Date published: 2025-9-6

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Wnt-4 抗体 (B-6): sc-376279

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说明书
  • Wnt-4 抗体 B-6 是小鼠单克隆 IgG1 κ,Wnt-4抗体, 在23篇文献中引用,规格为200 µg/ml
  • 免疫mouse物种的Wnt-4的氨基酸1-70
  • Wnt-4 抗体 (B-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Wnt-4; 也和以下物种反应,包括: porcine
  • 抗Wnt-4抗体(B-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Wnt-4 (B-6): sc-376279免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 Wnt-4 抗体 (B-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Wnt-4 抗体 (B-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Wnt-4抗体(B-6)是一种IgG1 κ小鼠单克隆Wnt-4抗体(也称为WNT4抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Wnt-4蛋白。Wnt-4抗体(B-6)既有非偶联型抗Wnt-4抗体形式,也有多种偶联型抗Wnt-4抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。高度保守的Wnt基因家族的产品,包括Wnt-1到Wnt-10,在调节细胞生长和分化方面发挥关键作用。Wnt-1是一种富含半胱氨酸的分泌型糖蛋白,与细胞膜相关,可能作为细胞粘附的关键调节因子。Wnt-1对胚胎神经系统的正常发育至关重要,当在乳腺组织中表达不当时,会导致增生和肿瘤进展。Wnt-3也参与肿瘤发生,而Wnt-2和Wnt-4可能与人类乳腺组织的异常增殖有关。Wnt-1、Wnt-3和Wnt-10b已与FGF-3一起被证实在小鼠乳腺肿瘤病毒诱导的小鼠乳腺癌的发展中起作用。已证实Wnt家族成员在体内与Sonic hedgehog(Shh)相互作用,诱导体节组织中的肌生成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Wnt-4 抗体 (B-6) 参考文献:

  1. Wnt 基因  |  Nusse, R. and Varmus, HE. 1992. Cell. 69: 1073-87. PMID: 1617723
  2. 人肾上腺皮质肿瘤和培养的肾上腺细胞中 WNT-4 mRNA 的表达。  |  Kuulasmaa, T., et al. 2008. Horm Metab Res. 40: 668-73. PMID: 18553255
  3. Wnt-4和Wnt-11在维甲酸诱导的神经元分化过程中发挥不同作用  |  Elizalde, C., et al. 2011. Stem Cells. 29: 141-53. PMID: 21280163
  4. Exendin-4能上调胰腺β细胞增殖的新型调节因子Wnt-4的表达。  |  Heller, C., et al. 2011. Am J Physiol Endocrinol Metab. 301: E864-72. PMID: 21771967
  5. 确定受 Wnt-4 调控的基因,Wnt-4 是卵巢承诺的关键信号。  |  Naillat, F., et al. 2015. Exp Cell Res. 332: 163-78. PMID: 25645944
  6. 透明细胞肾癌中 Wnt-4 蛋白的免疫组化表达  |  Pavlovic, O., et al. 2021. J Clin Med. 10: PMID: 34945091
  7. 音速刺猬和 Wnt 家族成员的联合信号在体节中诱导成肌性 bHLH 基因的表达。  |  Münsterberg, AE., et al. 1995. Genes Dev. 9: 2911-22. PMID: 7498788
  8. 对小鼠 Wnt 蛋白的生化分析表明,它们既有共同的特性,也有不同的特性。  |  Burrus, LW. and McMahon, AP. 1995. Exp Cell Res. 220: 363-73. PMID: 7556445
  9. Wnt 基因对乳腺上皮细胞的不同转化。  |  Wong, GT., et al. 1994. Mol Cell Biol. 14: 6278-86. PMID: 8065359
  10. 人类 Wnt 基因 2, 3, 4 和 7B 在人类乳腺细胞系以及正常和疾病状态的人类乳腺组织中的差异表达。  |  Huguet, EL., et al. 1994. Cancer Res. 54: 2615-21. PMID: 8168088
  11. Wnt-1 蛋白能与细胞表面结合的特性。  |  Schryver, B., et al. 1996. Oncogene. 13: 333-42. PMID: 8710372
  12. 小鼠乳腺肿瘤中 MMTV 诱导的突变:它们与人类乳腺癌的潜在相关性。  |  Callahan, R. 1996. Breast Cancer Res Treat. 39: 33-44. PMID: 8738604

