spectrin beta III抗体 (A-8) 推荐用于 mouse, rat 和 human 来源的spectrin β III WB, IP, IF 和 ELISA检测

spectrin β III 抗体 (A-8): sc-515737

spectrin β III抗体 (A-8) is rated 2.0 out of 5 by 1.
  • y_2021, m_6, d_18, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_zh_CN, sid_515737, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
loading
5
0
4
0
3
0
2
1
1
0

说明书

    • 抗-spectrin beta III 抗体 A-8 是小鼠单克隆 IgG1 (kappa轻链),spectrin β III抗体,规格为200 µg/ml
    • 特异性抗原位于human物种的spectrin β III的N-terminus的氨基酸2-22之间
    • 抗-spectrin beta III 抗体 (A-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 spectrin β III
    • 抗-spectrin beta III 抗体 (A-8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取spectrin β III (A-8): sc-515737免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 spectrin β III Antibody (A-8) for WB 的首选应用二抗。这些试剂现在以套装形式提供spectrin β III Antibody (A-8)。 (见下方订购信息).
loading

How much sample do you recommend loading on the gel for Western blot detection using spectrin β III (A-8): sc-515737?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2021, m_6, d_18, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_515737, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 2 out of 5 by from it didn't work on my IHC human tissue I tried this antibody on my human spectrin B III positive control slides IHC, and repeat it, both time didn't work.
Date published: 2017-01-20
  • y_2021, m_6, d_18, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_zh_CN, sid_515737, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT