Date published: 2025-9-6

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VPS4B 抗体 (A-11): sc-377162

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说明书
  • VPS4B 抗体 A-11 是小鼠单克隆 IgG2a κ,VPS4B抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的VPS4B的C-terminus附近的氨基酸355-387之间
  • VPS4B 抗体 (A-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 VPS4B
  • 抗VPS4B抗体(A-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VPS4B (A-11): sc-377162免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是VPS4B Antibody (A-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与VPS4B Antibody (A-11) 打包提供(请参阅下面的订购信息)。

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関連項目

VPS4B抗体(A-11)是一种IgG2a κ小鼠单克隆VPS4B抗体(也称为MIG1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的VPS4B蛋白。VPS4B抗体(A-11)既可作为非共轭抗VPS4B抗体形式提供,也可作为多种共轭形式的抗VPS4B抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。E类液泡蛋白分选(vps)蛋白对于酵母内吞途径中受体的适当分选是必需的。酵母Vps4p是AAA蛋白家族(与多种细胞活动相关的ATP酶)的成员,并在将蛋白质从液泡前内体区室中运输出来方面发挥重要作用。在人类中,已知酵母Vps4p的两个非等位直系同源蛋白(VPS4A和VPS4B),并且可以彼此形成异聚体复合物。VPS4(在小鼠中也称为SKD1)蛋白都是E类VPS,并涉及细胞内蛋白质运输,类似于酵母中的Vps4p。与人类CHMP1蛋白有关的多泡体形成,与VPS4存在物理相互作用。HIV-1使用细胞机制从受感染的细胞中出芽,并且需要VPS4和TSG101/VPS23来进行此出芽过程。VPS4的显性负突变体抑制液泡蛋白分选,并且还阻止HIV-1和MLV出芽。因此,逆转录病毒通常在出芽过程中使用VPS途径来形成多泡体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VPS4B 抗体 (A-11) 参考文献:

  1. TSG101/哺乳动物 VPS23 和哺乳动物 VPS28 直接相互作用,并被招募到 VPS4 诱导的内体。  |  Bishop, N. and Woodman, P. 2001. J Biol Chem. 276: 11735-42. PMID: 11134028
  2. CHMP1 是一个新定义的囊泡贩运蛋白家族的成员。  |  Howard, TL., et al. 2001. J Cell Sci. 114: 2395-404. PMID: 11559748
  3. 哺乳动物细胞表达两种 VPS4 蛋白,它们都参与细胞内蛋白质的运输。  |  Scheuring, S., et al. 2001. J Mol Biol. 312: 469-80. PMID: 11563910
  4. Tsg101和液泡蛋白分拣途径对HIV-1的萌发至关重要。  |  Garrus, JE., et al. 2001. Cell. 107: 55-65. PMID: 11595185
  5. 抵抗是徒劳的:HIV-1 对细胞机制的同化。  |  Perez, OD. and Nolan, GP. 2001. Immunity. 15: 687-90. PMID: 11728331

订购信息

产品名称产品编号规格价格数量收藏夹

VPS4B 抗体 (A-11)

sc-377162
200 µg/ml
$316.00

VPS4B (A-11): m-IgG Fc BP-HRP 套装

sc-526086
200 µg Ab; 10 µg BP
$354.00

VPS4B (A-11): m-IgG2a BP-HRP 套装

sc-552052
200 µg Ab; 10 µg BP
$354.00

VPS4B 抗体 (A-11) AC

sc-377162 AC
500 µg/ml, 25% agarose
$416.00

VPS4B 抗体 (A-11) HRP

sc-377162 HRP
200 µg/ml
$316.00

VPS4B 抗体 (A-11) FITC

sc-377162 FITC
200 µg/ml
$330.00

VPS4B 抗体 (A-11) PE

sc-377162 PE
200 µg/ml
$343.00

VPS4B 抗体 (A-11) Alexa Fluor® 488

sc-377162 AF488
200 µg/ml
$357.00

VPS4B 抗体 (A-11) Alexa Fluor® 546

sc-377162 AF546
200 µg/ml
$357.00

VPS4B 抗体 (A-11) Alexa Fluor® 594

sc-377162 AF594
200 µg/ml
$357.00

VPS4B 抗体 (A-11) Alexa Fluor® 647

sc-377162 AF647
200 µg/ml
$357.00

VPS4B 抗体 (A-11) Alexa Fluor® 680

sc-377162 AF680
200 µg/ml
$357.00

VPS4B 抗体 (A-11) Alexa Fluor® 790

sc-377162 AF790
200 µg/ml
$357.00

VPS4B (A-11) 中和勝肽

sc-377162 P
100 µg/0.5 ml
$68.00

Hi, I would like to know if it is possible to have the following antibody without BSA or any protein: Anti-VPS4B Antibody (A-11): sc-377162 ? Thank you, Aline

Asked by: Aline Zbinden
Thank you for your question. We can offer this antibody in custom formulations as a special order product. Please contact our technical service team directly for a quote.
Answered by: Tech Support Europe
Date published: 2022-09-05

What application is the blocking peptide sc-377162 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Vps4 A11 mouse monoclonalWorks very well, detects both isoforms. Used at 1 ug/ml.
Date published: 2017-04-17
Rated 5 out of 5 by from Great antibodyThe VPS4B Antibody (A-11): sc-377162 works very well for Western blot showing clear band with minimal background.
Date published: 2017-01-19
Rated 5 out of 5 by from worked well in WBI applied this antibody a month ago, ant I used it in WB. The cell of my experiment is the vascular smooth muscle from SD RAT. I can see the clear result.We can repeat the result three times.
Date published: 2017-01-18
Rated 5 out of 5 by from Produced positive Western blot data of VPS4BProduced positive Western blot data of VPS4B expression in MIA PaCa-2, K-562, Hep G2 and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-12-02
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VPS4B 抗体 (A-11) is rated 5.0 out of 5 by 4.
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