订购信息

产品名称产品编号规格价格数量收藏夹

Wnt-4 抗体 (B-6)

sc-376279
200 µg/ml
$316.00

Wnt-4 (B-6): m-IgG1 BP-HRP 套装

sc-531716
200 µg Ab; 20 µg BP
$354.00

Wnt-4 抗体 (B-6) AC

sc-376279 AC
500 µg/ml, 25% agarose
$416.00

Wnt-4 抗体 (B-6) HRP

sc-376279 HRP
200 µg/ml
$316.00

Wnt-4 抗体 (B-6) FITC

sc-376279 FITC
200 µg/ml
$330.00

Wnt-4 抗体 (B-6) PE

sc-376279 PE
200 µg/ml
$343.00

Wnt-4 抗体 (B-6) Alexa Fluor® 488

sc-376279 AF488
200 µg/ml
$357.00

Wnt-4 抗体 (B-6) Alexa Fluor® 546

sc-376279 AF546
200 µg/ml
$357.00

Wnt-4 抗体 (B-6) Alexa Fluor® 594

sc-376279 AF594
200 µg/ml
$357.00

Wnt-4 抗体 (B-6) Alexa Fluor® 647

sc-376279 AF647
200 µg/ml
$357.00

Wnt-4 抗体 (B-6) Alexa Fluor® 680

sc-376279 AF680
200 µg/ml
$357.00

Wnt-4 抗体 (B-6) Alexa Fluor® 790

sc-376279 AF790
200 µg/ml
$357.00

Could I have an aliquot of FREE 10 µg sample of Wnt-4 (B-6): sc-376279?

Asked by: Russoa
Thank you for your question. We would be happy to send you a sample of this antibody! Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com, and we will gladly assist you!
Answered by: Tech Service
Date published: 2017-06-28

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-02-02
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from PERFECTThis antibody is one of the best that I ever used.
Date published: 2017-12-21
Rated 5 out of 5 by from works very well with mouse epithelial cellsI tested free trial antibody with mouse epithelial cells and could get clear single band for Wnt-4 protein by western blot. Antibody can be diluted by 1:1000 ratio and generate good result too.
Date published: 2017-10-18
Rated 5 out of 5 by from WBThis antibody worked very well for western blot. 1:500.
Date published: 2017-09-19
Rated 5 out of 5 by from IHCWnt-4 used for IHC staining rat spleen tissue section, diluted 1:200. Worked perfect and binding only Wnt-4 (as supposed).
Date published: 2017-09-09
Rated 5 out of 5 by from Wnt-4 IHCchick embryo, PFA fixed, cryosectioned 2nd Ab Alexa fluor488
Date published: 2017-09-05
Rated 4 out of 5 by from Worked GreatBlocked in 5% BSA-TBST 1:800. Incubated in primary overnight in cold room. Tested on Murine Fallopian Tube Tissue and Human Ovarian Cell Lines. Strong bands at 43 kD, but there are a lot of background as well.
Date published: 2017-07-24
Rated 5 out of 5 by from WBThis antibody was used at 1/800 dilution in TBST ,nice strong band seen ,when test mouse spleen tissue,it shows positive band.
Date published: 2017-07-08
Rated 5 out of 5 by from western blotUsed many suppliers of wnt4 antibody, santa 's this antibody is more economy but high effency, i used for wb, good signal.
Date published: 2017-06-11
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Wnt-4 抗体 (B-6) is rated 4.7 out of 5 by 13.
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