screening. This technique allows researchers to screen a multitude of compounds to identify those that can cause an upsurge in the expression of the 1700021F02Rik gene.
Following the initial screening, compounds that show promise as activators undergo a rigorous validation process. Quantitative PCR (qPCR) is employed to measure the levels of 1700021F02Rik mRNA in cells that contain the compounds, providing a direct assessment of their impact on gene transcription. This is complemented by Western blot analysis, which is used to detect the protein coded by 1700021F02Rik. This technique can confirm that the elevated mRNA levels result in an increase in protein synthesis. It also allows for the observation of any changes in protein stability or post-translational modifications that may occur due to the presence of the activator compounds. These methods collectively provide a multifaceted view of the effect of 1700021F02Rik Activators on both the gene and its protein product, offering insights into the magnitude and nature of gene expression modulation.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
IBMX is a nonspecific inhibitor of phosphodiesterases, which prevents the breakdown of cAMP and cGMP. The resultant accumulation of cAMP/cGMP can activate PKA and other cAMP/cGMP-dependent pathways, thereby indirectly increasing the functional activity of 1700021F02Rik. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is a potent activator of protein kinase C (PKC), which is involved in multiple signaling pathways. Activation of PKC can lead to the phosphorylation of proteins that interact with or regulate the function of 1700021F02Rik, potentially enhancing its activity. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
Epigallocatechin gallate is a polyphenol that can inhibit certain types of kinases. The inhibition of these kinases can remove negative regulatory influences on pathways in which 1700021F02Rik is involved, thus indirectly enhancing its activity. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 is an ionophore that increases intracellular calcium levels. The elevation in calcium can activate calcium-dependent signaling pathways, which could include those that lead to the activation of 1700021F02Rik, thus enhancing its functional activity. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a specific inhibitor of PI3K, which alters the PI3K/AKT pathway. This modulation can result in changes in downstream signaling that may favor the functional activity of 1700021F02Rik through indirect mechanisms. | ||||||
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $67.00 $223.00 $425.00 | 97 | |
Wortmannin is another PI3K inhibitor that can affect the PI3K/AKT pathway, similar to LY294002. This disruption can lead to altered signaling that potentially enhances the activity of 1700021F02Rik indirectly. | ||||||
D-erythro-Sphingosine-1-phosphate | 26993-30-6 | sc-201383 sc-201383D sc-201383A sc-201383B sc-201383C | 1 mg 2 mg 5 mg 10 mg 25 mg | $165.00 $322.00 $570.00 $907.00 $1727.00 | 7 | |
Sphingosine-1-phosphate is a lipid signaling molecule that activates sphingosine-1-phosphate receptors. This can lead to the activation of pathways that modulate the function of proteins including 1700021F02Rik, thereby enhancing its activity. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $90.00 $349.00 | 284 | |
SB203580 is a specific inhibitor of p38 MAP kinase. By inhibiting p38, it can redirect signaling through alternative pathways, which may include those that enhance the activity of 1700021F02Rik. | ||||||
Genistein | 446-72-0 | sc-3515 sc-3515A sc-3515B sc-3515C sc-3515D sc-3515E sc-3515F | 100 mg 500 mg 1 g 5 g 10 g 25 g 100 g | $45.00 $164.00 $200.00 $402.00 $575.00 $981.00 $2031.00 | 46 | |
Genistein is a tyrosine kinase inhibitor and by inhibiting these kinases, it may reduce competitive signaling pathways and indirectly enhance pathways where 1700021F02Rik is functional. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $153.00 $396.00 | 113 | |
Staurosporine is a broad-spectrum protein kinase inhibitor. Despite its wide range of targets, it can preferentially influence signaling pathways that interact with or regulate 1700021F02Rik, leading to an enhancement of its activity through indirect mechanisms. | ||||||