Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
U2AF65 Antibody (MC3) - Western Blotting - Image 360292
U2AF65 抗体 (MC3): sc-53942. 对 HeLa (A) 和 HEK293 (B) 全细胞裂解液以及 Jurkat (C) 和 SK-N-MC (D) 核提取物中 U2AF65 的表达进行 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
U2AF65 抗体 (MC3): sc-53942. 对 HeLa (A) 和 HEK293 (B) 全细胞裂解液以及 Jurkat (C) 和 SK-N-MC (D) 核提取物中 U2AF65 的表达进行 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

U2AF65 抗体 (MC3): sc-53942

4.8(13)
寫評論提問

说明书
  • U2AF65 抗体 MC3 是小鼠单克隆 IgG2b κ，U2AF65抗体, 在31篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组U2AF65
  • U2AF65 抗体 (MC3) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的U2AF65
  • 抗U2AF65抗体（MC3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取U2AF65 (MC3): sc-53942的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 U2AF65 抗体 (MC3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与U2AF65 抗体 (MC3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

U2AF65 抗体（MC3）是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学方法检测小鼠、大鼠和人源的 U2AF65 蛋白。抗 U2AF65 抗体 (MC3) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。U2AF65 在前 mRNA 的剪接过程中起着至关重要的作用，它是 U2 辅助因子复合物的一部分，而 U2 辅助因子复合物对剪接体的组装至关重要。U2AF65 位于细胞核中，与前-mRNA 及其他剪接体成分相互作用，促进剪接过程中对分支点序列的识别。U2AF65 的正确定位可确保高效准确的剪接，这对于生成成熟的 mRNA 和调控基因表达至关重要。U2AF65 与 U2AF35 形成一个异源二聚体，这些蛋白一起对 U2 小核核糖核蛋白（snRNP）招募到剪接位点起着不可或缺的作用，突出了 U2AF65 在 mRNA 成熟的复杂过程中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

U2AF65 抗体 (MC3) 参考文献:

  1. 生化和电子显微镜联合分析揭示了哺乳动物 U2 snRNP 的结构。  |  Krämer, A., et al. 1999. J Cell Biol. 145: 1355-68. PMID: 10385517
  2. 含有剪接体蛋白 SAPs 49, 130, 145 和 155 的蛋白质复合物的特征。  |  Das, BK., et al. 1999. Mol Cell Biol. 19: 6796-802. PMID: 10490618
  3. U2 小核核糖核蛋白辅助因子的鉴定, 纯化和生化特性。  |  Zamore, PD. and Green, MR. 1989. Proc Natl Acad Sci U S A. 86: 9243-7. PMID: 2531895
  4. RBM39-U2AF65 剪接因子复合物中 UHM-ULM 的相互作用。  |  Stepanyuk, GA., et al. 2016. Acta Crystallogr D Struct Biol. 72: 497-511. PMID: 27050129
  5. U2AF65 通过正向调节 mTOR-SREBP-1c 信号通路，促进牛乳腺上皮细胞的乳汁合成和生长。  |  Yu, Y., et al. 2019. Cell Biochem Funct. 37: 93-101. PMID: 30773658
  6. U2AF65 在 SMN 替代剪接中的依赖性 SF3B1 功能  |  Choi, N., et al. 2020. Cells. 9: PMID: 33317029
  7. U2AF65/circNCAPG/RREB1 反馈环通过激活 TGF-β 通路促进胶质瘤干细胞的恶性表型。  |  Li, H., et al. 2023. Cell Death Dis. 14: 23. PMID: 36635261
  8. 果蝇的保守前 mRNA 剪接因子 U2AF：对生存能力的要求  |  Kanaar, R., et al. 1993. Science. 262: 569-73. PMID: 7692602
  9. 预拼接体成分 SAP 49 和 SAP 145 在一个复合体中相互作用，牵连到 U2 snRNP 与分支位点的系链。  |  Champion-Arnaud, P. and Reed, R. 1994. Genes Dev. 8: 1974-83. PMID: 7958871
  10. 体内剪接所需的 U2AF 同源物  |  Potashkin, J., et al. 1993. Science. 262: 573-5. PMID: 8211184
  11. CUS1是冷敏感U2 snRNA突变的抑制因子，是一种与人类SAP 145同源的新型酵母剪接因子。  |  Wells, SE., et al. 1996. Genes Dev. 10: 220-32. PMID: 8566755
  12. 酵母剪接因子 3b（SF3b）亚基同源物的结构和亚基相互作用的一致性。  |  Igel, H., et al. 1998. RNA. 4: 1-10. PMID: 9436903

