Date published: 2025-9-6
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TET2 Antibody (C-7) - Western Blotting - Image 384019
TET2 抗体 (C-7) HRP: sc-398535 HRP. ES-D3 全细胞裂解液中 TET2 表达的直接 Western 印迹分析.
TET2 Antibody (C-7) - Western Blotting - Image 384019
TET2 抗体 (C-7) HRP: sc-398535 HRP. ES-D3 全细胞裂解液中 TET2 表达的直接 Western 印迹分析.
TET2 Antibody (C-7) - Western Blotting - Image 305739
TET2 抗体 (C-7): sc-398535. K-562 全细胞裂解液中 TET2 表达的 Western 印迹分析.
TET2 抗体 (C-7) HRP: sc-398535 HRP. ES-D3 全细胞裂解液中 TET2 表达的直接 Western 印迹分析.

TET2 抗体 (C-7): sc-398535

3.1(14)
寫評論提問

说明书
  • TET2 抗体 C-7 是小鼠单克隆 IgG2b κ，TET2抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的TET2的N-terminus
  • TET2 抗体 (C-7) 推荐用于 WB, IP, IF 和 ELISA，检测 和human 来源的 TET2
  • 抗TET2抗体（C-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TET2 (C-7): sc-398535的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是TET2 Antibody (C-7) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与TET2 Antibody (C-7) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外TET抗体，包括TET2

TET2抗体（C-7）是一种IgG2b κ小鼠单克隆TET2抗体，可通过WB、IP、IF和ELISA检测到人源的TET2蛋白。TET2抗体（C-7）既有无偶联的抗TET2抗体形式，也有多种偶联形式的抗TET2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。TET2（Tet癌基因家族成员2），也称为KIAA1546，是一种含有2002个氨基酸的蛋白质，在多种组织中表达，包括脑、肾、心、肺、肌肉和胃，并以三种选择性剪接异构体的形式存在。小鼠TET2也被称为Ayu17-449蛋白，被认为在肾脏的正常发育和整体肾功能以及全身激素分泌中发挥作用。编码人类TET2的基因位于4号染色体上，而编码小鼠TET2的基因位于3号染色体上。4号染色体编码了人类基因组的近6%，并拥有所有人类染色体中最大的基因沙漠（即没有蛋白质编码基因的基因组区域）。4号染色体上某些基因的缺陷与亨廷顿病、Ellis-van Creveld综合征、甲基丙二酸血症和多囊肾病有关。小鼠3号染色体上含有1300多个基因，其中一些表达酒精脱氢酶（ADHs）、钠通道修饰剂（SCNMs）、白细胞介素（ILs）和胰岛素受体相关（IRR）蛋白质。3号染色体上基因的缺陷与遗传性先天性面瘫（HCFP）、自发性结肠炎易感性增加、HIV-1相关性肾病、肾血管健康状况下降以及恶性散发性胰腺内分泌肿瘤有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TET2 抗体 (C-7) 参考文献:

  1. 预测人类不明基因的编码序列。XVIII.100 个新的大脑 cDNA 克隆的完整序列，它们在体外编码大型蛋白质。  |  Nagase, T., et al. 2000. DNA Res. 7: 273-81. PMID: 10997877
  2. 恶性散发性胰腺内分泌肿瘤中3号染色体的频繁缺失。  |  Guo, SS., et al. 2002. Mol Cell Endocrinol. 190: 109-14. PMID: 11997184
  3. TET1 是一种新型蛋白家族的成员，在含有 t(10;11)(q22;q23)的急性髓性白血病中与 MLL 融合。  |  Lorsbach, RB., et al. 2003. Leukemia. 17: 637-41. PMID: 12646957
  4. 预测小鼠 KIAA 基因同源物的编码序列：通过筛选从大小分馏文库中随机抽取的 cDNA 克隆的末端序列，确定了 500 个小鼠 KIAA 同源 cDNA 的完整核苷酸序列。  |  Okazaki, N., et al. 2003. DNA Res. 10: 167-80. PMID: 14621295
  5. 人类 2 号和 4 号染色体 DNA 序列的生成和注释。  |  Hillier, LW., et al. 2005. Nature. 434: 724-31. PMID: 15815621
  6. [通过基因诱捕法产生 Ayu17-449 基因敲除小鼠]。  |  Tang, H., et al. 2006. Yi Chuan. 28: 129-32. PMID: 16520305
  7. 小鼠新基因 Ayu17-449 的克隆和表达分析。  |  Tang, H., et al. 2006. Yi Chuan Xue Bao. 33: 413-9. PMID: 16722336
  8. 基因敲除小鼠模型中 Ayu17-449 基因表达及其导致的肾脏病理特征。  |  Tang, H., et al. 2008. Transgenic Res. 17: 599-608. PMID: 18288583

