Spastin Antibody (A-2): sc-271247

Spastin Antibody (A-2) is a mouse monoclonal IgG2a light chain antibody that detects Spastin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Spastin is a member of the AAA protein family, characterized by its ATPase domain, and plays a crucial role in various cellular processes, including intracellular motility, membrane trafficking, and organelle biogenesis. This 616 amino acid protein is predominantly localized in the nucleus and cytoplasm, where Spastin is essential for microtubule dynamics, influencing the stability and organization of the cytoskeleton. Proper functioning of Spastin is vital, as mutations in the Spastin gene (SPAST, SPG4) are linked to the most common form of hereditary spastic paraplegia, specifically spastic paraplegia 4. This autosomal dominant disorder is characterized by progressive weakness and stiffness in the lower extremities due to retrograde axonopathy affecting corticospinal motor neurons. Notably, SPAST-specific mutations account for approximately 40% of all autosomal dominant hereditary spastic paraplegias, underscoring Spastin′s importance in maintaining neuronal health and function. Anti-Spastin antibody (A-2) serves as an invaluable tool for researchers studying the molecular mechanisms underlying these neurological disorders and the broader implications of Spastin in cellular dynamics.

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Spastin Antibody (A-2) References:

1. Spastin binds to lipid droplets and affects lipid metabolism.  |  Papadopoulos, C., et al. 2015. PLoS Genet. 11: e1005149. PMID: 25875445
2. The AAA protein spastin possesses two levels of basal ATPase activity.  |  Fan, X., et al. 2018. FEBS Lett. 592: 1625-1633. PMID: 29710391
3. Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules.  |  Kuo, YW., et al. 2019. Proc Natl Acad Sci U S A. 116: 5533-5541. PMID: 30837315
4. Spastin MIT Domain Disease-Associated Mutations Disrupt Lysosomal Function.  |  Allison, R., et al. 2019. Front Neurosci. 13: 1179. PMID: 31787869
5. Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching.  |  Ji, ZS., et al. 2021. Neural Regen Res. 16: 2549-2556. PMID: 33907047
6. Phosphorylation of Spastin Promotes the Surface Delivery and Synaptic Function of AMPA Receptors.  |  Chen, L., et al. 2022. Front Cell Neurosci. 16: 809934. PMID: 35418834
7. Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia.  |  Rizo, T., et al. 2022. Brain. 145: 3131-3146. PMID: 36103408
8. Spastin Promotes the Migration and Invasion Capability of T98G Glioblastoma Cells by Interacting with Pin1 through Its Microtubule-Binding Domain.  |  Temizci, B., et al. 2023. Cells. 12: PMID: 36766769
9. 14-3-3 protein augments the protein stability of phosphorylated spastin and promotes the recovery of spinal cord injury through its agonist intervention.  |  Liu, Q., et al. 2024. Elife. 12: PMID: 38231910
10. Spastin and alsin protein interactome analyses begin to reveal key canonical pathways and suggest novel druggable targets.  |  Helmold, BR., et al. 2025. Neural Regen Res. 20: 725-739. PMID: 38886938

The preferred Spastin antibody is Spastin Antibody (Sp 3G11/1): sc-53443.