Date published: 2025-9-6
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SYP/Synaptophysin Antibody (D-4) - Western Blotting - Image 385688
SYP 抗体 (D-4) HRP: sc-17750 HRP. IMR-32 (A), Neuro-2A (B), BE (2)-M17 (C) 和 SH-SY5Y (D) 全细胞裂解液以及大鼠脑 (E) 和小鼠脑 (F) 组织提取物中 SYP 表达的直接 Western 印迹分析.
SYP 抗体 (D-4) HRP: sc-17750 HRP. IMR-32 (A), Neuro-2A (B), BE (2)-M17 (C) 和 SH-SY5Y (D) 全细胞裂解液以及大鼠脑 (E) 和小鼠脑 (F) 组织提取物中 SYP 表达的直接 Western 印迹分析.

SYP/Synaptophysin 抗体 (D-4): sc-17750

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说明书
  • SYP/Synaptophysin 抗体 D-4 是小鼠单克隆 IgG1 κ，SYP/Synaptophysin抗体, 在177篇文献中引用，规格为200 µg/ml
  • 免疫human物种的synaptophysin (major synaptic vesicle protein 38)的氨基酸221-313
  • SYP/Synaptophysin 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SYP
  • 抗SYP/Synaptophysin抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SYP/Synaptophysin (D-4): sc-17750的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SYP/Synaptophysin 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SYP/Synaptophysin 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

SYP抗体（D-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类样本中的突触素蛋白（SYP）。抗SYP抗体（D-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物。突触素是一种主要存在于神经元和内分泌细胞中的整合膜蛋白，在突触囊泡的形成和回收中发挥着重要作用。突触素具有N-糖基化作用，并形成六聚体复合物，有助于形成大导通通道。突触素含有四个跨膜结构域，其功能类似于细胞间沟通的间隙连接。膜胆固醇与突触素发生特异性相互作用，影响囊泡的形成和动态变化。突触素（VAMP）与SYP形成复合物，在神经元发育过程中增加，但对外排作用并非必不可少。脑肌球蛋白V的尾部结构域与突触素II和SYP形成稳定的复合物，在去极化过程中钙离子涌入时复合物会解离，这表明突触功能中存在动态的蛋白质相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SYP/Synaptophysin 抗体 (D-4) 参考文献:

  1. 突触素-突触珠蛋白复合物：突触囊泡成熟的标志。  |  Becher, A., et al. 1999. J Neurosci. 19: 1922-31. PMID: 10066245
  2. 膜萌发和裂变中的脂质, 脂质修饰和脂质-蛋白质相互作用--从嗜内蛋白 A1 和突触素在突触囊泡内吞中的作用中获得启示。  |  Huttner, WB. and Schmidt, A. 2000. Curr Opin Neurobiol. 10: 543-51. PMID: 11084315
  3. 突触囊泡蛋白--突触素的内吞需要 COOH 端尾。  |  Linstedt, AD. and Kelly, RB. 1991. J Physiol (Paris). 85: 90-6. PMID: 1757893
  4. 鱼雷突触素：突触小泡蛋白的进化。  |  Cowan, D., et al. 1990. Brain Res. 509: 1-7. PMID: 2106365
  5. SYP-3343通过ROS介导的线粒体功能障碍对斑马鱼发育产生不利影响。  |  Chen, G., et al. 2022. J Hazard Mater. 437: 129382. PMID: 35749898
  6. Imp和Syp介导的神经干细胞在发育中的果蝇中枢神经系统中的时间模式。  |  Islam, IM. and Erclik, T. 2022. Genetics. 222: PMID: 35881070
  7. 环状Vps41可正向调节Syp，而Syp的过度表达可提高衰老小鼠的记忆能力。  |  Li, Y., et al. 2022. Front Mol Neurosci. 15: 1037912. PMID: 36533129
  8. SYP-3343通过调节内皮细胞行为驱动斑马鱼异常血管生成。  |  Chen, G., et al. 2023. Food Chem Toxicol. 174: 113671. PMID: 36796616
  9. Imp和Syp RNA结合蛋白通过调节转录因子在精确的神经元凋亡中的作用。  |  Guan, W., et al. 2024. Elife. 12: PMID: 39364747
  10. 囊泡相关膜蛋白和突触素在突触囊泡上有关联。  |  Calakos, N. and Scheller, RH. 1994. J Biol Chem. 269: 24534-7. PMID: 7929121
  11. 昆虫细胞中哺乳动物突触囊泡蛋白突触素的合成：囊泡生物发生模型。  |  Leimer, U., et al. 1996. Exp Cell Res. 224: 88-95. PMID: 8612696
  12. 脑肌球蛋白V是一种与突触囊泡缔合作用的运动蛋白：与突触素-突触蛋白复合体发生Ca2+依赖性相互作用的证据。  |  Prekeris, R. and Terrian, DM. 1997. J Cell Biol. 137: 1589-601. PMID: 9199173

