Date published: 2025-9-7
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SQSTM1/p62 Antibody (formerly ZIP) - Western Blotting - Image 12723
SQSTM1 抗体 (A-6): sc-48402. SK-LMS-1 (A), MDA-MB-231 (B), SK-BR-3 (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 SQSTM1 表达的 Western 印迹分析.
SQSTM1 抗体 (A-6): sc-48402. SK-LMS-1 (A), MDA-MB-231 (B), SK-BR-3 (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 SQSTM1 表达的 Western 印迹分析.

SQSTM1/p62 抗体 (A-6): sc-48402

4.5(19)
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说明书
  • SQSTM1/p62抗体(A-6)是小鼠单克隆IgG1 κ, 在89篇文献中引用，规格为200 µg/ml
  • 免疫human物种的SQSTM1的氨基酸151-440
  • 抗-SQSTM1/p62 抗体 (A-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SQSTM1
  • 抗SQSTM1/p62抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SQSTM1/p62 (A-6): sc-48402的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 SQSTM1/p62 抗体 (A-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SQSTM1/p62 抗体 (A-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 SQSTM1 抗体包括 SQSTM1-L1

SQSTM1 抗体 (A-6) 是一种小鼠单克隆 IgG1 kappa kappa 轻链抗体，可特异性检测包括小鼠、大鼠和人在内的多个物种的 SQSTM1（又称 p60、p62、A170、DMRV、OSIL、PDB3、ZIP3、p62B、EBIAP、NADGP 和 FTDALS3）。抗 SQSTM1 抗体 (A-6) 靶向人 SQSTM1 蛋白的 151-440 氨基酸区，可用于免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP) 和酶联免疫吸附试验 (ELISA)。SQSTM1 是一种多功能适配蛋白，参与多种细胞过程，包括自噬，SQSTM1 通过与自噬体上的 LC3 相互作用，促进泛素化蛋白质的选择性降解。此外，SQSTM1 还与 TRAF6 和 Keap1 等关键信号蛋白相互作用，前者在 NF-κB 信号通路中发挥关键作用，后者则调节参与氧化应激反应的 Nrf2 通路。这些相互作用对于维持细胞平衡、调节炎症、细胞凋亡和氧化应激反应至关重要。SQSTM1 与泛素化蛋白的结合还受到翻译后修饰（包括磷酸化和泛素化）的进一步调节，这些修饰增强了结合亲和力以及信号通路和自噬通量中的功能多样性。SQSTM1 (A-6) 抗体既有非共轭形式，也有共轭形式，如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物，为研究人员提供了满足不同实验需求的多种选择。SQSTM1 的泛素相关（UBA）结构域发生突变，会破坏聚合体螯合和细胞存活的正常功能，导致骨重塑异常和骨脆性增加。SQSTM1 单克隆抗体 (A-6) 使研究人员能够全面了解 SQSTM1 的分子相互作用和调控机制，从而促进对疾病机理的理解和靶向治疗策略的开发。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SQSTM1/p62 抗体 (A-6)

sc-48402
200 µg/ml
$316.00

SQSTM1/p62 (A-6): m-IgG Fc BP-HRP 套装

sc-526748
200 µg Ab; 10 µg BP
$354.00

SQSTM1/p62 (A-6): m-IgG1 BP-HRP 套装

sc-532121
200 µg Ab; 20 µg BP
$354.00

SQSTM1/p62 抗体 (A-6) AC

sc-48402 AC
500 µg/ml, 25% agarose
$416.00

SQSTM1/p62 抗体 (A-6) HRP

sc-48402 HRP
200 µg/ml
$316.00

SQSTM1/p62 抗体 (A-6) FITC

sc-48402 FITC
200 µg/ml
$330.00

SQSTM1/p62 抗体 (A-6) PE

sc-48402 PE
200 µg/ml
$343.00

SQSTM1/p62 抗体 (A-6) Alexa Fluor® 488

sc-48402 AF488
200 µg/ml
$357.00

SQSTM1/p62 抗体 (A-6) Alexa Fluor® 546

sc-48402 AF546
200 µg/ml
$357.00

SQSTM1/p62 抗体 (A-6) Alexa Fluor® 594

sc-48402 AF594
200 µg/ml
$357.00

SQSTM1/p62 抗体 (A-6) Alexa Fluor® 647

sc-48402 AF647
200 µg/ml
$357.00

SQSTM1/p62 抗体 (A-6) Alexa Fluor® 680

sc-48402 AF680
200 µg/ml
$357.00

SQSTM1/p62 抗体 (A-6) Alexa Fluor® 790

sc-48402 AF790
200 µg/ml
$357.00

Boa tarde, O anti-corpo em questão está validado para amostras de cérebro de ratinho? Para WB e IF? Obrigada Helena

Asked by: 1D4B
Thank you for your question. SQSTM1/p62 Antibody (A-6): sc-48402 is recommended for detection of SQSTM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It would be suitable for your planned experiment.
Answered by: Tech Support Europe
Date published: 2025-03-21

I am using SQSTM1 (A-6): sc-48402 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2023-06-20
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Rated 5 out of 5 by from ottimo funzionamentosono molto soddisfatta per l'acquisto di questo anticorpo sc-48402
Date published: 2021-10-04
Rated 4 out of 5 by from Good signal in western blottingDilution 1:1000 Western blotting in cell lysate of different type of cells Godd signal at the expected molecular weight
Date published: 2019-12-11
Rated 1 out of 5 by from cannot detect the bandI used this antibody to detect protein but I cannot see 62kDa band. It was so strange even i changed to other cell lines. there are a numerous non- specific band and I got a very strong signal at appox. 99kDa position. I really cannot understand this situation.
Date published: 2018-07-20
Rated 3 out of 5 by from non specific bands in WBI used this antibody in WB experiments in human fibroblasts. I got a lot of non specific bands in other places.
Date published: 2018-06-06
Rated 5 out of 5 by from Works wellI recently received this antibody in the Hinck Lab, and I am using it for WB. The antibody shows great detection in WB analyses, and no background.
Date published: 2018-05-09
Rated 5 out of 5 by from Nice bandWe checked the antibody in MDCK cells (canine). The conditions were: - 50 u g protein per lane - 1:1,000 dilution in BSA (1% in TBST) - incubation overnight at 4ºC with agitation - secondary antibody: 1:10,000 dilution in BSA (1% TBST)
Date published: 2018-04-09
Rated 3 out of 5 by from Ok signal in mousce tissueOK signal in mouse muscle tissue. No signal in mouse muscle cells.
Date published: 2018-02-22
Rated 5 out of 5 by from High specificity with dilutions around 1:200This antibody works well in Western blot (1:200) for mouse brain in alcoholism model
Date published: 2017-12-25
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SQSTM1/p62 抗体 (A-6) is rated 4.5 out of 5 by 19.
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