Date published: 2025-9-6
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Sox2 Antibody (E-4) - Western Blotting - Image 394708
Sox-2 抗体 (E-4) Alexa Fluor® 488: sc-365823 AF488. F9 (A), H69AR (B) 和 C6 (C) 全细胞裂解液中 Sox-2 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
Sox-2 抗体 (E-4) Alexa Fluor® 488: sc-365823 AF488. F9 (A), H69AR (B) 和 C6 (C) 全细胞裂解液中 Sox-2 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

Sox2 抗体 (E-4): sc-365823

4.1(42)
寫評論提問

说明书
  • Sox2 抗体 E-4 是小鼠单克隆 IgG1 κ，Sox2抗体, 在359篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Sox-2的内部区域内的氨基酸170-201之间
  • Sox2 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Sox-2; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗Sox2抗体（E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-365823 X, 200 µg/0.1 ml)
  • 关于如何获取Sox2 (E-4): sc-365823的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Sox2 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Sox2 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Sox 抗体 包括 Sox-10, Sox-13, Sox-15, Sox-17, Sox-18, Sox-2, Sox-21, Sox-22, Sox-3和Sox-30

Sox-2抗体（E-4）是一种IgG1 κ小鼠单克隆Sox-2抗体（也被指定为SOX2抗体或SRY相关HMG盒转录因子2抗体），可通过WB、IP、IF、IHC（P）和ELISA方法检测小鼠、大鼠和人类来源的Sox-2蛋白。Sox-2抗体（E-4）既可以非偶联的抗Sox-2抗体形式提供，也可以多种偶联形式的抗Sox-2抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Sox基因是一个与哺乳动物性别决定基因SRY相关的基因家族。这些基因同样包含编码HMG盒结构域的序列，该结构域负责序列特异性的DNA结合活性。Sox基因编码推定的转录调控因子，这些因子在发育过程中决定细胞命运和控制多种发育过程中发挥作用。高度复杂的Sox基因组至少聚集了40个在不同动物谱系中迅速分化的不同位点。目前，已经鉴定出30个Sox基因。这个家族的成员在进化过程中被证明是保守的，并在动物发育过程中发挥关键作用。其中一些与人类疾病有关，包括性反转。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sox2 抗体 (E-4) 参考文献:

  1. 鉴定新型 Sry 相关基因及其生殖细胞特异性表达。  |  Osaki, E., et al. 1999. Nucleic Acids Res. 27: 2503-10. PMID: 10359848
  2. Sox 因子在神经决定过程中的作用：进化中的保守信号传递？  |  Sasai, Y. 2001. Int J Dev Biol. 45: 321-6. PMID: 11291862
  3. HMG 盒超家族的起源和多样性。  |  Laudet, V., et al. 1993. Nucleic Acids Res. 21: 2493-501. PMID: 8506143
  4. 神经胶质细胞中的新型转录调节器 Sox10  |  Kuhlbrodt, K., et al. 1998. J Neurosci. 18: 237-50. PMID: 9412504
  5. 人类 SOX14 基因的特征和图谱。  |  Arsic, N., et al. 1998. Cytogenet Cell Genet. 83: 139-46. PMID: 9925951

订购信息

产品名称产品编号规格价格数量收藏夹

Sox2 抗体 (E-4)