订购信息

产品名称产品编号规格价格数量收藏夹

SYP/Synaptophysin 抗体 (D-4)

sc-17750
200 µg/ml
$316.00

SYP/Synaptophysin (D-4): m-IgG Fc BP-HRP 套装

sc-528294
200 µg Ab; 10 µg BP
$354.00

SYP/Synaptophysin (D-4): m-IgGκ BP-HRP 套装

sc-520635
200 µg Ab, 40 µg BP
$354.00

SYP/Synaptophysin (D-4): m-IgG1 BP-HRP 套装

sc-542855
200 µg Ab; 20 µg BP
$354.00

SYP/Synaptophysin 抗体 (D-4) AC

sc-17750 AC
500 µg/ml, 25% agarose
$416.00

SYP/Synaptophysin 抗体 (D-4) HRP

sc-17750 HRP
200 µg/ml
$316.00

SYP/Synaptophysin 抗体 (D-4) FITC

sc-17750 FITC
200 µg/ml
$330.00

SYP/Synaptophysin 抗体 (D-4) PE

sc-17750 PE
200 µg/ml
$343.00

SYP/Synaptophysin 抗体 (D-4) Alexa Fluor® 488

sc-17750 AF488
200 µg/ml
$357.00

SYP/Synaptophysin 抗体 (D-4) Alexa Fluor® 546

sc-17750 AF546
200 µg/ml
$357.00

SYP/Synaptophysin 抗体 (D-4) Alexa Fluor® 594

sc-17750 AF594
200 µg/ml
$357.00

SYP/Synaptophysin 抗体 (D-4) Alexa Fluor® 647

sc-17750 AF647
200 µg/ml
$357.00

SYP/Synaptophysin 抗体 (D-4) Alexa Fluor® 680

sc-17750 AF680
200 µg/ml
$357.00

SYP/Synaptophysin 抗体 (D-4) Alexa Fluor® 790

sc-17750 AF790
200 µg/ml
$357.00

We used the SYP(D-4) with the mouse IgG binding protein (488) on fixed mouse brains but did not see any signal. What can you recommend? Do we need a specific antigen retrieval step for this antibody or should we try traditional secondary antibodies?

Asked by: racsumbr
Thank you for your question. You can find the recommended IHC protocol on this link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com, and we would be happy to help you troubleshoot the antibody!
Answered by: Tech Service
Date published: 2017-09-21

Can you recommend a positive control to use with SYP (D-4): sc-17750 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend IMR-32 (sc-2409) or MDA-MB-231 (sc-2232) whole cell lysate, rat brain extract (sc-2392) or mouse brain extract (sc-2253). whole cell lysates to use as positive controls as we have Western blot data showing specific detection of SYP in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-11

What secondary is best to use with SYP (D-4): sc-17750 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with SYP (D-4): sc-17750, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 5 out of 5 by from Clear signal for Western blot analysisThis antibody works nicely in Western blot. It gives a strong and specific signal corresponding to the expected molecular weight in both murine and human differentiated neuroblastoma cell lines. Antibody dilution 1:1.000, overnight incubation at 4°C in TBST + 5% no-fat dry milk.
Date published: 2021-12-12
Rated 5 out of 5 by from 蛍光抗体法に使える1:200で希釈して1ug/mlにしてマウスのneuroblastomaを染色すると細胞内の小胞が見えました。
Date published: 2021-06-15
Rated 3 out of 5 by from bisogna utilizzarlo molto concentratoSC-17750-SAMPLE Anticorpo SYP (D-4) L'anticorpo rivela la banda desiderata (western blot) solo se utilizzato alla diluzione di 1:200 in BSA 1%. In altri modi non funziona in cellule che si differenziano verso il fenotipo neuroendocrino, non cellule neuronali. Il secondario, invece, era utilizzato alla concentrazione di 1:2000 in milk al 3%
Date published: 2020-11-09
Rated 5 out of 5 by from Ottimo funzionamento in WBHo testato l'anticorpo in WB contro lisati di neuroni ippocampali in coltura e ottenuto ottimi risultati.
Date published: 2020-02-25
Rated 5 out of 5 by from Western blot of synaptophysinGood Western blot data of synaptophysin (SYP) expression in rat hippocampal brain tissue. Specific band at 1:200 dilution.
Date published: 2019-08-13
Rated 5 out of 5 by from Remarkable effectThis is a very good antibody than I bought from other companies. I used to tried many different samples, but its a wast of time. thanks to santa, thanks for your good products. You should try your best to provide more antibodies of high quality like this to us researchers.
Date published: 2019-02-20
Rated 5 out of 5 by from ReccommendedWorks great on neuronal cell line samples in WB and ICC. Staining is highly specific in both applications.
Date published: 2018-05-23
Rated 5 out of 5 by from Excellent antibodyIt is highly sensitive at 1-1000 dilution and shows no non-specific bands.
Date published: 2018-05-02
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SYP/Synaptophysin 抗体 (D-4) is rated 4.7 out of 5 by 29.
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