Date published: 2025-9-7
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SOCS-3 Antibody (6A463) - Western Blotting - Image 73952
SOCS-3 抗体 (6A463): sc-73045. 人 PBL (A) 和 A549 (B) 全细胞裂解液中 SOCS-3 表达的 Western 印迹分析.
SOCS-3 Antibody (6A463) - Western Blotting - Image 73952
SOCS-3 抗体 (6A463): sc-73045. 人 PBL (A) 和 A549 (B) 全细胞裂解液中 SOCS-3 表达的 Western 印迹分析.
SOCS-3 Antibody (6A463) - Western Blotting - Image 368018
SOCS-3 抗体 (6A463): sc-73045. HeLa (A), Sol8 (B) 和 KNRK (C) 全细胞裂解液中 SOCS-3 表达的 Western 印迹分析.
SOCS-3 Antibody (6A463) - Western Blotting - Image 81138
SOCS-3 抗体 (6A463): sc-73045. 对 HeLa 全细胞裂解液中 SOCS-3 的表达进行 Western 印迹分析.
SOCS-3 抗体 (6A463): sc-73045. 人 PBL (A) 和 A549 (B) 全细胞裂解液中 SOCS-3 表达的 Western 印迹分析.

SOCS-3 抗体 (6A463): sc-73045

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说明书
  • SOCS-3 抗体 6A463 是小鼠单克隆 IgG2b κ，SOCS-3抗体, 在20篇文献中引用，规格为100 µg/ml
  • 针对纯化的human全长天然SOC3蛋白
  • 推荐用于 mouse, rat 和 human 来源的SOCS-3 WB, IP 和 ELISA检测
  • 关于如何获取SOCS-3 (6A463): sc-73045的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成SOCS-3 抗体 (6A463)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

此外 SOCS 抗体包括 SOCS-1, SOCS-3, SOCS-4, SOCS-5, SOCS-6 和 SOCS-7

SOCS-3抗体（6A463）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的SOCS-3蛋白。抗SOCS-3抗体（6A463）以非结合形式提供。SOCS-3蛋白在细胞因子信号的负调控中起着至关重要的作用，而细胞因子信号对于维持免疫平衡和防止过度炎症反应至关重要。SOCS-3通过抑制JAK/STAT信号传导通路发挥作用，尤其是对白细胞介素-6（IL-6）和其他细胞因子的反应。这种调节至关重要，因为SOCS-3失调会导致各种病理状况，包括自身免疫性疾病和癌症。SOCS-3的结构特征是中央SH2结构域和独特的羧基末端SOCS盒，它们对于与JAK和其他信号分子的相互作用至关重要，从而调节信号级联。SOCS-3 (6A463)抗体使研究人员能够深入了解细胞因子信号传导的机制及其对健康和疾病的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SOCS-3 抗体 (6A463) 参考文献:

  1. 血管紧张素 II（AngII）诱导大鼠下丘脑中细胞因子信号转导抑制因子（SOCS）-3的表达--AngII信号转导的脱敏机制。  |  Torsoni, MA., et al. 2004. J Endocrinol. 181: 117-28. PMID: 15072572
  2. 细胞因子信号转导抑制蛋白（SOCS）的不同活性以及 SOCS 盒参与控制 G-CSF 信号转导。  |  van de Geijn, GJ., et al. 2004. J Leukoc Biol. 76: 237-44. PMID: 15107455
  3. SOCS-1和SOCS-3抑制IL-4和IL-13诱导的Eotaxin-3/CCL26基因表达在HEK293细胞中的激活。  |  Hebenstreit, D., et al. 2005. Mol Immunol. 42: 295-303. PMID: 15589317
  4. 抵抗素激活SOCS-3。  |  Steppan, CM., et al. 2005. Mol Cell Biol. 25: 1569-75. PMID: 15684405
  5. 无偏倚鉴定Epac1诱导的E3泛素连接酶成分SOCS-3的底物。  |  Williams, JJ. and Palmer, TM. 2012. Biochem Soc Trans. 40: 215-8. PMID: 22260693
  6. 在肾积水大鼠模型中，通过减轻肾小管损伤，沉默SOCS-1和SOCS-3抑制了肾间质纤维化。  |  Zheng, GH., et al. 2018. J Cell Biochem. 119: 2200-2211. PMID: 28857282
  7. SOCS-3有助于双酚a暴露诱导的肝细胞胰岛素抵抗。  |  Qi, HM., et al. 2019. Sci China Life Sci. 62: 1597-1600. PMID: 31802416
  8. 一种新型细胞因子诱导基因 CIS 编码一种含 SH2 的蛋白质，它能与酪氨酸磷酸化的白细胞介素 3 和促红细胞生成素受体结合。  |  Yoshimura, A., et al. 1995. EMBO J. 14: 2816-26. PMID: 7796808
  9. CIS是一种细胞因子诱导型SH2蛋白，是JAK-STAT5通路的靶标，可调节STAT5的激活。  |  Matsumoto, A., et al. 1997. Blood. 89: 3148-54. PMID: 9129017
  10. 细胞因子诱导信号抑制剂家族。  |  Starr, R., et al. 1997. Nature. 387: 917-21. PMID: 9202125
  11. 20 个含有 C 端 SOCS 框的蛋白质形成了 5 个结构类别。  |  Hilton, DJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 114-9. PMID: 9419338
  12. SOCS 蛋白：信号转导负调控因子的新家族。  |  Nicholson, SE. and Hilton, DJ. 1998. J Leukoc Biol. 63: 665-8. PMID: 9620657

