Date published: 2025-9-7
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TRAF1 Antibody (H-3) - Western Blotting - Image 52341
TRAF1 抗体 (H-3): sc-6253. 未转染: sc-117752 (A) 和小鼠 TRAF1 转染: sc-127696 (B) 293T 全细胞裂解液中 TRAF1 表达的 Western 印迹分析.
TRAF1 抗体 (H-3): sc-6253. 未转染: sc-117752 (A) 和小鼠 TRAF1 转染: sc-127696 (B) 293T 全细胞裂解液中 TRAF1 表达的 Western 印迹分析.

TRAF1 抗体 (H-3): sc-6253

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说明书
  • TRAF1 抗体 H-3 是小鼠单克隆 IgG1 κ，TRAF1抗体, 在31篇文献中引用，规格为200 µg/ml
  • 针对氨基酸173-295的抗性，映射到TRAF11的中心区域，human起源
  • TRAF1 抗体 (H-3) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 TRAF1
  • 抗TRAF1抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRAF1 (H-3): sc-6253的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 TRAF1 抗体 (H-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TRAF1 抗体 (H-3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TRAF 抗体 包括 TRAF1, TRAF2, TRAF3, TRAF4, TRAF5和TRAF6

TRAF1抗体（H-3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人源TRAF1蛋白。TRAF1单克隆抗体（H-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。TRAF1蛋白在介导肿瘤坏死因子（TNF）受体信号传导中起着至关重要的作用，特别是通过TNF受体1（TNF-R1）和TNF受体2（TNF-R2），它们是免疫反应和炎症的重要组成部分。TRAF1主要位于细胞质中，在那里TRAF1与TRAF2形成异源二聚体复合物，促进下游信号传导通路，从而引发细胞反应，如存活、增殖和分化。TRAF1与这些受体的相互作用对于调节免疫反应至关重要，TRAF1的失调与多种疾病有关，包括自身免疫性疾病和癌症。了解TRAF1的定位和功能对于阐明TRAF1在TNF受体介导的信号传导中的作用以及作为治疗靶点的潜力至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRAF1 抗体 (H-3) 参考文献:

  1. 两种 TNF 受体  |  Tartaglia, LA. and Goeddel, DV. 1992. Immunol Today. 13: 151-3. PMID: 1322675
  2. TRAF1对DMBA/太阳紫外线辐射诱导的皮肤癌发生至关重要。  |  Yamamoto, H., et al. 2017. J Invest Dermatol. 137: 1322-1332. PMID: 28131816
  3. TRAF1在人类健康和疾病中的信号传导。  |  Edilova, MI., et al. 2018. Front Immunol. 9: 2969. PMID: 30619326
  4. N6-甲基腺苷修饰的TRAF1通过METTL14依赖性方式调节肾细胞癌中的细胞凋亡和血管生成，从而促进舒尼替尼耐药性。  |  Chen, Y., et al. 2022. Mol Cancer. 21: 111. PMID: 35538475
  5. 依拉贝拉抑制TRAF1/NF-κB诱导的氧化DNA损伤，促进糖尿病足溃疡伤口愈合。  |  Hong, Y., et al. 2023. iScience. 26: 107601. PMID: 37664606
  6. 巨噬细胞中的TRAF1缺陷会加剧类风湿性关节炎的炎症。  |  Mirzaesmaeili, A. and Abdul-Sater, AA. 2024. Biomolecules. 14: PMID: 39062579
  7. 一种新型 RING 手指蛋白与 CD40 的细胞质结构域相互作用。  |  Hu, HM., et al. 1994. J Biol Chem. 269: 30069-72. PMID: 7527023
  8. CRAF1是TRAF的亲戚，它参与了CD40信号传导。  |  Cheng, G., et al. 1995. Science. 267: 1494-8. PMID: 7533327
  9. TNF 受体 1 相关蛋白 TRADD 发出细胞死亡和 NF-kappa B 激活信号。  |  Hsu, H., et al. 1995. Cell. 81: 495-504. PMID: 7758105
  10. Epstein-Barr 病毒转化蛋白 LMP1 与肿瘤坏死因子受体家族的信号蛋白结合。  |  Mosialos, G., et al. 1995. Cell. 80: 389-99. PMID: 7859281
  11. 与 75 kDa 肿瘤坏死因子受体胞质结构域缔合作用的新型假定信号转导子家族。  |  Rothe, M., et al. 1994. Cell. 78: 681-92. PMID: 8069916
  12. 细胞和病毒蛋白 TNF 受体超家族：激活, 成本刺激和死亡。  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429

