Date published: 2025-9-7
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surfactant protein c/SP-C Antibody (H-8) - Western Blotting - Image 397940
SP-C 抗体 (H-8): sc-518029. 非转染 (A) 和人 SP-C 转染 (B) 293T 全细胞裂解液中 SP-C 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
surfactant protein c/SP-C Antibody (H-8) - Western Blotting - Image 397940
SP-C 抗体 (H-8): sc-518029. 非转染 (A) 和人 SP-C 转染 (B) 293T 全细胞裂解液中 SP-C 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
surfactant protein c/SP-C Antibody (H-8) - Western Blotting - Image 386948
SP-C 抗体 (H-8): sc-518029. 非转染 (A) 和人 SP-C 转染 (B) HEK293T 全细胞裂解液中 SP-C 表达的 Western 印迹分析.
SP-C 抗体 (H-8): sc-518029. 非转染 (A) 和人 SP-C 转染 (B) 293T 全细胞裂解液中 SP-C 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

surfactant protein c/SP-C 抗体 (H-8): sc-518029

5.0(2)
寫評論提問

说明书
  • surfactant protein c/SP-C 抗体 H-8 是小鼠单克隆 IgG2b κ，surfactant protein c/SP-C抗体, 在35篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的的C-terminus附近的氨基酸121-146之间
  • surfactant protein c/SP-C 抗体 (H-8) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 SP-C precursor
  • 抗surfactant protein c/SP-C抗体（H-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取surfactant protein c/SP-C (H-8): sc-518029的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是surfactant protein c/SP-C Antibody (H-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与surfactant protein c/SP-C Antibody (H-8) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Pulmonary Surfactant-Associated Protein抗体，包括SP-A, SP-B, SP-C和SP-D

SP-C抗体（H-8）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人源SP-C蛋白。SP-C (H-8) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。SP-C蛋白在肺表面活性物的形成和功能中起着至关重要的作用，而肺表面活性物对于降低肺泡中气液界面的表面张力至关重要，从而防止呼气时肺泡塌陷，促进正常呼吸。SP-C蛋白主要位于肺泡II型细胞的层状体中，在那里SP-C蛋白与脂质相互作用，形成稳定的表面活性层。SP-C蛋白的正常功能对于维持肺顺应性和有效的气体交换至关重要，而SP-C蛋白缺乏会导致严重的呼吸系统疾病，如新生儿呼吸窘迫综合征。此外，SP-C蛋白会经历翻译后修饰，包括棕榈酰化，从而增强SP-C蛋白的疏水性，促进SP-C蛋白整合到表面活性剂膜中，进一步强调了SP-C蛋白在肺健康中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

surfactant protein c/SP-C 参考文献:

  1. 人类表面活性物质多肽 SP-B。二硫桥, C 端和气道形式的肽分析。  |  Johansson, J., et al. 1992. FEBS Lett. 301: 165-7. PMID: 1568474
  2. 表面活性物质相关蛋白质的结构和特性。  |  Hawgood, S. and Shiffer, K. 1991. Annu Rev Physiol. 53: 375-94. PMID: 2042965
  3. 小鼠肺表面活性蛋白 SP-C 基因的结构和表达。  |  Glasser, SW., et al. 1990. J Biol Chem. 265: 21986-91. PMID: 2254341
  4. 肺表面活性物质的分子和细胞加工。  |  Rooney, SA., et al. 1994. FASEB J. 8: 957-67. PMID: 8088461
  5. 人类表面活性蛋白 D（SP-D）的基因组结构。SP-D 在染色体 10q22.2-23.1 上编码。  |  Crouch, E., et al. 1993. J Biol Chem. 268: 2976-83. PMID: 8428971
  6. 肺表面活性物质成分的分子结构和相互作用。  |  Johansson, J. and Curstedt, T. 1997. Eur J Biochem. 244: 675-93. PMID: 9108235
  7. 肺表面活性蛋白 SP-A 和 SP-D 在先天性免疫中的功能作用。  |  Reid, KB. 1998. Immunobiology. 199: 200-7. PMID: 9777406

