Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sox2 Double Nickase Plasmid (m2): sc-423086-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox2 Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sox2 Double Nickase Plasmid (m2) and Sox2 Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Sox2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox2 Antibody (E-4): sc-365823
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox2 Double Nickase Plasmid (m2) | sc-423086-NIC-2 | 20 µg | $410.00 |
Mouse Sox2 encodes the SRY-box transcription factor SOX2, a core regulator of pluripotency and self-renewal that cooperates with OCT4 and NANOG to maintain embryonic stem cell identity while restraining premature lineage commitment. SOX2 binds enhancer and promoter elements to control transcriptional programs governing neuroectoderm specification, neural stem/progenitor maintenance, and chromatin state transitions, interfacing with signaling inputs such as WNT, FGF, BMP, and SHH that shape developmental patterning. Dysregulated Sox2 expression or dosage is linked to defects in early embryogenesis and neurodevelopment, and altered SOX2 regulatory networks are frequently studied in the context of stem-like states in cancer biology. Gene editing of mouse Sox2 supports mechanistic interrogation of transcriptional circuitry, enhancer function, cell-fate decisions, and reprogramming workflows in developmental and disease-model systems.
Sox2 Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Sox2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sox2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sox2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sox2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.