订购信息

产品名称产品编号规格价格数量收藏夹

TRAF1 抗体 (H-3)

sc-6253
200 µg/ml
$316.00

TRAF1 (H-3): m-IgG Fc BP-HRP 套装

sc-526485
200 µg Ab; 10 µg BP
$354.00

TRAF1 (H-3): m-IgG1 BP-HRP 套装

sc-531858
200 µg Ab; 20 µg BP
$354.00

TRAF1 抗体 (H-3) AC

sc-6253 AC
500 µg/ml, 25% agarose
$416.00

TRAF1 抗体 (H-3) HRP

sc-6253 HRP
200 µg/ml
$316.00

TRAF1 抗体 (H-3) FITC

sc-6253 FITC
200 µg/ml
$330.00

TRAF1 抗体 (H-3) PE

sc-6253 PE
200 µg/ml
$343.00

TRAF1 抗体 (H-3) Alexa Fluor® 488

sc-6253 AF488
200 µg/ml
$357.00

TRAF1 抗体 (H-3) Alexa Fluor® 546

sc-6253 AF546
200 µg/ml
$357.00

TRAF1 抗体 (H-3) Alexa Fluor® 594

sc-6253 AF594
200 µg/ml
$357.00

TRAF1 抗体 (H-3) Alexa Fluor® 647

sc-6253 AF647
200 µg/ml
$357.00

TRAF1 抗体 (H-3) Alexa Fluor® 680

sc-6253 AF680
200 µg/ml
$357.00

TRAF1 抗体 (H-3) Alexa Fluor® 790

sc-6253 AF790
200 µg/ml
$357.00

Can TRAF1 (H-3): sc-6253 monoclonal antibody be used in WB and IP with primary culture of rat cortical neurons?

Asked by: Ricardo03
Thank you for your question. Anti-TRAF1 Antibody (H-3) is recommended for detection of TRAF1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. It is suitable for WB or IP work with cortical neuron cells from rat.
Answered by: Tech Support Europe
Date published: 2021-01-11

Can TRAF1 (H-3): sc-6253 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TRAF1 (H-3): sc-6253 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Western blottingThis antibody failed to detect the endogenous protein.
Date published: 2024-01-04
Rated 5 out of 5 by from Works good in WBThis Ab with 1:1000 dilution was used for the detection of human samples and the interested band could be observed clearly in WB image.
Date published: 2017-09-28
Rated 5 out of 5 by from Works very well in WBProduced beautiful WB results when I use 1:1000 in human sample
Date published: 2017-07-12
Rated 5 out of 5 by from Works well in FCMPE conjugated version sc-6253 PE performs well in Flow, and the unconjugated form does well in Western Blot and IF.
Date published: 2017-01-26
Rated 4 out of 5 by from WBright size but weak band could be observed in human samples by WB
Date published: 2017-01-17
Rated 5 out of 5 by from Able to publish data for the antibody usedAble to publish data for the antibody used in WB in human PBMCs.
Date published: 2015-06-25
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmicProduced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by from Publishable data in WB using the followingPublishable data in WB using the following human cells: 293T, Jurkat, A549 and BC-3. -SCBT Publication Review
Date published: 2015-05-17
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TRAF1 抗体 (H-3) is rated 4.5 out of 5 by 11.
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