Date published: 2025-9-9
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Tyrosinase Antibody (T311) - Western Blotting - Image 394812
酪氨酸酶抗体 (T311) Alexa Fluor® 488: sc-20035 AF488. Hep G2 (A), Jurkat (B) 和 A-431 (C) 全细胞裂解液中酪氨酸酶表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
酪氨酸酶抗体 (T311) Alexa Fluor® 488: sc-20035 AF488. Hep G2 (A), Jurkat (B) 和 A-431 (C) 全细胞裂解液中酪氨酸酶表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

Tyrosinase 抗体 (T311): sc-20035

4.7(23)
寫評論提問

说明书
  • Tyrosinase 抗体 T311 是小鼠单克隆 IgG2a κ，Tyrosinase抗体, 在102篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组Tyrosinase
  • Tyrosinase 抗体 (T311) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Tyrosinase
  • 抗Tyrosinase抗体（T311）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tyrosinase (T311): sc-20035的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Tyrosinase 抗体 (T311) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Tyrosinase 抗体 (T311) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

酪氨酸酶抗体（T311）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类来源的酪氨酸酶蛋白。酪氨酸酶（T311）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。酪氨酸酶（TYR）是一种I型膜蛋白和含铜酶，在黑色素生成中起着至关重要的作用，而黑色素是脊椎动物的主要色素。黑色素生物合成可保护皮肤和眼睛免受紫外线辐射，酪氨酸酶则催化黑色素生物合成中酪氨酸羟化的关键限速步骤。酪氨酸酶活性不足可导致各种形式的白化病，凸显其在色素沉着中的重要性。酪氨酸酶与相关蛋白TRP1和TRP2相互作用，这些蛋白也在黑素细胞中表达，并参与黑素体中的黑色素合成。这些蛋白质具有相似的跨膜结构，包括两个金属结合区和富含半胱氨酸的表皮生长因子结构域，定位于黑素体膜上。在形成聚集在一起的多酶复合体时，每个蛋白质都有独特的催化活性，有助于黑色素生物聚合物的整体生物合成，其中TRP1在稳定黑素细胞中的酪氨酸酶方面发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tyrosinase 抗体 (T311) 参考文献:

  1. 人类酪氨酸酶催化活性的突变图谱。  |  Tripathi, RK., et al. 1992. J Biol Chem. 267: 23707-12. PMID: 1429711
  2. 第二种与酪氨酸酶相关的蛋白质 TRP-2 是一种黑色素生成酶，被称为 DOPAchrome tautomerase。  |  Tsukamoto, K., et al. 1992. EMBO J. 11: 519-26. PMID: 1537333
  3. 酪氨酸酶激活的黑色素瘤前药。  |  Jawaid, S., et al. 2009. Anticancer Agents Med Chem. 9: 717-27. PMID: 19538169
  4. 人酪氨酸酶和酪氨酸酶相关蛋白的结构和功能。  |  Lai, X., et al. 2018. Chemistry. 24: 47-55. PMID: 29052256
  5. 克隆和表达编码小鼠酪氨酸酶的 cDNA。  |  Shibahara, S., et al. 1986. Nucleic Acids Res. 14: 2413-27. PMID: 3008090
  6. 哺乳动物酪氨酸酶--黑色素细胞色素沉着的关键调节控制点。  |  Hearing, VJ. and Jiménez, M. 1987. Int J Biochem. 19: 1141-7. PMID: 3125075
  7. 酪氨酸酶抑制剂作为潜在的抗菌剂。  |  Yuan, Y., et al. 2020. Eur J Med Chem. 187: 111892. PMID: 31810785
  8. 天然产物作为酪氨酸酶抑制剂。  |  Doğan, A. and Akocak, S. 2024. Enzymes. 56: 85-109. PMID: 39304292
  9. 哺乳动物的酪氨酸酶催化黑色素生物合成过程中的三个反应。  |  Körner, A. and Pawelek, J. 1982. Science. 217: 1163-5. PMID: 6810464
  10. 高分子量形式的酪氨酸酶和酪氨酸酶相关蛋白：黑色素生成复合物的证据。  |  Orlow, SJ., et al. 1994. J Invest Dermatol. 103: 196-201. PMID: 8040609
  11. 人类酪氨酸酶相关蛋白-2 cDNA 的分子特征。黑色素细胞中的表达模式。  |  Bouchard, B., et al. 1994. Eur J Biochem. 219: 127-34. PMID: 8306979
  12. 黑色素生成复合物内的分子相互作用：来自 B16 小鼠黑色素瘤的酪氨酸酶和 TRP1 异二聚体的形成。  |  Jiménez-Cervantes, C., et al. 1998. Biochem Biophys Res Commun. 253: 761-7. PMID: 9918801

