Date published: 2025-9-7

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serum reponse factor /SRF 抗体 (A-11): sc-25290

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说明书
  • serum reponse factor /SRF 抗体 A-11 是小鼠单克隆 IgM κ,serum reponse factor /SRF抗体, 在23篇文献中引用,规格为200 µg/ml
  • 免疫human物种的SRF的氨基酸209-508
  • 推荐用于 mouse, rat 和 human 来源的SRF WB, IP, IF 和 ELISA检测
  • 另提供琼脂糖偶联抗体,规格为琼脂糖装,可用于IP实验;HRP 偶联抗体用于WB, IHC(P)和ELISA实验;以及藻红蛋白、FITC、Alexa Fluor® 488Alexa Fluor® 647 偶联抗体,规格为用于IF、IHC(P)和FCM实验
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-25290 X, 200 µg/0.1 ml)
  • 关于如何获取serum reponse factor /SRF (A-11): sc-25290免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成serum reponse factor /SRF 抗体 (A-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

SRF抗体(A-11)是一种小鼠单克隆IgM kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类样本中的血清反应因子(SRF)。SRF抗体(A-11)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。SRF (A-11) 抗体靶向 SRF,一种与血清反应元件 (SRE) 结合的转录因子,可调节细胞对生长刺激的反应。SRF 与辅助蛋白 p62TCF 在 c-Fos SRE 处形成三元复合物,以有效传递信号,调节基因表达以响应外部信号。SRF还与相关的Ets结构域蛋白相互作用,包括Elk-1和SRF辅助蛋白-1(SAP-1),它们具有相似的DNA结合特性,有助于形成三元复合物。翻译后修饰,包括SRF内保守位点的磷酸化,对于各种细胞环境中的功能调节和活性至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

serum reponse factor /SRF 抗体 (A-11) 参考文献:

  1. SAP-1的特征,SAP-1是一种被血清反应因子招募到c-fos血清反应元件上的蛋白质。  |  Dalton, S. and Treisman, R. 1992. Cell. 68: 597-612. PMID: 1339307
  2. SRF 及其附属蛋白三元复合物的空间灵活性。  |  Treisman, R., et al. 1992. EMBO J. 11: 4631-40. PMID: 1425594
  3. elk-1 结构域负责自主 DNA 结合, SRE:SRF 相互作用以及 DNA 结合的负调控。  |  Rao, VN. and Reddy, ES. 1992. Oncogene. 7: 2335-40. PMID: 1437156
  4. 人类 T 细胞白血病病毒 1 型 Tax 可增强血清反应因子 DNA 的结合并改变位点选择。  |  Winter, HY. and Marriott, SJ. 2007. J Virol. 81: 6089-98. PMID: 17376895
  5. 表皮生长因子和其他有丝分裂原诱导人星形细胞瘤和其他细胞中的蛋白复合物与 c-fos 血清反应元件结合。  |  Malik, RK., et al. 1991. J Biol Chem. 266: 8576-82. PMID: 1902475
  6. SRE:一种生长因子反应性转录调节器。  |  Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
  7. 肌动蛋白 CArG 结合因子与 c-fos 血清反应因子没有区别。  |  Boxer, LM., et al. 1989. Mol Cell Biol. 9: 515-22. PMID: 2710114
  8. 编码 SRF(一种与 c-fos 血清反应元件结合的转录因子)的 cDNA 克隆的分离和特性。  |  Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
  9. 生长因子响应转录因子复合物的功能分析。  |  Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450

订购信息

产品名称产品编号规格价格数量收藏夹

serum reponse factor /SRF 抗体 (A-11)

sc-25290
200 µg/ml
$316.00

serum reponse factor /SRF 抗体 (A-11) AC

sc-25290 AC
500 µg/ml, 25% agarose
$416.00

serum reponse factor /SRF 抗体 (A-11) Alexa Fluor® 488

sc-25290 AF488
200 µg/ml
$357.00

serum reponse factor /SRF 抗体 (A-11) Alexa Fluor® 647

sc-25290 AF647
200 µg/ml
$357.00

serum reponse factor /SRF 抗体 (A-11) FITC

sc-25290 FITC
200 µg/ml
$330.00

serum reponse factor /SRF 抗体 (A-11) HRP

sc-25290 HRP
200 µg/ml
$316.00

serum reponse factor /SRF 抗体 (A-11) PE

sc-25290 PE
200 µg/ml
$343.00

serum reponse factor /SRF 抗体 (A-11) X

sc-25290 X
200 µg/0.1 ml
$316.00

When should the TransCruz reagent sc-25290 X be used instead of the standard form sc-25290?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ottimo funzionamentoHo eseguito western blott di proteine nucleari di cuore murino ed ho ottenuto una banda molto nitida
Date published: 2021-12-13
Rated 1 out of 5 by from poor resultsAt first time we tried 1:200 dilution and we could detect some week band at the the position corresponding to the molecular weight of SRF. However, after that we could never detect the band at all even at 1:100 dilution.
Date published: 2018-02-12
Rated 4 out of 5 by from GOOD for mES WBI could detect the SRF band with mES cell line lysate, but there are also some non-specific band.
Date published: 2017-09-27
Rated 1 out of 5 by from Not working well in Gastric cancer cell linesVery weak and many non specific WB signals in gastric cancer cell lines
Date published: 2017-06-21
Rated 5 out of 5 by from specific bandSRF antibody shows high express in rat spleen tissue. We use dilution 1:500 got specific band,strong wb with low background,
Date published: 2017-02-25
Rated 5 out of 5 by from A constitutive promoter elementOne of the features that can be used is that the serum response factor is a transcription factor that mediates the transient response of many cell gene stimuli, and we arrange it as a constitutive promoter element in our experiments.
Date published: 2017-02-12
Rated 5 out of 5 by from detect SRF by WBwe use this antibody to detect the target protein SRF and band is very strong and no background.
Date published: 2017-02-11
Rated 5 out of 5 by from Results were great.SRF antibody gives very good signal, good specificity, Very efficient antibody indeed.
Date published: 2017-02-07
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serum reponse factor /SRF 抗体 (A-11) is rated 4.3 out of 5 by 17.
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