订购信息

产品名称产品编号规格价格数量收藏夹

surfactant protein c/SP-C 抗体 (H-8)

sc-518029
200 µg/ml
$316.00

surfactant protein c/SP-C (H-8): m-IgGκ BP-HRP 套装

sc-551120
200 µg Ab; 40 µg BP
$354.00

surfactant protein c/SP-C 抗体 (H-8) AC

sc-518029 AC
500 µg/ml, 25% agarose
$416.00

surfactant protein c/SP-C 抗体 (H-8) HRP

sc-518029 HRP
200 µg/ml
$316.00

surfactant protein c/SP-C 抗体 (H-8) FITC

sc-518029 FITC
200 µg/ml
$330.00

surfactant protein c/SP-C 抗体 (H-8) PE

sc-518029 PE
200 µg/ml
$343.00

surfactant protein c/SP-C 抗体 (H-8) Alexa Fluor® 488

sc-518029 AF488
200 µg/ml
$357.00

surfactant protein c/SP-C 抗体 (H-8) Alexa Fluor® 546

sc-518029 AF546
200 µg/ml
$357.00

surfactant protein c/SP-C 抗体 (H-8) Alexa Fluor® 594

sc-518029 AF594
200 µg/ml
$357.00

surfactant protein c/SP-C 抗体 (H-8) Alexa Fluor® 647

sc-518029 AF647
200 µg/ml
$357.00

surfactant protein c/SP-C 抗体 (H-8) Alexa Fluor® 680

sc-518029 AF680
200 µg/ml
$357.00

surfactant protein c/SP-C 抗体 (H-8) Alexa Fluor® 790

sc-518029 AF790
200 µg/ml
$357.00

I am reaching out to request a free 10 µg sample of your product, surfactant protein c/SP-C (H-8): sc-518029. If there are any specific details or procedures required to receive the sample, I would be grateful if you could let me know. Best regards

Asked by: Kurose
Thank you very much for your question. Please ask your Japanese dealer to apply for sample products. Website: www.cosmobio.co.jp ， www.iwai-chem.co.jp or www.nacalai.co.jp/ss/Contact/
Answered by: Jiajun Z
Date published: 2024-10-29

可以用于大鼠肺泡II型上皮细胞的鉴定吗？免疫荧光或者WB，同源性是多少呢？

Asked by: YJJJ
感谢您的提问。surfactant protein c/SP-C 抗体 (H-8) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 SP-C precursor。不推荐用于检测大鼠样本。谢谢。 Thank you for your question. surfactant protein c/SP-C Antibody (H-8) is recommended for use in WB, IP, IF and ELISA for detection of SP-C precursor of human origin. it is not recommended for detection of rat samples. Thank you.
Answered by: Jiajun Z
Date published: 2024-10-10

您好，这个抗体能做荧光吧，看上面都没有荧光图

Asked by: z123
感谢您的提问。surfactant protein c/SP-C Antibody (H-8): sc-518029 推荐用免疫荧光检测人源的细胞和冰冻组织切片的SP-C前体。 Thank you for your question. surfactant protein c/SP-C Antibody (H-8): sc-518029 is recommended for detection of SP-C precursor of human origin of cells and frozen tissue sections by IF.
Answered by: Technical Service SH
Date published: 2024-01-15

Has anyone used the conjugated version (SP-C (H-8) Alexa Fluor546 sc-518029) for IF? what would be the recommended dilution ratio?

Asked by: Claireee
Thank you for your question. The Alexa flour conjugated form can be used for IF and we recommend a starting dilution of 1:50.https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2023-11-30

Excuse me, can I use H-8 for immune capture of SP-C+ exosomes for constructing bead-bound exosomes? How can I eluting the antigen？ thanks.

Asked by: leeeli
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-11-23

excuse me, can I use H-8 for immune capture of SP-C+ exosme for bead-bound exosomes? Thanks.

Asked by: leeeli
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-11-23

Greetings, are there any publications where your sc-518029 antibody has been used in flow cytometry? If yes, what protocol is recommended?

Asked by: Stromgarde
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-09

可以用于小鼠肺泡II型上皮细胞的鉴定吗？免疫荧光或者WB

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-11-13
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Rated 5 out of 5 by from Specifically detects human SP-C by Western BlotAntibody shows a strong 20.8 KDa Western Blot band with a transfected lysate that over-expresses human SP-C isoform 2, variant 3. Transfected Lysate's NCBI Reference Sequence: NM_001172357.2 -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Strong band in HBEC cellsUsed this at a 1:500 concentration for western; a very clear strong band showed up at the expected size. Few non-specific bands were detected. Used in Human Bronchial Eptihelial cell lines.
Date published: 2018-04-18
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surfactant protein c/SP-C 抗体 (H-8) is rated 5.0 out of 5 by 2.
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