VCP抗体 (5) 推荐用于 mouse, rat, human 和 porcine 来源的VCP WB, IP, IF 和 IHC(P)检测

VCP 抗体 (5): sc-57492

VCP抗体 (5) is rated 4.8 out of 5 by 10.
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说明书

    • 抗-VCP 抗体 5 是小鼠单克隆 IgG2b (kappa轻链),VCP抗体,规格为200 µg/ml
    • 免疫human物种的VCP的氨基酸792-806
    • 抗-VCP 抗体 (5) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human 和porcine 来源的 VCP
    • 抗-VCP 抗体 (5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取VCP (5): sc-57492免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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I am using VCP (5): sc-57492 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect for ICC Funciona excelentemente para ICC de fibroblastos primarios usandolo hasta 1:500 en PBST (0.1% Triton) + 10% Serum 1h RT u O/N 4ºC.
Date published: 2020-02-19
Rated 5 out of 5 by from Works for both IF and Western blot Brought this antibody 6 months ago. It works for both western blot and IF staining very well.
Date published: 2019-09-05
Rated 5 out of 5 by from Best VCP Antibody ever! Very clean and very specific! We use it to detect endogenous protein and it works great at 1:1000 loading very little lysate (10ug or less)
Date published: 2018-01-31
Rated 5 out of 5 by from Works very well on mouse liver WB Works very well on mouse liver lysates western blotting. very clean bands.
Date published: 2017-10-10
Rated 5 out of 5 by from Great for WB! Consistent, clean and strong bands seen across multiple whole cell lysate samples in Western Blot.
Date published: 2017-01-23
Rated 5 out of 5 by from good Abs this Abs was good for IP both on human and mouse cells
Date published: 2016-11-11
Rated 5 out of 5 by from Excellent Antibody I used this antibody on my Westerns and it worked very well.
Date published: 2016-10-31
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-05-13
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