Date published: 2025-9-9
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Tankyrase-1/2 Antibody (E-10) - Western Blotting - Image 375550
Tankyrase-1/2 抗体 (E-10): sc-365897. Jurkat (A), CCRF-CEM (B), RAW 264. 7 (C), PC-12 (D) 和 C6 (E) 全细胞裂解液中 Tankyrase-1/2 表达的 Western 印迹分析.
Tankyrase-1/2 抗体 (E-10): sc-365897. Jurkat (A), CCRF-CEM (B), RAW 264. 7 (C), PC-12 (D) 和 C6 (E) 全细胞裂解液中 Tankyrase-1/2 表达的 Western 印迹分析.

Tankyrase-1/2 抗体 (E-10): sc-365897

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说明书
  • Tankyrase-1/2 抗体 E-10 是小鼠单克隆 IgG2a κ，Tankyrase-1/2抗体, 在29篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Tankyrase-1的氨基酸745-1094
  • Tankyrase-1/2 抗体 (E-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Tankyrase-1 and Tankyrase-2
  • 抗Tankyrase-1/2抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tankyrase-1/2 (E-10): sc-365897的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Tankyrase-1/2 Antibody (E-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Tankyrase-1/2 Antibody (E-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Tankyrase抗体，包括Tankyrase-1和Tankyrase-2

Tankyrase-1/2抗体（E-10）是一种IgG2a κ小鼠单克隆Tankyrase-1/2抗体（也称为TNKS1/TNKS2抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Tankyrase-1/2蛋白。Tankyrase-1/2抗体（E-10）既可以是非结合的抗Tankyrase-1/2抗体形式，也可以是多种结合形式的抗Tankyrase-1/2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor结合物。Tankyrase-1（TRF 1-相互作用锚蛋白相关ADP-核糖聚合酶1）和密切相关的同源Tankyrase-2是多聚（ADP-核糖）聚合酶（PARPs），它们共定位并使用锚蛋白重复结构域结合不同的蛋白质，包括TRF-1（端粒重复结合因子1）、IRAP（胰岛素反应性氨肽酶）和TAB182。Tankyrase-1（也称为TNKS和TNKS1）和Tankyrase-2（也称为TNKS2、TNKL和TANK2）与同一组蛋白质相互作用，并可能在端粒和囊泡室中介导重叠功能。细胞核中Tankyrase-1的过表达促进端粒延长，表明Tankyrase 1可能调节端粒酶进入端粒复合物。Tankyrase-2在细胞核中的过表达从端粒中释放内源性TRF1，建立了Tankyrase-2自身作为PARP，TRF1作为ADP-核糖基化的受体，并表明Tankyrase-2在端粒中发挥作用的可能性。Tankyrase-2的锚蛋白（ANK）结构域包含五个为IRAP提供冗余结合位点的亚结构域。Tankyrase-2缺乏Tankyrase-1的N-末端组氨酸/脯氨酸/丝氨酸富集区，但含有相应的锚蛋白重复区、无菌α基序模块和聚（ADP-核糖）聚合酶同源结构域。编码Tankyrase-2的基因广泛表达，其mRNA转录物在骨骼肌和胎盘中特别丰富。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tankyrase-1/2 抗体 (E-10) 参考文献:

  1. 细胞周期依赖于端粒 PARP（tankyrase）在核孔复合体和中心体上的定域。  |  Smith, S. and de Lange, T. 1999. J Cell Sci. 112 (Pt 21): 3649-56. PMID: 10523501
  2. Tankyrase-2与tankyrase-1寡聚，并与TRF1（端粒重复结合因子1）和IRAP（胰岛素反应性氨基肽酶）结合。  |  Sbodio, JI., et al. 2002. Biochem J. 361: 451-9. PMID: 11802774
  3. 人类多聚（ADP-核糖）聚合酶的核定位信号是一种功能上与 DNA 结合和催化活性相分离的两部分元素。  |  Schreiber, V., et al. 1992. EMBO J. 11: 3263-9. PMID: 1505517
  4. Tankyrase 1和Tankyrase 2对小鼠胚胎发育至关重要，但却是多余的。  |  Chiang, YJ., et al. 2008. PLoS One. 3: e2639. PMID: 18612384
  5. 人类成纤维细胞多聚（ADP-核糖）聚合酶全长 cDNA 的核苷酸序列。  |  Uchida, K., et al. 1987. Biochem Biophys Res Commun. 148: 617-22. PMID: 3120710
  6. TDP-43是肌萎缩性脊髓侧索硬化症的核心蛋白，会被tankyrase-1和-2破坏稳定性。  |  McGurk, L., et al. 2020. J Cell Sci. 133: PMID: 32409565
  7. 作为坦克酶-1/2 抑制剂的异喹啉-1(2H)-酮衍生物的设计、合成和生物学评价。  |  Yao, H., et al. 2021. Pharmazie. 76: 132-137. PMID: 33849696
  8. 揭示 5-甲基-5-[4-(4-氧代-3H-喹唑啉-2-基)苯基]咪唑烷-2,4-二酮双靶向 Tankyrase 1 和 2 的结构作用机制：癌症治疗的新途径。  |  Peters, XQ., et al. 2022. Cell Biochem Biophys. 80: 505-518. PMID: 35637423
  9. 通过硅学和体外研究鉴定基于Honokiol的支架以设计Tankyrase 1/2抑制剂  |  Di Micco, S., et al. 2024. Chem Biodivers. e202401854. PMID: 39363757
  10. 一种人类端粒蛋白。  |  Chong, L., et al. 1995. Science. 270: 1663-7. PMID: 7502076
  11. 人类端粒蛋白 TRF1 对端粒长度的控制。  |  van Steensel, B. and de Lange, T. 1997. Nature. 385: 740-3. PMID: 9034193
  12. 人类端粒上的聚 ADP 核糖聚合酶 Tankyrase。  |  Smith, S., et al. 1998. Science. 282: 1484-7. PMID: 9822378

