Date published: 2025-9-9

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transgelin 抗体 (6G6): sc-53932

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说明书
  • transgelin 抗体 6G6 是小鼠单克隆 IgG1 κ,transgelin抗体, 在31篇文献中引用,规格为200 µg/ml
  • 针对全长transgelin,human起源
  • transgelin 抗体 (6G6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 transgelin
  • 抗transgelin抗体(6G6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取transgelin (6G6): sc-53932免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 transgelin 抗体 (6G6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与transgelin 抗体 (6G6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

转髓鞘蛋白抗体(6G6)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人源的转髓鞘蛋白。抗转铁蛋白抗体 (6G6) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Transgelin 又称 SM22a,在调节平滑肌收缩和维持细胞骨架结构完整性方面起着至关重要的作用。Transgelin 主要位于平滑肌细胞中,有助于肌动蛋白丝的组织,并影响细胞的形状和运动。转胰蛋白存在于平滑肌细胞中对血管的正常功能和发育至关重要,有助于调节细胞的收缩状态。人类转铁蛋白基因(TAGLN)位于染色体 11q23.3,编码一种含有核因子结合基序的 201 氨基酸蛋白质,对平滑肌的转录调控非常重要。在胚胎发育过程中,转铁蛋白在各种类型的肌肉中都有表达,但成年后其表达仅限于血管和内脏平滑肌细胞,在心脏组织中的表达水平较低。转胰蛋白在几种转化细胞系中的表达下调,这表明转胰蛋白表达的减少可能是细胞转化的早期生物标志物。Transgelin 与肌动蛋白相互作用,在结合后不久促进肌动蛋白纤维凝胶化,其结合比例为 1:6 肌动蛋白单体,突出了 Transgelin 在细胞骨架动力学中的重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

transgelin 抗体 (6G6) 参考文献:

  1. 牛主动脉中一种新的 25 kDa 肌动蛋白结合蛋白的纯化, 表征和部分序列分析:SM22 同源物。  |  Kobayashi, R., et al. 1994. Biochem Biophys Res Commun. 198: 1275-80. PMID: 8117285
  2. Transgelin 的纯化和特性:一种对转化和形状变化敏感的肌动蛋白胶凝蛋白。  |  Shapland, C., et al. 1993. J Cell Biol. 121: 1065-73. PMID: 8501116
  3. 血清反应因子介导的调控网络管理平滑肌, 骨骼肌和心肌细胞中 SM22alpha 的转录。  |  Li, L., et al. 1997. Dev Biol. 187: 311-21. PMID: 9242426
  4. 成纤维细胞转球蛋白和平滑肌 SM22alpha 是同一种蛋白质,在许多细胞系中表达下调。  |  Lawson, D., et al. 1997. Cell Motil Cytoskeleton. 38: 250-7. PMID: 9384215
  5. 人类 SM22 基因(TAGLN)的表达和细胞遗传定位。  |  Camoretti-Mercado, B., et al. 1998. Genomics. 49: 452-7. PMID: 9615232

订购信息

产品名称产品编号规格价格数量收藏夹

transgelin 抗体 (6G6)

sc-53932
200 µg/ml
$316.00

transgelin (6G6): m-IgG Fc BP-HRP 套装

sc-528588
200 µg Ab; 10 µg BP
$354.00

transgelin (6G6): m-IgGκ BP-HRP 套装

sc-521000
200 µg Ab, 40 µg BP
$354.00

transgelin (6G6): m-IgG1 BP-HRP 套装

sc-543012
200 µg Ab; 20 µg BP
$354.00

transgelin 抗体 (6G6) AC

sc-53932 AC
500 µg/ml, 25% agarose
$416.00

transgelin 抗体 (6G6) HRP

sc-53932 HRP
200 µg/ml
$316.00

transgelin 抗体 (6G6) FITC

sc-53932 FITC
200 µg/ml
$330.00

transgelin 抗体 (6G6) PE

sc-53932 PE
200 µg/ml
$343.00

transgelin 抗体 (6G6) Alexa Fluor® 488

sc-53932 AF488
200 µg/ml
$357.00

transgelin 抗体 (6G6) Alexa Fluor® 546

sc-53932 AF546
200 µg/ml
$357.00

transgelin 抗体 (6G6) Alexa Fluor® 594

sc-53932 AF594
200 µg/ml
$357.00

transgelin 抗体 (6G6) Alexa Fluor® 647

sc-53932 AF647
200 µg/ml
$357.00

transgelin 抗体 (6G6) Alexa Fluor® 680

sc-53932 AF680
200 µg/ml
$357.00

transgelin 抗体 (6G6) Alexa Fluor® 790

sc-53932 AF790
200 µg/ml
$357.00

Are there any issues with using sc-53932: transgelin (6G6) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53932 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Good for WBI used this antibody for Western blot at 1:200 in 5% milk. It gave me a good band at around 22kDa and another one right above it.
Date published: 2017-10-09
Rated 5 out of 5 by from Specific and sharp bandthis antibody was tested on human aortic smooth muscle cells, and minimal background was observed, leaving a sharp and specific band with predicted size.
Date published: 2017-09-26
Rated 5 out of 5 by from Great functionWe applied for and received this antibody sample, for WB experiment, 1: 100 dilution of this antibody, the results are very good, the bands are very specific, very clear.
Date published: 2017-09-18
Rated 5 out of 5 by from Positive signal detected in WB in human transgelinPositive signal detected in WB in human transgelin transfected 293T and WI-38 whole cell lysates. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Strong band of interest observed in WB inStrong band of interest observed in WB in mouse transgelin transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Robust band in WB in URobust band in WB in U-2 OS, WI 38 and Hs68 whole cell lysates. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from ELISA publishable data with human seraELISA publishable data with human sera - SCBT Publication Review
Date published: 2014-02-18
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transgelin 抗体 (6G6) is rated 4.9 out of 5 by 7.
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