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Vimentin 抗体 (J144): sc-53464

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说明书
  • Vimentin 抗体 J144 是小鼠单克隆 IgM, 在31篇文献中引用,提供 200 µg/ml
  • 针对human来源的横纹肌肉瘤细胞系JR1
  • 推荐用于 mouse, rat 和 human 来源的Vimentin WB, IP, IF 和 IHC(P)检测
  • 看 Vimentin (E-5): sc-373717,可查看有标记的 Vimentin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Vimentin 抗体 (J144)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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Vimentin 抗体(J144)是一种小鼠IgM单克隆抗体,免疫原信息为人源性横纹肌肉瘤细胞系JR1。该抗体对Vimentin具有高度特异性,Vimentin是一种主要在间充质来源细胞中表达的关键中间丝蛋白。Vimentin对于维持细胞完整性、提供机械支撑以及促进细胞迁移等过程至关重要,特别是在上皮细胞向间充质细胞转化(EMT)和肿瘤转移期间。重要的是,Vimentin经历多种翻译后修饰,包括磷酸化、乙酰化和泛素化,以调节其功能。在细胞分裂过程中,Vimentin在Ser38和Ser55等位点的磷酸化至关重要,它可调节丝状物的分解和重组,从而实现正常的细胞分裂。Vimentin的乙酰化会影响其稳定性和丝状物的组装,从而影响细胞对压力的反应。另一方面,泛素化作用可导致Vimentin降解,从而影响细胞在动态环境中的形态和流动性。Vimentin还与肌动蛋白等关键蛋白相互作用,为肌肉细胞提供结构完整性,并与β-catenin相互作用,影响细胞粘附和与肿瘤发生相关的信号通路。克隆号为J144的抗体可用于石蜡包埋切片免疫组织化学(IHC)、免疫荧光(IF)、免疫沉淀(IP)和蛋白免疫印迹(WB)应用,为癌症研究、EMT和细胞骨架动力学研究提供了广泛的用途。该抗体有非偶联形式和偶联形式,偶联形式包括HRP和FITC,可在各种实验中广泛使用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Vimentin 抗体 (J144) 参考文献:

  1. 波形蛋白中间丝的几何控制。  |  Shabbir, SH., et al. 2014. Biomaterials. 35: 1359-66. PMID: 24268665
  2. 波形蛋白介导 C3 外酶的吸收。  |  Rohrbeck, A., et al. 2014. PLoS One. 9: e101071. PMID: 24967582
  3. 波形蛋白保护分化中的干细胞免受压力。  |  Pattabiraman, S., et al. 2020. Sci Rep. 10: 19525. PMID: 33177544
  4. 瓜氨酸化波形蛋白介导肺纤维化的发生和发展。  |  Li, FJ., et al. 2021. Sci Transl Med. 13: PMID: 33731433
  5. 冠状动脉疾病中的变构蛋白靶向。  |  Liu, H., et al. 2021. Int J Cardiol. 339: 144. PMID: 34252499
  6. Vimentin中间丝介导粘弹性基质上的细胞形态。  |  Swoger, M., et al. 2022. ACS Appl Bio Mater. 5: 552-561. PMID: 34995457
  7. 波形蛋白抑制小鼠肠道炎症和肿瘤发生。  |  Wang, L., et al. 2022. Front Cell Dev Biol. 10: 862237. PMID: 35399505
  8. 纤维蛋白形成中细胞外纤维蛋白原和纤维蛋白原之间的新型相互作用。  |  Martinez-Vargas, M., et al. 2023. Thromb Res. 221: 97-104. PMID: 36495717
  9. 细胞骨架中的波形蛋白在细菌感染和宿主防御中的不同作用。  |  Miao, C., et al. 2023. J Cell Sci. 136: PMID: 36594661
  10. 波形蛋白通过增强中心体功能和微管乙酰化支持细胞极化。  |  Saldanha, R., et al. 2023. bioRxiv.. PMID: 36824848

订购信息

产品名称产品编号规格价格数量收藏夹

Vimentin 抗体 (J144)

sc-53464
200 µg/ml
$322.00

Are there any issues with using sc-53464: Vimentin (J144) mouse monoclonal primary antibody on mouse samples?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53464 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by jenniferc from Very good antibodyVimentin Antibody (J144) worked well by Western blot. This antibody produced strong and specific band
Date published: 2017-01-31
Rated 4 out of 5 by Mehdi from HCT116 CRC and SW480 CRC cellsHCT116 CRC and SW480 CRC cells - more antibody gave better signals
Date published: 2016-01-21
Rated 5 out of 5 by SF from Publishable data for use in IF in human cervicalPublishable data for use in IF in human cervical fibroblast. -SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by SH from Publishable data for use in WBPublishable data for use in WB, IF, and IHC in human lung adenocarcinoma. -SCBT Publication Review
Date published: 2015-01-02
Rated 5 out of 5 by JT from Strong specific band in WB in SJRH30 andStrong specific band in WB in SJRH30 and RD whole cell lysates. -SCBT QC
Date published: 2014-09-27
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Vimentin 抗体 (J144) is rated 4.8 out of 5 by 5.
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