SV40 T Ag Antibody (PAb419): sc-58665

SV40 T Ag Antibody (PAb419) is a mouse monoclonal IgG2a antibody that detects SV40 T antigen of SV40 species reactivity by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SV40 T antigen antibody (PAb419) plays a crucial role in cancer research, as SV40 T antigen is a key player in viral transformation of host cells. SV40 T antigen is primarily located in the nucleus, where SV40 T antigen exerts effects by interacting with critical cellular proteins such as tumor suppressor p53 and retinoblastoma protein (Rb). Nuclear localization of SV40 T antigen is essential for function, as this positioning allows disruption of normal cell cycle regulation and promotion of uncontrolled cell proliferation. Through binding to p53, SV40 T antigen inhibits tumor-suppressive functions, while interaction with Rb prevents Rb-E2F complex from regulating transcription of genes necessary for cell cycle progression. Understanding mechanisms by which SV40 T antigen manipulates cellular pathways is vital for developing therapeutic strategies against cancers associated with viral infections.

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SV40 T Ag Antibody (PAb419) References:

1. Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated ras oncogene and SV40 T-antigen.  |  Su, LN. and Little, JB. 1992. Int J Radiat Biol. 62: 201-10. PMID: 1355514
2. Involvement of chromatin and histone deacetylation in SV40 T antigen transcription regulation.  |  Valls, E., et al. 2007. Nucleic Acids Res. 35: 1958-68. PMID: 17341466
3. Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line.  |  Song, XG., et al. 2013. World J Gastroenterol. 19: 8020-7. PMID: 24307795
4. Immortalization of Human Fetal Hepatocyte by Ectopic Expression of Human Telomerase Reverse Transcriptase, Human Papilloma Virus (E7) and Simian Virus 40 Large T (SV40 T) Antigen Towards Bioartificial Liver Support.  |  Giri, S. and Bader, A. 2014. J Clin Exp Hepatol. 4: 191-201. PMID: 25755560
5. Simian virus 40 (SV40)-transgenic mice that develop tumors are specifically tolerant to SV40 T antigen.  |  Faas, SJ., et al. 1987. J Exp Med. 165: 417-27. PMID: 3029269
6. Establishment of five immortalized human ovarian surface epithelial cell lines via SV40 T antigen or HPV E6/E7 expression.  |  Shin, HY., et al. 2018. PLoS One. 13: e0205297. PMID: 30296284
7. Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors.  |  Brinster, RL., et al. 1984. Cell. 37: 367-79. PMID: 6327063
8. Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.  |  Joo, WS., et al. 1997. J Virol. 71: 3972-85. PMID: 9094674
9. Topoisomerase I stimulates SV40 T antigen-mediated DNA replication and inhibits T antigen's ability to unwind DNA at nonorigin sites.  |  Simmons, DT., et al. 1998. Virology. 242: 435-43. PMID: 9514975
10. Characterization of the nucleotide binding properties of SV40 T antigen using fluorescent 3'(2')-O-(2,4,6-trinitrophenyl)adenine nucleotide analogues.  |  Huang, SG., et al. 1998. Biochemistry. 37: 15336-44. PMID: 9799494

The preferred SV40 T Ag antibody is SV40 T Ag Antibody (Pab 101): sc-147.