sc-365823
200 µg/ml
$316.00

Sox2 (E-4): m-IgG Fc BP-HRP 套装

sc-529482
200 µg Ab; 10 µg BP
$354.00

Sox2 (E-4): m-IgGκ BP-HRP 套装

sc-522383
200 µg Ab, 40 µg BP
$354.00

Sox2 (E-4): m-IgG1 BP-HRP 套装

sc-543536
200 µg Ab; 20 µg BP
$354.00

Sox2 抗体 (E-4) X

sc-365823 X
200 µg/0.1 ml
$316.00

Sox2 抗体 (E-4) AC

sc-365823 AC
500 µg/ml, 25% agarose
$416.00

Sox2 抗体 (E-4) HRP

sc-365823 HRP
200 µg/ml
$316.00

Sox2 抗体 (E-4) FITC

sc-365823 FITC
200 µg/ml
$330.00

Sox2 抗体 (E-4) PE

sc-365823 PE
200 µg/ml
$343.00

Sox2 抗体 (E-4) Alexa Fluor® 488

sc-365823 AF488
200 µg/ml
$357.00

Sox2 抗体 (E-4) Alexa Fluor® 546

sc-365823 AF546
200 µg/ml
$357.00

Sox2 抗体 (E-4) Alexa Fluor® 594

sc-365823 AF594
200 µg/ml
$357.00

Sox2 抗体 (E-4) Alexa Fluor® 647

sc-365823 AF647
200 µg/ml
$357.00

Sox2 抗体 (E-4) Alexa Fluor® 680

sc-365823 AF680
200 µg/ml
$357.00

Sox2 抗体 (E-4) Alexa Fluor® 790

sc-365823 AF790
200 µg/ml
$357.00

Sox2 (E-4) 中和勝肽

sc-365823 P
100 µg/0.5 ml
$68.00

Sox2 is expressed in full length and a C-terminal fragment (~amino acids 180-317; https://www.sciencedirect.com/science/article/pii/S2211124724011586). Would the E-4 Sox2 antibody be expected to bind to full-length and the C-terminal fragment?

Asked by: Keith
Thank you for your question. Sox-2 (E-4) has an epitope range of 170-201. Sox-2 (E-4) would not bind to the C-terminal fragment.
Answered by: BlakeJ
Date published: 2024-12-12

What is the host species of this antibody?

Asked by: Mercy7
Thank you for your question. This Sox-2 antibody is a mouse monoclonal antibody raised against human Sox-2. It is mouse, rat and human reactive.
Answered by: Tech Service
Date published: 2019-03-13

Can I use Sox-2 Antibody (E-4): sc-365823 mouse monoclonal antibody for immunohistochemical staining of CHIP assay?

Asked by: hushi01
Thank you for your question. We recommend this mouse monoclonal antibody, Sox-2 (E-4): sc-365823, for IHC(P) or immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. We are not yet aware of any publications citing use of this antibody for ChIP or Chromatin Immunoprecipitation assays.
Answered by: Technical Support
Date published: 2020-11-26

Can I use Sox-2 Antibody (E-4): sc-365823 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: AbPolly
Yes. We recommend using a directly conjugated monoclonal antibody. NCAM (123C3): sc-7326 monoclonal antibody is available directly conjugated to either HRP (sc-365823 HRP), phycoerythrin (sc-365823 PE), fluorescein (sc-365823 FITC), Alexa Fluor® 488 (sc-365823 AF488), Alexa Fluor® 647 (sc-365823 AF647).
Answered by: Technical Support 5
Date published: 2017-01-10

When should the TransCruz reagent sc-365823 X be used instead of the standard form sc-365823?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. Sox-2 Antibody (E-4): sc-365823 X form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 12
Date published: 2017-01-10

I am working with porcine samples. Can this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2017-01-04
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Santa抗体试用装太棒了，我想申请要个试用装我感觉Santa抗体的量好大，正品是1ml，试用装都有50ul，效价和稀释比也很好，真是很值得。抗体特异性是不错的，这个还是看选的抗体，我之前购买的几个还是都很不错的。
Date published: 2024-04-01
Rated 5 out of 5 by from Good antibody for western blotThis antibody detects Sox2 at the expected molecular weight
Date published: 2022-06-17
Rated 5 out of 5 by from Very good antibody for SOX2We have used this Ab to observe endogenous SOX2 on ES extracts and after overexpression on primary cells by Western blot with very good results
Date published: 2021-11-19
Rated 4 out of 5 by from buono funzionamentoHo provato recentemente l'anti-SOX2 e sono soddisfatto della specificità del reagente
Date published: 2021-11-06
Rated 5 out of 5 by from Very goodNicely stained hiPS cell 1:50 dilution 4℃, O/N staining
Date published: 2021-08-05
Rated 1 out of 5 by from Not working in IHC.We recently bought this antibody for IHC application. We use at 1:50 dilution but didn't get any staining. We are still trying to get staining using different dilution but not anything. Thank you !!
Date published: 2020-09-16
Rated 5 out of 5 by from GreatI used this antibody for IF, it works well for hMSCs
Date published: 2019-07-27
Rated 5 out of 5 by from Very good for both IF and WBVery good for both immunofluorescence and western blotting
Date published: 2019-04-09
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Sox2 抗体 (E-4) is rated 4.1 out of 5 by 42.
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