TPX2抗体 (E-2) 推荐用于 human 来源的TPX2 WB, IP, IF, IHC(P) 和 ELISA检测

TPX2 抗体 (E-2): sc-271570

TPX2抗体 (E-2) is rated 4.3 out of 5 by 6.
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说明书

    • 抗-TPX2 抗体 E-2 是小鼠单克隆 IgG1 κ,TPX2抗体, 在1篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列448-747位于human物种的TPX2的C-terminus
    • TPX2 抗体 (E-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 TPX2
    • 抗-TPX2 抗体 (E-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取TPX2 (E-2): sc-271570免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) 是 TPX2 Antibody (E-2) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供TPX2 Antibody (E-2) (见下方订购信息).

Can TPX2 (E-2): sc-271570 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, TPX2 (E-2): sc-271570 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Good for IF This TPX2 Antibody (E-2), sc-271570, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Works in WB We received a sample and used it in human colon carcinoma cells. We detected a band at 120 kDa in western blot. (a bit higher than expected)
Date published: 2017-10-25
Rated 4 out of 5 by from GOOD The antibody is very good for WB to detect transacted TPX2-tagged protein. works great on immunoblotting at 1:750 in TBST
Date published: 2017-09-25
Rated 4 out of 5 by from western 条带较清晰,但试用装的量没有10ul westernblot条带较清晰,没有杂带。但是只有不到5ul的量,所以只敷了两三次,重复性还看不出
Date published: 2017-09-01
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in formalin-fixed HeLa cells. -SCBT QC
Date published: 2015-05-13
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