订购信息

产品名称产品编号规格价格数量收藏夹

TET2 抗体 (C-7)

sc-398535
200 µg/ml
$316.00

TET2 (C-7): m-IgG Fc BP-HRP 套装

sc-526315
200 µg Ab; 10 µg BP
$354.00

TET2 (C-7): m-IgG2b BP-HRP 套装

sc-549712
200 µg Ab; 10 µg BP
$354.00

TET2 抗体 (C-7) AC

sc-398535 AC
500 µg/ml, 25% agarose
$416.00

TET2 抗体 (C-7) HRP

sc-398535 HRP
200 µg/ml
$316.00

TET2 抗体 (C-7) FITC

sc-398535 FITC
200 µg/ml
$330.00

TET2 抗体 (C-7) PE

sc-398535 PE
200 µg/ml
$343.00

TET2 抗体 (C-7) Alexa Fluor® 488

sc-398535 AF488
200 µg/ml
$357.00

TET2 抗体 (C-7) Alexa Fluor® 546

sc-398535 AF546
200 µg/ml
$357.00

TET2 抗体 (C-7) Alexa Fluor® 594

sc-398535 AF594
200 µg/ml
$357.00

TET2 抗体 (C-7) Alexa Fluor® 647

sc-398535 AF647
200 µg/ml
$357.00

TET2 抗体 (C-7) Alexa Fluor® 680

sc-398535 AF680
200 µg/ml
$357.00

TET2 抗体 (C-7) Alexa Fluor® 790

sc-398535 AF790
200 µg/ml
$357.00

Hi I would like to receive a free testing sample of this antibody (TET2 Antibody (C-7): sc-398535), to try in IHC in mouse. It has very mixed reviews so I am not keen on purchasing until I am sure this ab works well in my hands. Thank you so much

Asked by: amartin3
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

What blocking solution should I use with TET2 (C-7): sc-398535 monoclonal antibody for Western blot?

Asked by: jenniferc
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 3 out of 5 by from good signal at expected MW1.1000 in RAW 264.7 cells: one nice band at expected MW, some other faint bands.
Date published: 2018-11-08
Rated 1 out of 5 by from Seems to be not really specificWe ordered a test sample and obtained several unspecific bands in WB whole HeLa lysates.
Date published: 2018-04-23
Rated 5 out of 5 by from Positive bandI used it to dilute the 1:300 with a specific band in the cell lysate, and it turned out to be very perfect.
Date published: 2017-10-27
Rated 2 out of 5 by from This antibody is not suitable for wb in SK cellsI used this antibody to WB (diluted 1:25), obtained several bands，the positive also have a strong nonspecific bands.
Date published: 2017-09-22
Rated 3 out of 5 by from GOODI used this antibody to WB (diluted 1400), obtained clear bands
Date published: 2017-09-15
Rated 4 out of 5 by from a good antibody for WBThis antibody generates a clean signal at the expected molecular weight (1:500 dilution, 40 ug of HeLa lysate). It stains the cytoplasm and may not work for IF in formaldehyde-fixed HeLa cells.
Date published: 2017-08-03
Rated 3 out of 5 by from have a band < 180 KDI have a try with the antibody(10 ug) which is free for try, and it has a band <180kd
Date published: 2017-07-10
Rated 2 out of 5 by from not suitable for WB and IFThis antibody is not suitable for wb in kidney tissue
Date published: 2017-07-08
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TET2 抗体 (C-7) is rated 3.1 out of 5 by 14.
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