Sp4抗体 (B-1) 推荐用于 mouse, rat 和 human 来源的Sp4 WB, IP, IF, IHC(P) 和 ELISA检测

Sp4 抗体 (B-1): sc-390124

Sp4抗体 (B-1) is rated 5.0 out of 5 by 3.
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说明书

    • 抗-Sp4 抗体 B-1 是小鼠单克隆 IgG1 κ,Sp4抗体, 在6篇文献中引用,规格为200 µg/ml
    • 免疫human物种的Sp4的氨基酸171-440
    • 抗-Sp4 抗体 (B-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Sp4
    • 抗-Sp4 抗体 (B-1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于ChIP的TransCruz试剂(sc-390124 X, 200 µg/0.1 ml)
    • 关于如何获取Sp4 (B-1): sc-390124免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 Sp4 Antibody (B-1) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Sp4 Antibody (B-1) (见下方订购信息).
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I also use this antibody and got clear bands in WB; however, I didn't detect fluorescence signal in IFA. If possible, could you tell me the detail protocol to perform immunofluorescence staining of formalin-fixed A-431 cells as described above?

Asked by: NH001
Thank you for your question. Here is a link to the recommended protocol for IF. For this antibody we recommend a starting dilution of 1:50. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining Please contact Technical Service or your local distributor for any further assistance with this antibody.
Answered by: Tech Service
Date published: 2019-10-08

I am using Sp4 (B-1): sc-390124 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Clear bands! I got clear bands,using 20uL of the antibody in 7mL 3% BSA solution
Date published: 2019-02-04
Rated 5 out of 5 by from Good Specific bands and no background. Used for pancreatic cancer cells
Date published: 2017-04-10
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