Thimet oligopeptidase Antibody (4D6): sc-53565

Thimet oligopeptidase Antibody (4D6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Thimet oligopeptidase protein of mouse, rat, human, zebrafish, bovine, and porcine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Thimet oligopeptidase (4D6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thimet oligopeptidase, also known as soluble metallo-endopeptidase, is a cytoplasmic protein that plays a crucial role in the degradation of bioactive peptides, particularly neuropeptides less than 20 amino acids in length. Thimet oligopeptidase maintains peptide homeostasis and regulates neuropeptide signaling, which is vital for numerous physiological processes, including pain modulation and stress response. The gene encoding Thimet oligopeptidase is located on chromosome 19q13.3, and Thimet oligopeptidase is predominantly expressed in the testis, with detectable levels in the liver, lung, and kidney. Thimet oligopeptidase′s ability to degrade the β-Amyloid precursor protein and generate amyloidogenic fragments highlights its potential involvement in neurodegenerative diseases, making Thimet oligopeptidase a significant target for research in both basic and clinical settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Thimet oligopeptidase Antibody (4D6) References:

1. Expression of the thimet oligopeptidase gene is regulated by positively and negatively acting elements.  |  McCool, S. and Pierotti, AR. 2000. DNA Cell Biol. 19: 729-38. PMID: 11177571
2. Insulin-degrading enzyme rapidly removes the beta-amyloid precursor protein intracellular domain (AICD).  |  Edbauer, D., et al. 2002. J Biol Chem. 277: 13389-93. PMID: 11809755
3. Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase.  |  Oliveira, V., et al. 2002. Eur J Biochem. 269: 4326-34. PMID: 12199711
4. The cytosolic endopeptidase, thimet oligopeptidase, destroys antigenic peptides and limits the extent of MHC class I antigen presentation.  |  York, IA., et al. 2003. Immunity. 18: 429-40. PMID: 12648459
5. Crystal structure of human thimet oligopeptidase provides insight into substrate recognition, regulation, and localization.  |  Ray, K., et al. 2004. J Biol Chem. 279: 20480-9. PMID: 14998993
6. Pathway for degradation of peptides generated by proteasomes: a key role for thimet oligopeptidase and other metallopeptidases.  |  Saric, T., et al. 2004. J Biol Chem. 279: 46723-32. PMID: 15328361
7. Flexibility in substrate recognition by thimet oligopeptidase as revealed by denaturation studies.  |  Sigman, JA., et al. 2005. Biochem J. 388: 255-61. PMID: 15647004
8. Molecular cloning and primary structure of rat testes metalloendopeptidase EC 3.4.24.15.  |  Pierotti, A., et al. 1990. Biochemistry. 29: 10323-9. PMID: 2261476
9. Thimet oligopeptidase: similarity to 'soluble angiotensin II-binding protein' and some corrections to the published amino acid sequence of the rat testis enzyme.  |  McKie, N., et al. 1993. Biochem J. 295 (Pt 1): 57-60. PMID: 8216239

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thimet oligopeptidase Antibody (4D6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Thimet oligopeptidase Antibody (4D6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thimet oligopeptidase Antibody (4D6) Specifications

Alternate Product Name: Thimet oligopeptidase

Product Clone Name: 4D6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 56-72

Epitope Target: Thimet oligopeptidase

Epitope Species: human

Product Reactivity/Detection: Thimet oligopeptidase

Species Reactivity/Detection: mouse, rat, human, zebrafish, bovine, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Thimet oligopeptidase Antibody (4D6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin