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TSHR Antibody (3B12) - Immunohistochemistry - Image 284754
TSHR 抗体 (3B12): sc-53542. 福尔马林固定, 石蜡包埋的人甲状腺组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
TSHR 抗体 (3B12): sc-53542. 福尔马林固定, 石蜡包埋的人甲状腺组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.

TSHR 抗体 (3B12): sc-53542

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寫評論提問

说明书
  • TSHR 抗体 3B12 是小鼠单克隆 IgG1，TSHR抗体, 在12篇文献中引用，规格为200 µg/ml
  • 抗-TSHR 抗体 (3B12) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat, human, bovine, porcine, feline 和canine 来源的 TSHR
  • TSHR抗体（3B12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TSHR (3B12): sc-53542的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成TSHR 抗体 (3B12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

TSHR 抗体 (3B12) 是一种小鼠单克隆 IgG1 抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和流式细胞术 (FCM) 检测小鼠、大鼠、人、牛、猪、猫和犬的 TSHR 蛋白。TSHR (3B12) 抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。促甲状腺激素受体（TSHR）是一种调节甲状腺激素合成和释放的糖蛋白，通过介导促甲状腺激素（TSH）的作用，在甲状腺功能中发挥着至关重要的作用。TSHR 主要位于甲状腺滤泡细胞的表面，TSHR 在这里启动信号级联，导致甲状腺激素的产生，而甲状腺激素对新陈代谢、生长和发育至关重要。TSHR 的正确定位对其功能至关重要，因为正确的定位可确保 TSH 与 TSHR 有效结合并激活下游信号通路。TSHR 的失调可导致各种甲状腺疾病，包括甲状腺功能亢进症和甲状腺功能减退症，这凸显了 TSHR 在维持内分泌平衡方面的重要性。TSHR 的第三个细胞质环已被确定为调节磷酸肌醇和环磷酸腺苷形成的关键，这进一步强调了 TSHR 影响甲状腺生理的复杂机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TSHR 抗体 (3B12) 参考文献:

  1. 细胞表面表达的促甲状腺激素受体的亚基结构。  |  Tanaka, K., et al. 1999. J Biol Chem. 274: 33979-84. PMID: 10567361
  2. 促甲状腺激素受体突变对单克隆抗体和促甲状腺激素结合及生物活性的影响。  |  Sanders, J., et al. 2006. Thyroid. 16: 1195-206. PMID: 17199429
  3. 一例先天性甲状腺功能减退症患者的促甲状腺激素（甲状腺刺激素）受体基因发生新型突变。  |  Jeziorowska, A., et al. 2006. Thyroid. 16: 1303-9. PMID: 17199441
  4. 技术透视：监测蛋白质-蛋白质相互作用的现代方法揭示了功能性促甲状腺激素受体的寡聚化。  |  Persani, L., et al. 2007. Nat Clin Pract Endocrinol Metab. 3: 180-90. PMID: 17237844
  5. 通过第二代检测法确定抗促甲状腺激素受体抗体。  |  Mankaï, A., et al. 2007. Clin Chem Lab Med. 45: 26-9. PMID: 17243910
  6. 促甲状腺激素受体：促甲状腺激素和促甲状腺激素受体抗体对多聚化、裂解和信号传导的影响。  |  Latif, R., et al. 2009. Endocrinol Metab Clin North Am. 38: 319-41, viii. PMID: 19328414
  7. BANCR 的表达通过靶向促甲状腺激素受体促进甲状腺乳头状癌的发生。  |  Zheng, H., et al. 2018. Oncol Lett. 16: 2009-2015. PMID: 30034553
  8. 自然杀伤 T 细胞上无甲状腺刺激素受体表达：免疫-内分泌相互作用的意义  |  Adamska-Fita, E., et al. 2024. Int J Mol Sci. 25: PMID: 39518994
  9. 促甲状腺激素（TSH）受体第三细胞质环不同区域的置换对组成型, 促甲状腺激素和促甲状腺激素受体自身抗体刺激的磷脂酰肌醇和 3',5'-环单磷酸腺苷信号的产生具有选择性影响。  |  Kosugi, S., et al. 1993. Mol Endocrinol. 7: 1009-20. PMID: 7901757
  10. 大鼠 TSHbeta 基因含有不同的反应元件，分别受维甲酸和甲状腺激素的调控。  |  Breen, JJ., et al. 1997. Mol Cell Endocrinol. 131: 137-46. PMID: 9296372
  11. 了解促甲状腺激素受体功能与结构的关系。  |  Sanders, J., et al. 1997. Baillieres Clin Endocrinol Metab. 11: 451-79. PMID: 9532334
  12. 功能性同二聚体糖蛋白激素：对激素作用和进化的影响。  |  Moyle, WR., et al. 1998. Chem Biol. 5: 241-54. PMID: 9646940

订购信息

产品名称产品编号规格价格数量收藏夹

TSHR 抗体 (3B12)

sc-53542
200 µg/ml
$316.00

TSHR 抗体 (3B12) AC

sc-53542 AC
500 µg/ml, 25% agarose
$416.00

TSHR 抗体 (3B12) HRP

sc-53542 HRP
200 µg/ml
$316.00

TSHR 抗体 (3B12) FITC

sc-53542 FITC
200 µg/ml
$330.00

TSHR 抗体 (3B12) PE

sc-53542 PE
200 µg/ml
$343.00

TSHR 抗体 (3B12) Alexa Fluor® 488

sc-53542 AF488
200 µg/ml
$357.00

TSHR 抗体 (3B12) Alexa Fluor® 546

sc-53542 AF546
200 µg/ml
$357.00

TSHR 抗体 (3B12) Alexa Fluor® 594

sc-53542 AF594
200 µg/ml
$357.00

TSHR 抗体 (3B12) Alexa Fluor® 647

sc-53542 AF647
200 µg/ml
$357.00

TSHR 抗体 (3B12) Alexa Fluor® 680

sc-53542 AF680
200 µg/ml
$357.00

TSHR 抗体 (3B12) Alexa Fluor® 790

sc-53542 AF790
200 µg/ml
$357.00

Which part of the receptor (TSHR) is targeted with this antibody?

Asked by: KwiatBerlin
Thank you for your question. This antibody was raised against amino acids 1-415 of TSHR of human origin. The antibody has not been epitope mapped. Please contact Technical Service if you have any further questions or need additional information.
Answered by: Tech Service
Date published: 2018-11-30

Can TSHR (3B12): sc-53542 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TSHR (3B12): sc-53542 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from no detection of TSHR in thyroid tissueThe IF staining of cryo sections of primary human tissue did not give any signal for the TSHR.
Date published: 2019-02-08
Rated 5 out of 5 by from strong bandThe antibody is very good ang useful, i got strong band
Date published: 2017-05-02
Rated 5 out of 5 by from Excellent!This antibody works great in immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.
Date published: 2017-01-20
Rated 5 out of 5 by from Successful experimentI use this product for my research, the WB（1/500） results is very strong with little or no background, the product quality is reliable.
Date published: 2016-12-18
Rated 5 out of 5 by from WB 1：1000体外表达重组蛋白，抗体好！2-5泳道分别是表达液上清，层析柱流穿，和两个洗脱峰，都是有阳性条带的。抗体真不错
Date published: 2016-12-05
Rated 4 out of 5 by from This antibody was used in a publication onThis antibody was used in a publication on rat FRTL-5 cells, PC-12 cell line, and rat thyroid cells - SCBT Publication Review
Date published: 2015-02-24
Rated 4 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by from IF and WB publishable data with human dermalIF and WB publishable data with human dermal microvascular endothelial cell line (HMEC-1) and thyroid tissue - SCBT Publication Review
Date published: 2013-05-13
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TSHR 抗体 (3B12) is rated 4.1 out of 5 by 8.
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