1700020N15Rik Activators
The designation 1700020N15Rik Activators describes a category of chemical agents that are identified by their ability to increase the activity of the gene known as 1700020N15Rik in the mouse genome. This gene is a sequence of DNA that encodes a specific protein or RNA molecule, and the activators in this class function to upregulate its expression, leading to a higher production of the gene's product. The initial identification of these activators typically involves high-throughput screening, where a large library of compounds is tested for their ability to enhance the activity of 1700020N15Rik. Techniques such as luciferase reporter assays are often used in these screens. In such an assay, the 1700020N15Rik promoter is attached to a luciferase reporter gene, and the activation of this promoter by a chemical compound results in the production of light, which can be measured quantitatively.
After promising candidates are identified through high-throughput screens, they proceed to secondary validation using more precise and gene-specific assays. Quantitative PCR (qPCR) is a critical tool for assessing the impact of these activators on the mRNA levels of 1700020N15Rik. This method quantifies the amount of mRNA present, and when applied to cells that contain the activator compounds, it can confirm whether there is an upregulation of the 1700020N15Rik gene at the transcriptional level. Complementary to qPCR, Western blot analysis is applied to detect and quantify the protein product. This method can confirm that the increased mRNA levels lead to a corresponding increase in protein production. It can also identify if the protein undergoes any changes in stability or post-translational modifications in the presence of the activators. These techniques collectively offer insights not just into the quantity of gene expression but also the quality of the protein product and its functional state.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
EGCG is a polyphenol that can inhibit certain protein kinases. By inhibiting these kinases, EGCG could shift the balance of cellular signaling pathways, thus potentially enhancing the activity of 1700020N15Rik by reducing negative regulatory inputs. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), and PKC-mediated signaling pathways often have numerous downstream effects, including the activation of proteins like 1700020N15Rik through phosphorylation or other regulatory mechanisms. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, leading to the activation of calcium-dependent signaling pathways. This increase in calcium signaling could enhance the activity of 1700020N15Rik if it is regulated by calcium-sensitive pathways or proteins. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a PI3K inhibitor. By inhibiting PI3K, the compound could disinhibit pathways that are negatively regulated by PI3K signaling, potentially leading to the activation of 1700020N15Rik through a relief of inhibition. | ||||||
SB 203580 | 152121-47-6 | sc-3533 sc-3533A | 1 mg 5 mg | $90.00 $349.00 | 284 | |
SB203580 is a specific inhibitor of p38 MAPK. Inhibition of p38 MAPK can shift cellular signaling towards alternative pathways, potentially leading to the activation of proteins such as 1700020N15Rik if they are part of these alternative pathways. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 is a calcium ionophore like Ionomycin, and it similarly increases intracellular calcium levels. Elevated calcium can activate proteins and pathways that respond to calcium signals, potentially enhancing the activity of 1700020N15Rik. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $105.00 $250.00 | 8 | |
Zaprinast inhibits PDEs, primarily PDE5, leading to increased cGMP levels similar to Sildenafil. This can enhance PKG signaling pathways, which could result in the activation of 1700020N15Rik if it is regulated by cGMP or PKG-dependent pathways. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $77.00 $216.00 | 18 | |
Rolipram is a selective inhibitor of PDE4, leading to increased cAMP levels. By elevating cAMP, it activates PKA and could potentially enhance the functional activity of 1700020N15Rik through cAMP-dependent signaling pathways. | ||||||
Y-27632, free base | 146986-50-7 | sc-3536 sc-3536A | 5 mg 50 mg | $186.00 $707.00 | 88 | |
Y-27632 is a ROCK inhibitor, which leads to the inhibition of the RhoA/ROCK pathway. This inhibition can result in the activation of alternative signaling pathways that may include the upregulation of the functional activity of 1700020N15Rik. | ||||||