订购信息

产品名称产品编号规格价格数量收藏夹

Tyrosinase 抗体 (T311)

sc-20035
200 µg/ml
$316.00

Tyrosinase (T311): m-IgGκ BP-HRP 套装

sc-551064
200 µg Ab; 40 µg BP
$354.00

Tyrosinase 抗体 (T311) AC

sc-20035 AC
500 µg/ml, 25% agarose
$416.00

Tyrosinase 抗体 (T311) HRP

sc-20035 HRP
200 µg/ml
$316.00

Tyrosinase 抗体 (T311) FITC

sc-20035 FITC
200 µg/ml
$330.00

Tyrosinase 抗体 (T311) PE

sc-20035 PE
200 µg/ml
$343.00

Tyrosinase 抗体 (T311) Alexa Fluor® 488

sc-20035 AF488
200 µg/ml
$357.00

Tyrosinase 抗体 (T311) Alexa Fluor® 546

sc-20035 AF546
200 µg/ml
$357.00

Tyrosinase 抗体 (T311) Alexa Fluor® 594

sc-20035 AF594
200 µg/ml
$357.00

Tyrosinase 抗体 (T311) Alexa Fluor® 647

sc-20035 AF647
200 µg/ml
$357.00

Tyrosinase 抗体 (T311) Alexa Fluor® 680

sc-20035 AF680
200 µg/ml
$357.00

Tyrosinase 抗体 (T311) Alexa Fluor® 790

sc-20035 AF790
200 µg/ml
$357.00

Hello, I'm using sc-20035 (Lot #K2420) and noticed a visible band but unclear pattern in WB. Could you share the production date and any validation info for this lot? Any advice on application or dilution would be helpful. Thanks!

Asked by: Hyun Young Shin
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-08-06

Can I try Tyrosinase ab (6A207) sc-73244, instead of T311?, because T311 did not work.

Asked by: taro27
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-07-16

I am wondering whether you could tell me a epitope of this product (Tyrosinase (T311)). N-ter or C-ter is enough to know. Many thanks

Asked by: Anonymous
Thank you for your question. This antibody was raised against recombinant Tyrosinase of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact your local distributor. or technical service.
Answered by: Tech Service
Date published: 2019-07-08

この抗体は、anti-mouse IgG (H+L)-HRPで検出できますか？ 1次抗体1/500、2時抗体1/4000で、全くバンドが出ません。

Asked by: mouse1234
Thank you for your inquiry. Anti-mouse IgG (H+L)-HRP should work with sc-20035, which is a mouse monoclonal IgG2a. For your problem, I would like to recommend you to contact the seller to ask for the after-sales service, we will check more details and do what we can do for you. For more questions, please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-07-14

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-20035 PE) or fluoroscein (sc-20035 FITC), Alexa Fluor® 488 (sc-20035 AF488) or Alexa Fluor® 647 (sc-20035 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Service 11
Date published: 2017-01-10
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_20035, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 8ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good for Western BlotUseful antibody to detect robust tyrosinase expression in melanoma cells.
Date published: 2023-11-30
Rated 5 out of 5 by from Tyrosinase antibodyThe antibody is good on western blot. The band is clear.
Date published: 2023-08-06
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 5 out of 5 by from Works well in western blotGives specific signal in melanotic MNT-1 cells at the expected molecular size
Date published: 2020-09-18
Rated 1 out of 5 by from This antibody cannot recognize tyrosinaseThis antibody cannot recognize tyrosinase protein, becaseu it detects unknown protein in cells that do not express tyrosinase.
Date published: 2019-07-13
Rated 5 out of 5 by from 产品不错,值得信赖因为第一次做免疫组化，不知道用哪种抗体，抱着试一试的心态申请了试用装，实验结果很理想，产品不错，值得信赖。
Date published: 2018-11-27
Rated 5 out of 5 by from Using only a single antibody is so convenient!I've purchased this HRP conjugated many times. It always works great. It increases the efficiency of conducting Western blots in the lab.
Date published: 2018-05-23
Rated 5 out of 5 by from Good sensitivity and specificityTyrosinase (T311) sc-20035 shows a strong signal in Western blot when used for human MNT1 cell line.
Date published: 2018-04-17
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_zh_CN, sid_20035, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
Tyrosinase 抗体 (T311) is rated 4.7 out of 5 by 23.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_zh_CN, sid_20035, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 7ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。