订购信息

产品名称产品编号规格价格数量收藏夹

U2AF65 抗体 (MC3)

sc-53942
200 µg/ml
$316.00

U2AF65 (MC3): m-IgG Fc BP-HRP 套装

sc-528589
200 µg Ab; 10 µg BP
$354.00

U2AF65 (MC3): m-IgGκ BP-HRP 套装

sc-521001
200 µg Ab, 40 µg BP
$354.00

U2AF65 抗体 (MC3) AC

sc-53942 AC
500 µg/ml, 25% agarose
$416.00

U2AF65 抗体 (MC3) HRP

sc-53942 HRP
200 µg/ml
$316.00

U2AF65 抗体 (MC3) FITC

sc-53942 FITC
200 µg/ml
$330.00

U2AF65 抗体 (MC3) PE

sc-53942 PE
200 µg/ml
$343.00

U2AF65 抗体 (MC3) Alexa Fluor® 488

sc-53942 AF488
200 µg/ml
$357.00

U2AF65 抗体 (MC3) Alexa Fluor® 546

sc-53942 AF546
200 µg/ml
$357.00

U2AF65 抗体 (MC3) Alexa Fluor® 594

sc-53942 AF594
200 µg/ml
$357.00

U2AF65 抗体 (MC3) Alexa Fluor® 647

sc-53942 AF647
200 µg/ml
$357.00

U2AF65 抗体 (MC3) Alexa Fluor® 680

sc-53942 AF680
200 µg/ml
$357.00

U2AF65 抗体 (MC3) Alexa Fluor® 790

sc-53942 AF790
200 µg/ml
$357.00

I am wondering why you do not link this product to the citation of the paper that describes the isolation of this hibridoma (Gama-Carvalho et al 1997) and provides information on the antibody epitope (located between aa 138 and 161). M Gama-Carvalho

Asked by: MGamaC
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-03-23

Hi I would like to know the exact sequence of epitope of this antibody or please let me know if you have peptide I can purchase for this antibody production. Thank you

Asked by: Rnpworld
Thank you for your question. U2AF65 Antibody (MC3): sc-53942 was raised against raised against recombinant U2AF65 of human origin. The epitope of this antibody on its target protein has not been determined yet, so the exact epitope region is not yet known.
Answered by: Tech Support Europe
Date published: 2020-03-01

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 3
Date published: 2017-02-14
  • y_2025, m_8, d_29, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_53942, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 123ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great for WBI used 1: 1000 to do the WB. It provide very strong band at 65kDa.
Date published: 2023-07-11
Rated 5 out of 5 by from Excellent in WBWB in cell lysate of MDA dilution 1:1000 Clear and well detection of a band at the correct molecular weight
Date published: 2019-04-24
Rated 5 out of 5 by from Good for IFGave strong, positive signal in the nucleus (as expected) in PFA-fixed mouse primary neurons. Used at 1:100 for IF.
Date published: 2018-09-26
Rated 5 out of 5 by from Perfect for westernUsed to detect endogenous protein from HEK293T cells. Single band, expected size, RNAi verified.
Date published: 2018-08-02
Rated 5 out of 5 by from usd for WBI detects U2AF65 levels very nicely in WB (dilution 1:800, 5%BSA/TBS/Tween). I have tested it on lysate from mouse kidney total lysate ,very strong band at 65kda.
Date published: 2017-07-29
Rated 5 out of 5 by from Works wellThe U2AF65 antibody works amazingly well in Western blot which gave us single band around 65 kDa in mouse kidney tissue at 40 ug total protein concentration . I found the most optimal dilution for the antibody to be 1:500, however it was still possible to detect bands at further dilution (1:1000).
Date published: 2017-07-29
Rated 5 out of 5 by from Very good for IFI used 1: 2000 to do the IF. Very specific and strong staining!!!
Date published: 2017-07-27
Rated 5 out of 5 by from Target bandThis is a a sensitive and specific antibody for U2AF65 protein analysis,it provide us specific band at 65kDa.
Date published: 2017-07-02
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_53942, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
U2AF65 抗体 (MC3) is rated 4.8 out of 5 by 13.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_53942, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 110ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。