订购信息

产品名称产品编号规格价格数量收藏夹

Tankyrase-1/2 抗体 (E-10)

sc-365897
200 µg/ml
$316.00

Tankyrase-1/2 (E-10): m-IgGκ BP-HRP 套装

sc-522411
200 µg Ab, 40 µg BP
$354.00

Tankyrase-1/2 (E-10): m-IgG2a BP-HRP 套装

sc-546116
200 µg Ab; 10 µg BP
$354.00

Tankyrase-1/2 抗体 (E-10) AC

sc-365897 AC
500 µg/ml, 25% agarose
$416.00

Tankyrase-1/2 抗体 (E-10) HRP

sc-365897 HRP
200 µg/ml
$316.00

Tankyrase-1/2 抗体 (E-10) FITC

sc-365897 FITC
200 µg/ml
$330.00

Tankyrase-1/2 抗体 (E-10) PE

sc-365897 PE
200 µg/ml
$343.00

Tankyrase-1/2 抗体 (E-10) Alexa Fluor® 488

sc-365897 AF488
200 µg/ml
$357.00

Tankyrase-1/2 抗体 (E-10) Alexa Fluor® 546

sc-365897 AF546
200 µg/ml
$357.00

Tankyrase-1/2 抗体 (E-10) Alexa Fluor® 594

sc-365897 AF594
200 µg/ml
$357.00

Tankyrase-1/2 抗体 (E-10) Alexa Fluor® 647

sc-365897 AF647
200 µg/ml
$357.00

Tankyrase-1/2 抗体 (E-10) Alexa Fluor® 680

sc-365897 AF680
200 µg/ml
$357.00

Tankyrase-1/2 抗体 (E-10) Alexa Fluor® 790

sc-365897 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Tankyrase-1/2 (E-10): sc-365897?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-13

I would like to use Tankyrase-1/2 (E-10) for IF, what dilution should I use for the primary antibody?

Asked by: TinTin
Thank you for your question. We recommend a starting dilution of 1:50 for IF and IHC applications. Please contact Technical Service if you have any further questions or if you would like more information regarding the recommended protocol.
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 5 out of 5 by from 试用了一次，效果很好我上一周使用了Tankyrase-1/2 抗体 sc-365897，跑了abcam的tnks2抗体特异性不大，这次用了santa感觉结果还可以。
Date published: 2019-04-16
Rated 5 out of 5 by from Works for WB/IF/PLABought this antibody (Tank-1/2) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:2500 and IF/PLA 1:500.
Date published: 2017-08-15
Rated 5 out of 5 by from Great antibody for TNKS1/2This antibody is specific and is suitable for detection of TNKS2 in HEK293 cells. I used the antibody at 1:500, and the signal was pretty good
Date published: 2017-07-23
Rated 5 out of 5 by from an excellent antibodyvery good antibody for WB,recognises both TNKS1 and TNKS2
Date published: 2017-06-28
Rated 5 out of 5 by from Beautiful stainingBrilliant signal seen with methanol fixed cells in IF.
Date published: 2017-01-18
Rated 5 out of 5 by from Comparison between E-10 and H-340It is several times more sensitive than the discontinued rabbit polyclonal (H-340). It picks up TNKS as a single band in several extracts tested.
Date published: 2016-12-09
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-21
Rated 5 out of 5 by from Nice Western Blot data of TankyraseNice Western Blot data of Tankyrase-1/2 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-07-29
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Tankyrase-1/2 抗体 (E-10) is rated 5.0 out of 5 by 8.
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