Date published: 2025-9-6
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TEF-3 Antibody (N-G2) - Western Blotting - Image 368207
TEF-3 抗体 (N-G2): sc-101184. HeLa (A), Hep G2 (B), SW480 (C), MCF7 (D) 和 JEG-3 (E) 全细胞裂解液中 TEF-3 表达的 Western 印迹分析.
TEF-3 抗体 (N-G2): sc-101184. HeLa (A), Hep G2 (B), SW480 (C), MCF7 (D) 和 JEG-3 (E) 全细胞裂解液中 TEF-3 表达的 Western 印迹分析.

TEF-3 抗体 (N-G2): sc-101184

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寫評論提問

说明书
  • TEF-3 抗体 N-G2 是小鼠单克隆 IgG2a κ，TEF-3抗体, 在40篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组TEF-3
  • 推荐用于 mouse, rat 和 human 来源的TEF-3 WB, IP 和 ELISA检测
  • 目前，我们还没有完成TEF-3 抗体 (N-G2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Transcriptional Enhancer Factor 抗体 包括 TEF-1, TEF-3和TEF-4

TEF-3 抗体（N-G2）是一种 IgG2a κ 小鼠单克隆 TEF-3 抗体（也称为 TEAD4 抗体），可通过 WB、IP 和 ELISA 检测小鼠、大鼠和人源的 TEF-3 蛋白。TEF-3 抗体（N-G2）以非偶联的抗 TEF-3 抗体形式提供。TEF-3，也称为 TEAD4（TEA 结构域家族成员 4）、RTEF1、EFTR-2、TEFR-1、TCF13L1 或 hRTEF-1B，是转录增强因子（TEF）蛋白家族的 427 个氨基酸成员，其特点是存在一个 TEA DNA 结合域。TEF-3 定位于细胞核中，主要在骨骼肌中表达，通过特异性且非协同地结合在肌肉特异性基因启动子中发现的 M-CAT 基序，作为转录调节因子发挥作用，从而指导其随后的表达。TEF-3 包含一个 TEA DNA 结合域，并且由于选择性剪接事件而以多种异构体形式表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TEF-3 抗体 (N-G2) 参考文献:

  1. TEAD/TEF 转录因子利用 YAP65 的激活结构域，YAP65 是一种定位于细胞质的 Src/Yes 缔合作用蛋白。  |  Vassilev, A., et al. 2001. Genes Dev. 15: 1229-41. PMID: 11358867
  2. 转录增强因子-1 相关因子转基因小鼠出现心脏传导缺陷与缔合蛋白磷酸化改变有关。  |  Chen, HH., et al. 2004. Circulation. 110: 2980-7. PMID: 15520314
  3. 脂肪溶解酵母菌的 110kDa 谷胱甘肽转移酶由来自酿酒酵母的 TEF3 基因的同源物编码：重组蛋白的克隆、表达和同源物建模。  |  McGoldrick, S., et al. 2005. Biochem Biophys Res Commun. 337: 1125-32. PMID: 16229819
  4. 鉴定人眼血管内皮细胞和小鼠视网膜中 RTEF-1 的新型替代剪接异构体。  |  Appukuttan, B., et al. 2007. Invest Ophthalmol Vis Sci. 48: 3775-82. PMID: 17652751
  5. 转录因子 TEAD4 在哺乳动物发育初期指定了滋养层外胚层系。  |  Yagi, R., et al. 2007. Development. 134: 3827-36. PMID: 17913785
  6. 植入前小鼠胚胎中滋养层外胚层的形成需要 Tead4。  |  Nishioka, N., et al. 2008. Mech Dev. 125: 270-83. PMID: 18083014
  7. 转录增强因子 3 (TEF3) 在内皮细胞中介导唐氏综合征候选区 1 异构体 1 (DSCR1-1L) 的表达。  |  Liu, X., et al. 2008. J Biol Chem. 283: 34159-67. PMID: 18840614
  8. 人类 TEF3-1（转录因子 3 同工型 1）转录激活结构域的特征。  |  Qiao, C., et al. 2015. Arch Biochem Biophys. 569: 54-61. PMID: 25687649
  9. TEF3-1 的核定位促进了癌症的细胞周期进展和血管生成。  |  Teng, K., et al. 2016. Oncotarget. 7: 13827-41. PMID: 26885617
  10. 鉴定一种在静止成纤维细胞受到有丝分裂刺激后以及在成肌细胞分化过程中表达的小鼠 TEF-1 相关基因。  |  Hsu, DK., et al. 1996. J Biol Chem. 271: 13786-95. PMID: 8662936
  11. 含有 TEA/ATTS DNA 结合结构域的哺乳动物发育调控转录因子新家族。  |  Jacquemin, P., et al. 1996. J Biol Chem. 271: 21775-85. PMID: 8702974
  12. 克隆人类 RTEF-1（一种在骨骼肌中优先表达的转录增强因子-1 相关基因）：一个古老的多基因家族的证据。  |  Stewart, AF., et al. 1996. Genomics. 37: 68-76. PMID: 8921372

订购信息

产品名称产品编号规格价格数量收藏夹

TEF-3 抗体 (N-G2)

sc-101184
100 µg/ml
$333.00

what's the exact amino acid sequence this antibody targets? I am using this antibody for ChIP-seq and I wonder if it could also bind to other proteins. Hope to reply me soon. Thanks.

Asked by: Min1997
Anti-TEF-3 Antibody (N-G2): sc-101184 was raised against recombinant TEF-3 of human origin, but no epitope mapping has been performed for this antibody yet. Its exact epitope is not yet known. We offer a variety of different options for control experiments such as siRNA knockdown or CRISPR-based knockout products.
Answered by: Tech Support Europe
Date published: 2021-10-12

What is the total volume provided of TEF-3 Antibody (N-G2)?

Asked by: TinTin
Each vial of TEF-3 Antibody (N-G2): sc-101184 contains 100 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Service 11
Date published: 2017-01-09
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Rated 5 out of 5 by from Knockdown verifiedWorks well for western blot, and we've validated it using knockdown experiments.
Date published: 2022-12-02
Rated 5 out of 5 by from Very good antibodyWorks great for WB on mouse lysates, IF on mouse cell lines, I tried NIH 3T3 and HEK293, it works great!! I'm currently trying IP. let's see how it works?
Date published: 2020-09-22
Rated 5 out of 5 by from work well in wbi get the sample several month ago, it can detect the band in 293t
Date published: 2018-12-17
Rated 5 out of 5 by from Good for ChIPI bought this 3 weeks ago and am so happy that it works very well for ChIP with mouse pancreas tissue.
Date published: 2017-02-23
Rated 5 out of 5 by from good TEF-3 detectionGood antibody for endogenous and transfected TEF-3 detection. Works well in mouse and human samples.
Date published: 2017-01-13
Rated 3 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-06-27
Rated 3 out of 5 by from Good band seen in WB using HeLa whole cell lysateGood band seen in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-02
Rated 3 out of 5 by from Publishable data in WB using mouse embryoPublishable data in WB using mouse embryo -SCBT Publication Review
Date published: 2015-01-12
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TEF-3 抗体 (N-G2) is rated 4.1 out of 5 by 9.
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