Date published: 2025-9-6

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Tns3 抗体 (C-2): sc-376367

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说明书
  • Tns3 抗体 C-2 是小鼠单克隆 IgG2a κ,Tns3抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列721-1020位于human物种的Tns3的内部区域内
  • Tns3 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Tns3
  • 抗Tns3抗体(C-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tns3 (C-2): sc-376367免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Tns3 Antibody (C-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Tns3 Antibody (C-2) 打包提供(请参阅下面的订购信息)。

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関連項目

Tns3抗体(C-2)是一种IgG2a κ小鼠单克隆Tns3抗体(也称为Tns3抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Tns3蛋白。Tns3抗体(C-2)既有非偶联的抗Tns3抗体形式,也有多种偶联形式的抗Tns3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Tensin(Tns)蛋白家族通过F-肌动蛋白结合和封端活动将肌动蛋白丝锚定在粘着斑上,从而参与细胞结构的维持。Tns蛋白还包含一个Src同源2(SH2)结构域,并具有磷酸化的能力,这表明它们在信号转导级联中起作用。这些不同的特性表明,Tns蛋白可能是细胞骨架和信号转导途径之间的重要联系。Tns3,也称为TEM6或TENS1,定位于质膜的粘着斑。它主要在甲状腺和胎盘中表达,但也可以在心脏、肝脏、大脑、前列腺、胰腺、肾脏、肺、骨骼肌和白细胞中找到。正如许多Tns3-/-小鼠的生长迟缓和死亡所暗示的,Tns3对于正常的生长和发育是必不可少的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tns3 抗体 (C-2) 参考文献:

  1. 表皮生长因子可调节一种新型田螺蛋白家族成员田螺蛋白3的酪氨酸磷酸化。  |  Cui, Y., et al. 2004. Mol Cancer Res. 2: 225-32. PMID: 15140944
  2. 小鼠 tensin3 失活会导致生长迟缓和出生后死亡。  |  Chiang, MK., et al. 2005. Dev Biol. 279: 368-77. PMID: 15733665
  3. Tensin3 是一种新型甲状腺特异性基因。  |  Maeda, I., et al. 2006. J Mol Endocrinol. 36: R1-8. PMID: 16461921
  4. Tensin:细胞骨架与信号转导之间的潜在联系。  |  Lo, SH., et al. 1994. Bioessays. 16: 817-23. PMID: 7840759
  5. 鸡心肌天丝蛋白高亲和性肌动蛋白封顶结构域的分子克隆, 表达和绘图。  |  Chuang, JZ., et al. 1995. J Cell Biol. 128: 1095-109. PMID: 7896874
  6. Tensin 与肌动蛋白的相互作用及其三个不同的肌动蛋白结合域的鉴定。  |  Lo, SH., et al. 1994. J Cell Biol. 125: 1067-75. PMID: 8195290
  7. 细胞在细胞外基质蛋白上的扩散会诱导 Tensin 的酪氨酸磷酸化。  |  Bockholt, SM. and Burridge, K. 1993. J Biol Chem. 268: 14565-7. PMID: 8325835
  8. tensin 和 auxilin 的 N 端结构域是磷酸酶同源物。  |  Haynie, DT. and Ponting, CP. 1996. Protein Sci. 5: 2643-6. PMID: 8976573

订购信息

产品名称产品编号规格价格数量收藏夹

Tns3 抗体 (C-2)

sc-376367
200 µg/ml
$316.00

Tns3 (C-2): m-IgG Fc BP-HRP 套装

sc-529859
200 µg Ab; 10 µg BP
$354.00

Tns3 (C-2): m-IgGκ BP-HRP 套装

sc-522929
200 µg Ab, 40 µg BP
$354.00

Tns3 (C-2): m-IgG2a BP-HRP 套装

sc-547392
200 µg Ab; 10 µg BP
$354.00

Tns3 抗体 (C-2) AC

sc-376367 AC
500 µg/ml, 25% agarose
$416.00

Tns3 抗体 (C-2) HRP

sc-376367 HRP
200 µg/ml
$316.00

Tns3 抗体 (C-2) FITC

sc-376367 FITC
200 µg/ml
$330.00

Tns3 抗体 (C-2) PE

sc-376367 PE
200 µg/ml
$343.00

Tns3 抗体 (C-2) Alexa Fluor® 488

sc-376367 AF488
200 µg/ml
$357.00

Tns3 抗体 (C-2) Alexa Fluor® 546

sc-376367 AF546
200 µg/ml
$357.00

Tns3 抗体 (C-2) Alexa Fluor® 594

sc-376367 AF594
200 µg/ml
$357.00

Tns3 抗体 (C-2) Alexa Fluor® 647

sc-376367 AF647
200 µg/ml
$357.00

Tns3 抗体 (C-2) Alexa Fluor® 680

sc-376367 AF680
200 µg/ml
$357.00

Tns3 抗体 (C-2) Alexa Fluor® 790

sc-376367 AF790
200 µg/ml
$357.00

Dear, I already ordered for this antibody, and I want to know the concentration or dilution for the immunostaining you recommend ? thank you in advance Hatem

Asked by: Hatem
Thank you for your question. The recommended starting dilution for immunostaining is 1:50 with a dilution range of 1:50-1:500. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page. If you have any further questions or concerns, please contact our Technical Service department by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-04-21

I am using Tns3 (C-2): sc-376367 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well for WB and IFThe antibody gives strong and clean signal in WB and was also working well in IF.
Date published: 2020-10-06
Rated 4 out of 5 by from Good results in IFI received a sample product a couple of months ago and tested it on MDA-MB-231 breast cancer cells, plated on collagen. This is the best Ab for TNS3 that I have used so far. It clearly labels adhesion site and this is strongly abolished upon transfection of an siRNA targeting TNS3. There is some cytosolic staining (which is reduced upon the KD) and some nuclear staining that seems unspecific, as it is still present in the KD cells (not visible in the images below, as they are taken with a confocal microscope focusing on the adhesion plane). Despite this, I think it is a very good antibody to assess TNS3 localisation in adhesive complexes.
Date published: 2018-02-01
Rated 5 out of 5 by from Good all aroundWell established clone for use in Western Blot, but also performs well in IF and IHC with a variety of fixation methods.
Date published: 2017-02-06
Rated 5 out of 5 by from Superb immunofluorescence focal adhesionsSuperb immunofluorescence focal adhesions and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-26
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Tns3 抗体 (C-2) is rated 4.8 out of 5 by 5.
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