订购信息

产品名称产品编号规格价格数量收藏夹

SOCS-3 抗体 (6A463)

sc-73045
100 µg/ml
$316.00

I could not detect endogenous SOCS3 expression in mesothelioma cell-lines with this antibody. What are the optimal conditions for protein detection in human cancer cell lines?

Asked by: Aish K
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-09-13

Whats the species of origin? I mean, in which species the antibody was raised.

Asked by: Faraz A
Thank you for your question. SOCS-3 Antibody (6A463) is a mouse monoclonal IgG2b.
Answered by: Technical Support
Date published: 2019-02-23

What is the recommended storage condition for SOCS-3 (6A463): sc-73045?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27

Used the antibody at 1:500 dilution on Western blot of RAW264 cell lysate. Saw two bands very close together between 38 to 28 kDa, with the upper band a bit more intense than the lower band. Does this antibody detect only SOCS3 or also other SOCS?

Asked by: JanetL
Thank you for your question. This mouse monoclonal, SOCS-3 Antibody (6A463): sc-73045, is only recommended for detection of SOCS-3. We have not specifically tested the antibody for cross-reactivity with other SOCS family members. The expected molecular weight of SOCS-3 is approximately 30 kDa. We have not tested this antibody in RAW 264.7 cell lysate, but in other cell lines tested we did not observe the same signals you have described. We would recommend performing a secondary antibody control (omit primary antibody) to identify if the additional band is a result of non-specific binding from the secondary antibody alone.
Answered by: Technical Support
Date published: 2018-05-15
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Rated 5 out of 5 by from 非常好用的抗体前段时间我的一个师兄买了贵公司的一个stat3的抗体，孵育出来的条带特异性强，没有任何杂带，所以想再申请一个socs3的试用装，看下效果。
Date published: 2018-09-12
Rated 5 out of 5 by from good WB resultsstrong and specific band in western blot with mice liver lysates, dilution 1:1000
Date published: 2018-09-05
Rated 5 out of 5 by from Very strongIt can work in human cell lines, but can not in mouse MEFs. The band is very strong in DLD1 cell line.
Date published: 2018-02-06
Rated 4 out of 5 by from worked well in western blottested on rat tissue samples and gave a nice band in western blot.
Date published: 2017-12-13
Rated 5 out of 5 by from good resultThis antibody is very specific,The result is very good!
Date published: 2017-10-23
Rated 5 out of 5 by from sc-73045I have used sc-73045 sample for western blot. The figures were nice.
Date published: 2017-10-23
Rated 5 out of 5 by from A good antibody to detect liver tissue of mouseA 10 ug sample, got a single and clear band around 25 kDa.
Date published: 2017-09-27
Rated 3 out of 5 by from not so good as expectedApplied for guinea pig liver samples. Two bands were detected among 25-35KD. For the first time, signal was strong. But for next few times, bands were not clear, more not specific bands beyond 35KD were seen and there was heavy background.
Date published: 2017-09-07
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SOCS-3 抗体 (6A463) is rated 4.7 out of 5 by 16.
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